Recombinant Human ALKBH3 Protein

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SDS-Page: Recombinant Human ALKBH3 Protein [NBP1-51051] - 15 % SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human ALKBH3 Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-286 of Human ALKBH3

Source: E.coli

Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELDM EEKRRRARVQ GAWAAPVKSQ AIAQPATTAK SHLHQKPGQT WKNKEHHLSD REFVFKEPQQ VVRRAPEPRV IDREGVYEIS LSPTGVSRVC LYPGFVDVKE ADWILEQLCQ DVPWKQRTGI REDITYQQPR LTAWYGELPY TYSRITMEPN PHWHPVLRTL KNRIEENTGH TFNSLLCNLY RNEKDSVDWH SDDEPSLGRC PIIASLSFGA TRTFEMRKKP PPEENGDYTY VERVKIPLDH GTLLIMEGAT QADWQHRVPK EYHSREPRVN LTFRTVYPDP RGAPW

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
ALKBH3
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Theoretical MW
37.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Human ALKBH3 Protein

  • ABH3MGC118793
  • alkB, alkylation repair homolog 3 (E. coli)
  • Alkylated DNA repair protein alkB homolog 3
  • DEPC1alpha-ketoglutarate-dependent dioxygenase alkB homolog 3
  • DEPC-1MGC118792
  • EC 1.14.11
  • EC 1.14.11.-
  • FLJ43614
  • MGC118790
  • PCA1
  • Prostate cancer antigen 1
  • prostate cancer antigen-1

Background

ALKBH3 is a member of the ALKB family of proteins and functions as a dioxygenase with a preference for RNA and single stranded DNA substrates. This protein repairs 1-methyladenine and 3-methylcytosine lesions in alkylated DNA and RNA and its activity is stimulated by ascorbate. ALKBH3 is expressed in a wide variety of tissues and localizes to the cytoplasm and the nucleus. Recombinant human ALKBH3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol ALKBH3