Recombinant Human Aldo-keto Reductase 1C1/AKR1C1 Protein

SDS-Page: Recombinant Human Aldo-keto Reductase 1C1/AKR1C1 Protein [NBP2-52275] - 15% SDS Page (3 ug)

• Reactivity: Hu
• Applications: Func, PAGE, Bioactivity
• Concentration: 1 mg/ml

Recombinant Human Aldo-keto Reductase 1C1/AKR1C1 Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 1-323 of Human Aldo-keto Reductase 1C1/AKR1C1
  
  

  Source: E.coli

  Amino Acid Sequence: MDSKYQCVKL NDGHFMPVLG FGTYAPAEVP KSKALEATKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCNSH RPELVRPALE RSLKNLQLDY VDLYLIHFPV SVKPGEEVIP KDENGKILFD TVDLCATWEA VEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNQR KLLDFCKSKD IVLVAYSALG SHREEPWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTS EEMKAIDGLN RNVRYLTLDI FAGPPNYPFS DEY

• Details of Functionality: Specific activity is > 500 pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25C.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: AKR1C1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
• Endotoxin Note: < 1.0 EU per 1ug of protein (determined by LAL method)

Applications/Dilutions

• Theoretical MW: 36.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Tris-HCl buffer (pH 8.5) containing 0.1M NaCl, 20% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Alternate Names for Recombinant Human Aldo-keto Reductase 1C1/AKR1C1 Protein

• 20 alpha-hydroxysteroid dehydrogenase
• 20-ALPHA-HSD
• 20-alpha-hydroxysteroid dehydrogenase
• 2-ALPHA-HSD
• AKR1C1
• Aldo-keto Reductase 1C1
• aldo-keto reductase family 1 member C1
• aldo-keto reductase family 1, member C1 (dihydrodiol dehydrogenase 1; 20-alpha(3-alpha)-hydroxysteroid dehydrogenase)
• AldoketoReductase 1C1
• C9
• Chlordecone reductase homolog HAKRC
• DD1/DD2
• DD1MGC8954
• DDH1
• DDHH-37
• dihydrodiol dehydrogenase 1
• Dihydrodiol dehydrogenase 1/2
• dihydrodiol dehydrogenase isoform DD1
• EC 1.1.1
• EC 1.1.1.-
• EC 1.1.1.112
• EC 1.1.1.149,2-ALPHA-HSD
• EC 1.3.1.20
• HAKRC
• HAKRCDDH1aldo-keto reductase C
• HBAB
• hepatic dihydrodiol dehydrogenase
• High-affinity hepatic bile acid-binding protein
• Indanol dehydrogenase
• MBAB
• Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase
• type II 3-alpha-hydroxysteroid dehydrogenase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: AKR1C1