alpha 1-Microglobulin Antibody

Western Blot: alpha 1-Microglobulin Antibody [NBP2-76910] - Western blot analysis of AMBP on different cell lysates using anti-AMBP antibody at 1/1,000 dilution.Positive control:Lane 1: HepG2Lane 2: Human liverLane 3: Mouse liverLane 4: Mouse brain

Immunocytochemistry/Immunofluorescence: alpha 1-Microglobulin Antibody [NBP2-76910] - ICC staining AMBP in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: alpha 1-Microglobulin Antibody [NBP2-76910] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-AMBP antibody. Counter stained with hematoxylin.

Flow Cytometry: alpha 1-Microglobulin Antibody [NBP2-76910] - Flow cytometric analysis of HepG2 cells with AMBP antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: alpha 1-Microglobulin Antibody [NBP2-76910] - ICC staining AMBP in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: alpha 1-Microglobulin Antibody [NBP2-76910] - ICC staining AMBP in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: alpha 1-Microglobulin Antibody [NBP2-76910] - Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-AMBP antibody. Counter stained with hematoxylin.

Immunohistochemistry: alpha 1-Microglobulin Antibody [NBP2-76910] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-AMBP antibody. Counter stained with hematoxylin.

Product Discontinued

alpha 1-Microglobulin Antibody Summary

• Immunogen: Recombinant protein within Human alpha 1-Microglobulin aa 120-320. (SwissProt: P02760 Human)
• Localization: Secreted.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AMBP
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for alpha 1-Microglobulin Antibody

• A1M
• alpha 1Microglobulin
• alpha 1-Microglobulin
• alpha-1-microglobulin/bikunin precursor
• AMBP
• bikunin
• EDC1
• HCP
• HCPcomplex-forming glycoprotein heterogeneous in charge
• HI30
• IATIL
• inter-alpha-trypsin inhibitor light chain
• ITI
• ITIL
• ITILC
• ITILgrowth-inhibiting protein 19
• protein AMBP
• protein HC
• trypstatin
• uristatin
• uronic-acid-rich protein
• UTI

Background

Alpha-1-Microglobulin encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AMBP