Alkaline Phosphatase, Tissue Non-Specific Native Protein Summary
Preparation Method |
Native protein purified from E. coli |
| Details of Functionality |
Greater than or equal to 10 units per mg protein (25C, pH 8.0). One Unit hydrolyzes 1 umole of p-nitrophenol phosphate per minute at 25C, pH 8.0. |
| Protein/Peptide Type |
Native Protein |
| Gene |
ALPL |
Applications/Dilutions
| Application Notes |
Solution. Partially purified. |
Reactivity Notes
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles. |
| Buffer |
2.6 M ammonium sulfate, pH 8.0 |
| Preservative |
No Preservative |
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Alkaline Phosphatase, Tissue Non-Specific Native Protein
Background
Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 1 year from date of receipt.
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