ALDH1L1 Antibody (4A12) - BSA Free

Western Blot: ALDH1L1 Antibody (4A12) [NBP2-50045] - Analysis of rat and mouse tissue lysates using mouse ALDH1L1 mAb, dilution 1:5000 (Green): [1] protein standard (Red), rat tissue lysates: [2] heart, [3] liver, [4] kidney, [5] lung, [6] brain, and [7] spinal cord; Also mouse tissue lysates: [8] brain, and [9] spinal cord. The band at 100kDa corresponds to ALDH1L1 protein. In line with published work, ALDH1L1 is a major protein of liver and CNS tissue.

Immunocytochemistry/Immunofluorescence: ALDH1L1 Antibody (4A12) [NBP2-50045] - Analysis of mixed cortical neuron-glial cell culture from an E20 rat stained with mouse ALDH1L1 mAb, dilution 1:1,000 (Green), and costained with chicken Vimentin pAb, dilution 1:5000 (Red). DAPI staining of nuclear DNA (Blue). The ALDH1L1 antibody produces strong cytoplasmic staining of astrocytic glial cells, while the Vimentin antibody labels the intermediate filament cytoskeleton in fibroblasts and other glial cells. Some cells, presumably developing astrocytes, are stained by both ALDH1L1 and vimentin and so appear yellow.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: 4A12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ALDH1L1 Antibody (4A12) - BSA Free Summary

• Immunogen: Amino acids 1- 401 of human ALDH1L1. [UniProt# O75891]
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALDH1L1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:1000-1:2000
  • Immunohistochemistry 1:1000-1:2000
  • Western Blot 1:5000-1:10000
• Theoretical MW: 100 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50% PBS, 50% glycerol
• Preservative: 0.035% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ALDH1L1 Antibody (4A12) - BSA Free

• 10-formyltetrahydrofolate dehydrogenase
• aldehyde dehydrogenase 1 family, member L1
• aldehyde dehydrogenase family 1 member L1
• Cytosolic 10-formyltetrahydrofolate dehydrogenase
• DKFZp781N0997
• EC 1.5.1.6,10-FTHFDH
• FDH
• formyltetrahydrofolate dehydrogenase
• FTHFD10-fTHF

Background

ALDH1L1 is encoded by this gene catalyzes the conversion of 10-formyltetrahydrofolate, NADP, and water to tetrahydrofolate, NADPH, and carbon dioxide. The encoded protein belongs to the aldehyde dehydrogenase family and is responsible for formate oxidation in vivo. Deficiencies in this gene can result in an accumulation of formate and subsequent methanol poisoning.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ALDH1L1 Antibody (NBP2-50045)(3)

Publications using NBP2-50045

• Hamilton HL, Kinscherf NA, Balmer G et al. FABP7 drives an inflammatory response in human astrocytes and is upregulated in Alzheimer's disease GeroScience 2023-09-09 [PMID: 37688656] (IHC-P, Mouse)
• Marc Oria, Bedika Pathak, Zhen Li, Kenan Bakri, Kara Gouwens, Maria Florencia Varela, Kristin Lampe, Kendall P. Murphy, Chia-Ying Lin, Jose L. Peiro Premature Neural Progenitor Cell Differentiation Into Astrocytes in Retinoic Acid-Induced Spina Bifida Rat Model Frontiers in Molecular Neuroscience 2022-06-17 [PMID: 35782393] (Western Blot, Immunocytochemistry/ Immunofluorescence)
• Yoon S, Hong N, Lee M, Ahn J Photobiomodulation with a 660-Nanometer Light-Emitting Diode Promotes Cell Proliferation in Astrocyte Culture Cells 2021-07-02 [PMID: 34359834] (ICC/IF, WB)

Review for ALDH1L1 Antibody (NBP2-50045) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer ICC Human 07/27/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: human immortalized astrocyte cells
• Species: Human

Bioinformatics

• Gene Symbol: ALDH1L1
• Uniprot:
  • O75891(Human)