alpha 1-Microglobulin Antibody (784917) [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 784917
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 405

alpha 1-Microglobulin Antibody (784917) [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human alpha 1‑Microglobulin
  Gly20-Val203
  Accession # P02760
• Specificity: Detects human alpha 1‑Microglobulin in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant rat alpha 1-Microglobulin is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha 1-Microglobulin Antibody (784917) [Alexa Fluor® 405]

• A1M
• alpha 1Microglobulin
• alpha 1-Microglobulin
• alpha-1-microglobulin/bikunin precursor
• AMBP
• bikunin
• EDC1
• HCP
• HCPcomplex-forming glycoprotein heterogeneous in charge
• HI30
• IATIL
• inter-alpha-trypsin inhibitor light chain
• ITI
• ITIL
• ITILC
• ITILgrowth-inhibiting protein 19
• protein AMBP
• protein HC
• trypstatin
• uristatin
• uronic-acid-rich protein
• UTI

Background

Human alpha 1-Microglobulin (alpha1-m/A1M; also protein HC) is a secreted, 31-32 kDa glycoprotein member of the lipocalin family, calycin superfamily of molecules. It is expressed by hepatocytes, keratinocytes, and endodermal derivatives in the embryo.  alpha 1-Microglobulin appears to act as a heme scavenger, protecting cells and collagen against oxidative damage. It also acts as an immunosuppressant, inhibiting polyclonal lymphocyte activation and dampening granulocyte migration in response to chemokines. alpha 1-Microglobulin circulates either as a monomer, or bound to IgA, albumin or prothrombin. Human alpha 1-Microglobulin is generated through cleavage of a precursor molecule termed AMBP. This AMBP should not be confused with AMBP-1, a 120-140 kDa adrenomedullin-binding protein that is also known as Complement Factor H. The AMBP precursor contains a 19 aa signal sequence, an N-terminal 183 aa alpha 1-Microglobulin protein (aa 20-203), and a C-terminal serine protease inhibitor termed bikunin (aa 206-352). alpha 1-Microglobulin possesses one lipocalin domain (aa 42-186). Although cleavage of AMBP in the Golgi apparatus typically generates a 31 kDa alpha 1-Microglobulin and 28 kDa bikunin molecule, the 60-65 kDa AMBP precursor can also be released intact.  alpha 1-Microglobulin will undergo extracellular processing, generating a 30 kDa isoform that is missing aa 199-203. There is one splice variant that shows a deletion of aa 48-57.  Over aa 20-203, human alpha 1-Microglobulin shares 76% aa sequence identity with both mouse and rat alpha 1-Microglobulin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P02760(Human)