Alkaline Phosphatase, Tissue Non-Specific Antibody (EPR4477)

Product Discontinued

Alkaline Phosphatase, Tissue Non-Specific Antibody (EPR4477) Summary

• Immunogen: A synthetic peptide corresponding to residues in human ALPL was used as an immunogen.
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ALPL
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:250-1:500
  • Immunoprecipitation 1:10-1:100
  • Simple Western
  • Western Blot 1:10000-1:50000
• Application Notes: In Western blot, detects a band at ~80 kDa.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for Alkaline Phosphatase, Tissue Non-Specific Antibody (EPR4477)

• Alkaline phosphatase liver/bone/kidney isozyme
• alkaline phosphatase, liver/bone/kidney
• alkaline phosphatase, tissue-nonspecific isozyme
• alkaline phosphomonoesterase
• APTNAP
• AP-TNAP
• EC 3.1.3.1
• FLJ40094
• FLJ93059
• glycerophosphatase
• HOPS
• liver/bone/kidney-type alkaline phosphatase
• MGC161443
• tissue-nonspecific ALP
• TNAP
• TNSALPMGC167935

Background

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. ALPL is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Alkaline Phosphatase, Tissue Non-Specific Antibody (NBP1-95392)(2)

Publications using NBP1-95392

• Verrier JD, Kochanek PM, Jackson EK. Schwann Cells Metabolize Extracellular 2',3'-cAMP to 2'-AMP. J. Pharmacol. Exp. Ther. 2015-05-21 [PMID: 25998049] (WB, ICC/IF, Rat)
• Jackson EK, Cheng D, Verrier JD et al. Interactive Roles of CD73 and Tissue Non-Specific Alkaline Phosphatase in the Renal Vascular Metabolism of 5'-AMP. Am. J. Physiol. Renal Physiol. 2014-07-02 [PMID: 24990899] (WB, Mouse)
  
  Details:
  Alkaline Phosphatase, Tissue Non-Specific / TNAP antibody used for WB on lysates of kidneys from CD73-/- mice on C57BL/6 x J129 background and wild-type litter mates CD73+/+ mice (Fig 4B).

Reviews for Alkaline Phosphatase, Tissue Non-Specific Antibody (NBP1-95392) (3)

Average Rating: 4.7

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Human.

reviewed by: Verified Customer ICC Human 06/11/2014

Summary

• Application: Immunocytochemistry
• Sample Tested: Human renal sections
• Species: Human

reviewed by: Verified Customer IF Human 06/11/2014

Summary

• Application: Immunofluorescence
• Sample Tested: Human renal sections
• Species: Human

reviewed by: Verified Customer ICC Human 04/29/2014

Summary

• Application: Immunocytochemistry
• Sample Tested: Human renal sections
• Species: Human

Bioinformatics

• Gene Symbol: ALPL
• Entrez:
  • 249(Human)
  • 11647(Mouse)
  • 25586(Rat)
• Uniprot:
  • P05186()