Rat alpha 1-Microglobulin ELISA Kit (Colorimetric)

ELISA: Rat alpha 1-Microglobulin ELISA Kit (Colorimetric) [NBP3-00486] - Samples were spiked with high concentrations of Rat alpha 1-Microglobulin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat alpha 1-Microglobulin ELISA Kit (Colorimetric) [NBP3-00486] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 6.25 - 400 ng/mL
• Sensitivity: 3.75 ng/mL

Rat alpha 1-Microglobulin ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat alpha 1-Microglobulin in samples. No significant cross-reactivity or interference between Rat alpha 1-Microglobulin and analogues was observed.
• Standard Curve Range: 6.25 - 400 ng/mL
• Sensitivity: 3.75 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: 8.6%
• Intra-Assay: 10.34%
• Spike Recovery: 85 - 105%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: AMBP

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat alpha 1-Microglobulin ELISA Kit (Colorimetric)

• A1M
• alpha 1Microglobulin
• alpha 1-Microglobulin
• alpha-1-microglobulin/bikunin precursor
• AMBP
• bikunin
• EDC1
• HCP
• HCPcomplex-forming glycoprotein heterogeneous in charge
• HI30
• IATIL
• inter-alpha-trypsin inhibitor light chain
• ITI
• ITIL
• ITILC
• ITILgrowth-inhibiting protein 19
• protein AMBP
• protein HC
• trypstatin
• uristatin
• uronic-acid-rich protein
• UTI

Background

Alpha-1-Microglobulin encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for alpha 1-Microglobulin ELISA Kit (NBP3-00486). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: AMBP