ALKBH3 Antibody

Immunocytochemistry/Immunofluorescence: ALKBH3 Antibody [NBP1-84274] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & mitochondria.

Immunohistochemistry-Paraffin: ALKBH3 Antibody [NBP1-84274] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: ALKBH3 Antibody [NBP1-84274] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.

Immunohistochemistry-Paraffin: ALKBH3 Antibody [NBP1-84274] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.

Immunohistochemistry-Paraffin: ALKBH3 Antibody [NBP1-84274] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.

• Reactivity: Hu
• Applications: ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

ALKBH3 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: WAAPVKSQAIAQPATTAKSHLHQKPGQTWKNKEHHLSDREFVFKEPQQVVRRAPEPRVIDREGVYEISLSPTGVSRVCLYPGFVDVKEADWILEQLCQDVPWKQRTGIREDITYQQPRLTAWYGEL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ALKBH3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  ALKBH3 Protein (NBP1-84274PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ALKBH3 Antibody

• ABH3MGC118793
• alkB, alkylation repair homolog 3 (E. coli)
• Alkylated DNA repair protein alkB homolog 3
• DEPC1alpha-ketoglutarate-dependent dioxygenase alkB homolog 3
• DEPC-1MGC118792
• EC 1.14.11
• EC 1.14.11.-
• FLJ43614
• MGC118790
• PCA1
• Prostate cancer antigen 1
• prostate cancer antigen-1

Background

The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 (MIM 610602) and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALKBH3
• Entrez:
  • 221120(Human)
• Uniprot:
  • Q96Q83(Human)