Rat ALDH2 ELISA Kit (Colorimetric)

Rat ALDH2 ELISA Kit (Colorimetric) [NBP2-77016] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and tissue homogenates
• Standard Curve Range: 0.312 ng/mL - 20 ng/mL
• Sensitivity: 0.078 ng/mL

Rat ALDH2 ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 1-5 hours
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat ALDH2. No significant cross-reactivity or interference between Rat ALDH2 and analogues was observed.
• Standard Curve Range: 0.312 ng/mL - 20 ng/mL
• Sensitivity: 0.078 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 10
• Intra-Assay: %CV < 8
• Spike Recovery: 86 - 96%
• Sample Volume: 50 - 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: ALDH2

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat ALDH2 ELISA Kit (Colorimetric)

• acetaldehyde dehydrogenase 2
• aldehyde dehydrogenase 2 family (mitochondrial)
• aldehyde dehydrogenase, mitochondrial
• ALDH class 2
• ALDH2
• ALDH-E2
• ALDHI
• ALDM
• EC 1.2.1
• EC 1.2.1.3
• liver mitochondrial ALDH
• MGC1806
• nucleus-encoded mitochondrial aldehyde dehydrogenase 2

Background

ALDH2 belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for ALDH2 ELISA Kit (NBP2-77016). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: ALDH2