![SDS-Page: Aldo-keto Reductase 1C1/AKR1C1 Recombinant Protein [NBP2-52275] - 15% SDS Page (3 ug)](http://images.novusbio.com/fullsize2/Aldo-keto-Reductase-1C1-AKR1C1-Recombinant-Protein-SDS-Page-NBP2-52275-img0001.jpg "SDS-Page: Aldo-keto Reductase 1C1/AKR1C1 Recombinant Protein [NBP2-52275] - 15% SDS Page (3 ug)")
| Reactivity | HuSpecies Glossary |
| Applications | Func, PAGE, Bioactivity |
| Concentration | 1 mg/ml |
| Description | An un-tagged recombinant protein corresponding to the amino acids 1-323 of Human Aldo-keto Reductase 1C1/AKR1C1 Source: E.coli Amino Acid Sequence: MDSKYQCVKL NDGHFMPVLG FGTYAPAEVP KSKALEATKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCNSH RPELVRPALE RSLKNLQLDY VDLYLIHFPV SVKPGEEVIP KDENGKILFD TVDLCATWEA VEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNQR KLLDFCKSKD IVLVAYSALG SHREEPWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTS EEMKAIDGLN RNVRYLTLDI FAGPPNYPFS DEY |
| Specificity | Specific activity is > 500 pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole 1-Acenaphthenol the presence of NADP per minute at pH 8.8 at 25C. |
| Details of Functionality | Specific activity is > 500 pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25C. |
| Source | E. coli |
| Protein/Peptide Type | Recombinant Protein |
| Gene | AKR1C1 |
| Purity | >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain |
| Endotoxin Note | < 1.0 EU per 1ug of protein (determined by LAL method) |
| Theoretical MW | 36.7 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 20mM Tris-HCl buffer (pH 8.5) containing 0.1M NaCl, 20% glycerol |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain |
Diseases for Aldo-keto Reductase 1C1/AKR1C1 Recombinant Protein (NBP2-52275)Discover more about diseases related to Aldo-keto Reductase 1C1/AKR1C1 Recombinant Protein (NBP2-52275).
| Pathways for Aldo-keto Reductase 1C1/AKR1C1 Recombinant Protein (NBP2-52275)View related products by pathway.
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PTMs for Aldo-keto Reductase 1C1/AKR1C1 Recombinant Protein (NBP2-52275)Learn more about PTMs related to Aldo-keto Reductase 1C1/AKR1C1 Recombinant Protein (NBP2-52275).
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![Flow (Intracellular): Complement C3 Antibody (11H9) [NB200-540] - An intracellular stain was performed on RAW 246.7 cells with Complement C3 (11H9-3-2) antibody NB200-540 (blue) and a matched isotype control NBP2-31382 (orange). Cells were either treated with 3uM Monensin for 3 hours to block the secretion of Complement C3 (B) or grown in normal media (A). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).](http://images.novusbio.com/fullsize2/Complement-C3-Antibody-(11H9)-Flow-(Intracellular)-NB200-540-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (11H9) [NB200-540] - C3 protein fragments deposited on kidney cells of MPL-lpr mouse. Staining with antibody 11H9. Glomerular staining pattern.](http://images.novusbio.com/fullsize2/Complement-C3-Antibody-(11H9)-Immunocytochemistry-Immunofluorescence-NB200-540-img0002.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody (11H9) [NB200-540] - IHC-P detection of Complement C3 protein in a formalin fixed paraffin embedded tissue section of mouse liver using 1 : 100 dilution of Complement C3 antibody (clone 11H9) NB200-540. Weak but distinct membrane-cytoplasmic immunopositivity was observed in hepatocytes and few cells developed punctate membrane staining.](http://images.novusbio.com/fullsize2/Complement-C3-Antibody-(11H9)-Immunohistochemistry-Paraffin-NB200-540-img0004.jpg)
![Immunohistochemistry-Paraffin: Complement C7 Antibody [NBP1-88119] - Staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize2/Complement-C7-Antibody-Immunohistochemistry-Paraffin-NBP1-88119-img0001.jpg)
![Western Blot: Complement C6 Antibody [NBP2-30078] - Western blot analysis of C6 Antibody (N-term) (NBP2-30078) in MDA-MB231 cell line lysates (35ug/lane). C6 (arrow) was detected using the purified Pab.](http://images.novusbio.com/fullsize2/Complement-C6-Antibody-Western-Blot-NBP2-30078-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Complement C6 Antibody [NBP2-30078] - Confocal immunofluorescent analysis of C6 Antibody (N-term)(NBP2-30078) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).](http://images.novusbio.com/fullsize2/Complement-C6-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-30078-img0004.jpg)
![Immunohistochemistry-Paraffin: Complement C6 Antibody [NBP2-30078] - Formalin-fixed and paraffin-embedded human breast carcinoma reacted with C6 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.](http://images.novusbio.com/fullsize2/Complement-C6-Antibody-Immunohistochemistry-Paraffin-NBP2-30078-img0002.jpg)
![Western Blot: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - PSMA7 monoclonal antibody (M01), clone 1A10-3G12. Analysis of PSMA7 expression in human omentum, serous carcinoma.](http://images.novusbio.com/fullsize2/PSMA7-Antibody-(1A10-3G12)-Western-Blot-H00005688-M01-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Analysis of monoclonal antibody to PSMA7 on HeLa cell. Antibody concentration 1 ~ 10 ug/ml.](http://images.novusbio.com/fullsize2/PSMA7-Antibody-(1A10-3G12)-Immunocytochemistry-Immunofluorescence-H00005688-M01-img0009.jpg)
![Immunohistochemistry-Paraffin: PSMA7 Antibody (1A10-3G12) [H00005688-M01] - Analysis of monoclonal antibody to PSMA7 on formalin-fixed paraffin-embedded human colon tissue. Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize2/PSMA7-Antibody-(1A10-3G12)-Immunohistochemistry-Paraffin-H00005688-M01-img0013.jpg)
![Western Blot: hnRNP C1 + C2 Antibody (CL2593) [NBP2-36776] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HNRNPC antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize2/hnRNP-C1-+-C2-Antibody-(CL2593)-Western-Blot-NBP2-36776-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (CL2593) [NBP2-36776] - Staining in U2OS cell line with Anti-HNRNPC monoclonal antibody, showing distinct nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/hnRNP-C1-+-C2-Antibody-(CL2593)-Immunocytochemistry-Immunofluorescence-NBP2-36776-img0014.jpg)
![Immunohistochemistry: hnRNP C1 + C2 Antibody (CL2593) [NBP2-36776] - Staining of human testis shows nuclear positivity in seminiferous tubules.](http://images.novusbio.com/fullsize2/hnRNP-C1-+-C2-Antibody-(CL2593)-Immunohistochemistry-NBP2-36776-img0006.jpg)
![Western Blot: Complement C4a Antibody [NBP2-14893] - Sample (30 ug of whole cell lysate) A: HepG2; 5% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize2/Complement C4a Antibody-Western Blot-NBP2-14893-img0003.jpg)
![Immunohistochemistry-Paraffin: Complement C4a Antibody [NBP2-14893] - Paraffin-embedded Hepatoma, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize2/Complement-C4a-Antibody-Immunohistochemistry-Paraffin-NBP2-14893-img0001.jpg)
![Western Blot: AKR1C2 Antibody (3C11) [H00001646-M03] - Analysis of AKR1C2 expression in transfected 293T cell line by AKR1C2 monoclonal antibody (M03), clone 3C11. Lane 1: AKR1C2 transfected lysatE (36.7 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize2/AKR1C2 Antibody (3C11)-Western Blot-H00001646-M03-img0002.jpg)
![ELISA: AKR1C2 Antibody (3C11) [H00001646-M03] - Detection limit for recombinant GST tagged AKR1C2 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize2/AKR1C2-Antibody-(3C11)-ELISA-H00001646-M03-img0001.jpg)
![Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) Pacific BlueTM.](http://images.novusbio.com/fullsize2/CD59-Antibody-(MEM-43)-Flow-Cytometry-NB500-330-img0007.jpg)
![Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Analysis using the PE conjugate of NB500-330. Surface staining of human peripheral blood cells with anti-human CD59 (MEM-43) PE.](http://images.novusbio.com/fullsize2/CD59-Antibody-(MEM-43)-Flow-Cytometry-NB500-330-img0002.jpg)
![Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Using the PE direct conjugate Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) PE.](http://images.novusbio.com/fullsize2/CD59 Antibody (MEM-43)-Flow Cytometry-NB500-330-img0004.jpg)
![Western Blot: Aldo-keto Reductase 1C4/AKR1C4 Antibody (2C11) [H00001109-M01] - AKR1C4 monoclonal antibody (M01), clone 2C11 Analysis of AKR1C4 expression in MCF-7.](http://images.novusbio.com/fullsize2/Aldo-keto-Reductase-1C4-AKR1C4-Antibody-(2C11)-Western-Blot-H00001109-M01-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Aldo-keto Reductase 1C4/AKR1C4 Antibody (2C11) [H00001109-M01] - Analysis of monoclonal antibody to AKR1C4 on HepG2 cell. Antibody concentration 15 ug/ml.](http://images.novusbio.com/fullsize2/Aldo-keto-Reductase-1C4-AKR1C4-Antibody-(2C11)-Immunocytochemistry-Immunofluorescence-H00001109-M01-img0011.jpg)
![Immunohistochemistry-Paraffin: Aldo-keto Reductase 1C4/AKR1C4 Antibody (2C11) [H00001109-M01] - Analysis of monoclonal antibody to AKR1C4 on formalin-fixed paraffin-embedded human liver tissue. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize2/Aldo-keto-Reductase-1C4-AKR1C4-Antibody-(2C11)-Immunohistochemistry-Paraffin-H00001109-M01-img0013.jpg)
![SDS-Page: Recombinant Human C1q Protein [H00000712-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize2/Recombinant-Human-C1q-Protein-SDS-Page-H00000712-P01-img0002.jpg)
![Western Blot: ARHGAP4 Antibody [NBP1-88527] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.](http://images.novusbio.com/fullsize2/ARHGAP4-Antibody-Western-Blot-NBP1-88527-img0009.jpg)
![Immunohistochemistry-Paraffin: ARHGAP4 Antibody [NBP1-88527] - Staining of human spleen shows high expression.](http://images.novusbio.com/fullsize2/ARHGAP4-Antibody-Immunohistochemistry-Paraffin-NBP1-88527-img0011.jpg)
![Western Blot: ARHGAP4 Antibody [NBP1-88527] - Analysis in human cell line RPMI-8226.](http://images.novusbio.com/fullsize2/ARHGAP4-Antibody-Western-Blot-NBP1-88527-img0007.jpg)
![Western Blot: PFDN4 Antibody [NBP1-88526] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize2/PFDN4 Antibody-Western Blot-NBP1-88526-img0004.jpg)
![Immunohistochemistry-Paraffin: PFDN4 Antibody [NBP1-88526] - Staining of human tonsil shows strong cytoplasmic positivity in most cells.](http://images.novusbio.com/fullsize2/PFDN4-Antibody-Immunohistochemistry-Paraffin-NBP1-88526-img0002.jpg)
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-31378] - A. 50 ug rat liver lysate/extract 10% SDS-PAGE AKR1C3 antibody [C2C3], C-term dilution: 1:2000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-Western-Blot-NBP1-31378-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-31378] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize2/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31378-img0006.jpg)
![Immunohistochemistry-Paraffin: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-31378] - Paraffin-embedded NCI-N87 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize2/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-Immunohistochemistry-Paraffin-NBP1-31378-img0007.jpg)
![Western Blot: Vitronectin Antibody [NBP2-20866] - HepG2 whole cell extract and conditioned medium (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vitronectin antibody diluted at 1:1000.](http://images.novusbio.com/fullsize2/Vitronectin-Antibody-Western-Blot-NBP2-20866-img0007.jpg)
![Immunohistochemistry-Paraffin: Vitronectin Antibody [NBP2-20866] - Analysis of Colon carcinoma at 1:500 dilution.](http://images.novusbio.com/fullsize2/Vitronectin-Antibody-Immunohistochemistry-Paraffin-NBP2-20866-img0003.jpg)
![Western Blot: Vitronectin Antibody [NBP2-20866] - Mouse tissue extracts (50 ug) was separated by 10 % SDS-PAGE, and the membrane was blotted with Vitronectin antibody diluted by 1:1000.](http://images.novusbio.com/fullsize2/Vitronectin-Antibody-Western-Blot-NBP2-20866-img0005.jpg)
![SDS-Page: Recombinant Human Aldo-keto Reductase 1C1/AKR1C1 Protein [NBP1-41223]](http://images.novusbio.com/images2/Recombinant-Human-Aldo-keto-Reductase-1C1-AKR1C1-Protein-SDS-Page-NBP1-41223-img0002.jpg "SDS-Page: Recombinant Human Aldo-keto Reductase 1C1/AKR1C1 Protein [NBP1-41223]")
![Flow (Intracellular): Complement C3 Antibody (11H9) [NB200-540] - An intracellular stain was performed on RAW 246.7 cells with Complement C3 (11H9-3-2) antibody NB200-540 (blue) and a matched isotype control NBP2-31382 (orange). Cells were either treated with 3uM Monensin for 3 hours to block the secretion of Complement C3 (B) or grown in normal media (A). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).](http://images.novusbio.com/images2/Complement-C3-Antibody-(11H9)-Flow-(Intracellular)-NB200-540-img0005.jpg "Flow (Intracellular): Complement C3 Antibody (11H9) [NB200-540] - An intracellular stain was performed on RAW 246.7 cells with Complement C3 (11H9-3-2) antibody NB200-540 (blue) and a matched isotype control NBP2-31382 (orange). Cells were either treated with 3uM Monensin for 3 hours to block the secretion of Complement C3 (B) or grown in normal media (A). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).")
![Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) Pacific BlueTM.](http://images.novusbio.com/images2/CD59-Antibody-(MEM-43)-Flow-Cytometry-NB500-330-img0007.jpg "Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) Pacific BlueTM.")