![Western Blot: alcohol dehydrogenase Antibody (JG82-31) [NBP2-75398] - Analysis of Alcohol Dehydrogenase on rat liver tissue lysate using anti-Alcohol Dehydrogenase antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize2/alcohol dehydrogenase Antibody (JG82-31)-Western Blot-NBP2-75398-img0005.jpg "Western Blot: alcohol dehydrogenase Antibody (JG82-31) [NBP2-75398] - Analysis of Alcohol Dehydrogenase on rat liver tissue lysate using anti-Alcohol Dehydrogenase antibody at 1/1,000 dilution.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IHC-P |
| Clone | JG82-31 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Recombinant protein within human Alcohol Dehydrogenase aa 1-200. |
| Localization | Cytoplasm. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | ADH1A |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 40 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 1%BSA, 40%Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Diseases for alcohol dehydrogenase Antibody (NBP2-75398)Discover more about diseases related to alcohol dehydrogenase Antibody (NBP2-75398).
| Pathways for alcohol dehydrogenase Antibody (NBP2-75398)View related products by pathway.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ADH1A |
![Immunocytochemistry/Immunofluorescence: alcohol dehydrogenase Antibody (JG82-31) [NBP2-75398] - Staining Alcohol Dehydrogenase in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/alcohol-dehydrogenase-Antibody-(JG82-31)-Immunocytochemistry-Immunofluorescence-NBP2-75398-img0001.jpg "Immunocytochemistry/Immunofluorescence: alcohol dehydrogenase Antibody (JG82-31) [NBP2-75398] - Staining Alcohol Dehydrogenase in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: alcohol dehydrogenase Antibody (JG82-31) [NBP2-75398] - Analysis of paraffin-embedded rat liver tissue using anti-Alcohol Dehydrogenase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/alcohol-dehydrogenase-Antibody-(JG82-31)-Immunohistochemistry-Paraffin-NBP2-75398-img0004.jpg "Immunohistochemistry-Paraffin: alcohol dehydrogenase Antibody (JG82-31) [NBP2-75398] - Analysis of paraffin-embedded rat liver tissue using anti-Alcohol Dehydrogenase antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: alcohol dehydrogenase Antibody (JG82-31) [NBP2-75398] - Analysis of paraffin-embedded human liver tissue using anti-Alcohol Dehydrogenase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/alcohol-dehydrogenase-Antibody-(JG82-31)-Immunohistochemistry-Paraffin-NBP2-75398-img0002.jpg "Immunohistochemistry-Paraffin: alcohol dehydrogenase Antibody (JG82-31) [NBP2-75398] - Analysis of paraffin-embedded human liver tissue using anti-Alcohol Dehydrogenase antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: alcohol dehydrogenase Antibody (JG82-31) [NBP2-75398] - Analysis of paraffin-embedded mouse liver tissue using anti-Alcohol Dehydrogenase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/alcohol-dehydrogenase-Antibody-(JG82-31)-Immunohistochemistry-Paraffin-NBP2-75398-img0003.jpg "Immunohistochemistry-Paraffin: alcohol dehydrogenase Antibody (JG82-31) [NBP2-75398] - Analysis of paraffin-embedded mouse liver tissue using anti-Alcohol Dehydrogenase antibody. Counter stained with hematoxylin.")
![Immunocytochemistry/Immunofluorescence: AKR1B1 Antibody [NBP1-89146] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/images2/AKR1B1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89146-img0006.jpg)
![Immunohistochemistry-Paraffin: AKR1B1 Antibody [NBP1-89146] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/images2/AKR1B1-Antibody-Immunohistochemistry-Paraffin-NBP1-89146-img0016.jpg)
![Immunohistochemistry-Paraffin: AKR1B1 Antibody [NBP1-89146] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/images2/AKR1B1-Antibody-Immunohistochemistry-Paraffin-NBP1-89146-img0010.jpg)
![Western Blot: Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) [NB100-56603] - Analysis using Azide Free version of NB100-56603. LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, cells were (A) left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 mi](http://images.novusbio.com/images2/Androgen-R-NR3C4-[p-Ser213--p-Ser210]-Antibody-(156C135.2)-Western-Blot-NB100-56603-img0004.jpg)
![Western Blot: Ferredoxin Reductase Antibody [NBP2-38530] - Analysis using Anti-FDXR antibody NBP2-38530 (A) shows similar pattern to independent antibody NBP2-38706 (B).](http://images.novusbio.com/images2/Ferredoxin-Reductase-Antibody-Western-Blot-NBP2-38530-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Ferredoxin Reductase Antibody [NBP2-38530] - Staining of human cell line U-2 OS shows positivity in mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/images2/Ferredoxin-Reductase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38530-img0001.jpg)
![Immunohistochemistry-Paraffin: Ferredoxin Reductase Antibody [NBP2-38530] - Staining in human adrenal gland and lymph node tissues using NBP2-38530 antibody. Corresponding FDXR RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/Ferredoxin-Reductase-Antibody-Immunohistochemistry-Paraffin-NBP2-38530-img0019.jpg)
![Western Blot: AKR1A1 Antibody (6E3) [NBP2-02164] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKR1A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKR1A1.](http://images.novusbio.com/images/AKR1A1 Antibody (6E3)-Western Blot-NBP2-02164-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: AKR1A1 Antibody (6E3) [NBP2-02164] Staining of COS7 cells transiently transfected by pCMV6-ENTRY AKR1A1.](http://images.novusbio.com/images/AKR1A1 Antibody (6E3)-Immunocytochemistry-Immunofluorescence-NBP2-02164-img0007.jpg)
![Immunohistochemistry-Paraffin: AKR1A1 Antibody (6E3) [NBP2-02164] - Staining of paraffin-embedded liver tissue using anti-AKR1A1 mouse monoclonal antibody.](http://images.novusbio.com/images/AKR1A1 Antibody (6E3)-Immunohistochemistry-Paraffin-NBP2-02164-img0006.jpg)
![Western Blot: Sorbitol Dehydrogenase Antibody [NBP1-87416] - Analysis using Anti-SORD antibody NBP1-87416 (A) shows similar pattern to independent antibody NBP1-87415 (B).](http://images.novusbio.com/images2/Sorbitol-Dehydrogenase-Antibody-Western-Blot-NBP1-87416-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Sorbitol Dehydrogenase Antibody [NBP1-87416] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/images2/Sorbitol-Dehydrogenase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87416-img0005.jpg)
![Immunohistochemistry-Paraffin: Sorbitol Dehydrogenase Antibody [NBP1-87416] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/images2/Sorbitol-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-87416-img0020.jpg)
-img0001.jpg)
![Western Blot: alcohol dehydrogenase 1B Antibody (OTI4F12) - Azide and BSA Free [NBP2-70092] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY alcohol dehydrogenase 1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with an](http://images.novusbio.com/images2/alcohol-dehydrogenase-1B-Antibody-(OTI4F12)---Azide-and-BSA-Free-Western-Blot-NBP2-70092-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: alcohol dehydrogenase 1B Antibody (OTI4F12) - Azide and BSA Free [NBP2-70092] - Staining of HeLa cells using anti-alcohol dehydrogenase 1B mouse monoclonal antibody.](http://images.novusbio.com/images2/alcohol-dehydrogenase-1B-Antibody-(OTI4F12)---Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70092-img0008.jpg)
![Immunohistochemistry: alcohol dehydrogenase 1B Antibody (OTI4F12) - Azide and BSA Free [NBP2-70092] - Staining of paraffin-embedded Human liver tissue using anti-alcohol dehydrogenase 1B mouse monoclonal antibody.](http://images.novusbio.com/images2/alcohol-dehydrogenase-1B-Antibody-(OTI4F12)---Azide-and-BSA-Free-Immunohistochemistry-NBP2-70092-img0014.jpg)
![Western Blot: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Probing of Western blots of pancreatic lysates from control and iron overloaded Tg40 PrP and PrP-/- mice with antibodies specific for insulin dimer and pentamer (upper and lower panels) shows decreased expression in Tg40 PrP relative to PrP-/- samples (lanes 1 & 5 vs. 3 & 7). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Western-Blot-NBP2-34260-img0006.jpg)
![Immunohistochemistry: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Immunohistochemistry of pancreas shows relatively higher reactivity for insulin in PrP-/- relative to Tg40 PrP sections (panels 1 & 3). Iron overloading decreases insulin reactivity in Tg40 PrP but not in PrP-/- samples (panels 1 vs. 2 & 3 vs. 4). Reaction for glucagon is higher in iron overloaded Tg40 PrP relative to control samples (panels 5 & 6). The difference is minimal in PrP-/- samples (panels 7 & 8). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Immunohistochemistry-NBP2-34260-img0005.jpg)
![Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)](http://images.novusbio.com/images2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Immunohistochemistry-Paraffin-NBP2-34260-img0002.jpg)
![Western Blot: Aldo-keto Reductase 1B10/AKR1B10 Antibody (1A6) [H00057016-M01] - AKR1B10 monoclonal antibody (M01), clone 1A6 Analysis of AKR1B10 expression in HepG2.](http://images.novusbio.com/images2/Aldo-keto-Reductase-1B10-AKR1B10-Antibody-(1A6)-Western-Blot-H00057016-M01-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Aldo-keto Reductase 1B10/AKR1B10 Antibody (1A6) [H00057016-M01] - Analysis of monoclonal antibody to AKR1B10 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/images2/Aldo-keto-Reductase-1B10-AKR1B10-Antibody-(1A6)-Immunocytochemistry-Immunofluorescence-H00057016-M01-img0007.jpg)
![Immunohistochemistry-Paraffin: Aldo-keto Reductase 1B10/AKR1B10 Antibody (1A6) [H00057016-M01] - Analysis of monoclonal antibody to AKR1B10 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.](http://images.novusbio.com/images2/Aldo-keto-Reductase-1B10-AKR1B10-Antibody-(1A6)-Immunohistochemistry-Paraffin-H00057016-M01-img0009.jpg)
![Western Blot: alcohol dehydrogenase 1C Antibody [NBP2-92824] - Analysis of extracts of various cell lines, using alcohol dehydrogenase 1C.Exposure time: 10s.](http://images.novusbio.com/images2/alcohol dehydrogenase 1C Antibody-Western Blot-NBP2-92824-img0003.jpg)
![Immunohistochemistry-Paraffin: alcohol dehydrogenase 1C Antibody [NBP2-92824] - Paraffin-embedded human stomach using alcohol dehydrogenase 1C.](http://images.novusbio.com/images2/alcohol-dehydrogenase-1C-Antibody-Immunohistochemistry-Paraffin-NBP2-92824-img0002.jpg)
![Immunohistochemistry-Paraffin: alcohol dehydrogenase 1C Antibody [NBP2-92824] - Paraffin-embedded human esophagus using alcohol dehydrogenase 1C.](http://images.novusbio.com/images2/alcohol-dehydrogenase-1C-Antibody-Immunohistochemistry-Paraffin-NBP2-92824-img0001.jpg)
![Western Blot: ARIH1 Antibody [NB100-1162] - (0.5ug/ml) of U937 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.](http://images.novusbio.com/images/ARIH1 Antibody-Western Blot-NB100-1162-img0002.jpg)
![Immunohistochemistry-Paraffin: ARIH1 Antibody [NB100-1162] - staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/images2/ARIH1-Antibody-Immunohistochemistry-Paraffin-NB100-1162-img0003.jpg)
![Western Blot: DCXR Antibody (7D11) [NBP2-02192] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DCXR (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DCXR.](http://images.novusbio.com/images/DCXR Antibody (7D11)-Western Blot-NBP2-02192-img0001.jpg)
![Immunohistochemistry-Paraffin: DCXR Antibody (7D11) [NBP2-02192] Staining of paraffin-embedded thyroid tissue using anti-DCXRmouse monoclonal antibody.](http://images.novusbio.com/images/DCXR Antibody (7D11)-Immunohistochemistry-Paraffin-NBP2-02192-img0007.jpg)
![Immunohistochemistry-Paraffin: DCXR Antibody (7D11) [NBP2-02192] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-DCXR mouse monoclonal antibody.](http://images.novusbio.com/images/DCXR Antibody (7D11)-Immunohistochemistry-Paraffin-NBP2-02192-img0002.jpg)
![Western Blot: Catalase Antibody [NBP2-24916] - Analysis in liver lysate of 1) human 2) mouse and 3) rat at 0.1 ug/ml.](http://images.novusbio.com/images2/Catalase-Antibody-Western-Blot-NBP2-24916-img0001.jpg)
![Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-24916] - Staining of human liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.](http://images.novusbio.com/images2/Catalase-Antibody-Immunohistochemistry-Paraffin-NBP2-24916-img0002.jpg)
![Immunohistochemistry-Paraffin: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Staining in human adrenal gland and liver tissues using anti-QPCT antibody. Corresponding QPCT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/Glutaminyl-peptide-Cyclotransferase-QPCT-Antibody-Immunohistochemistry-Paraffin-NBP1-81838-img0010.jpg)
![Western Blot: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Analysis in control (vector only transfected HEK293T lysate) and QPCT over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/images2/Glutaminyl-peptide-Cyclotransferase-QPCT-Antibody-Western-Blot-NBP1-81838-img0011.jpg)
![Western Blot: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Analysis in human cell line SK-MEL-30.](http://images.novusbio.com/images2/Glutaminyl-peptide-Cyclotransferase-QPCT-Antibody-Western-Blot-NBP1-81838-img0006.jpg)
![Immunohistochemistry-Paraffin: XPR1 Antibody [NBP2-29487] - Anti-XPR1 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/images2/XPR1-Antibody-Immunohistochemistry-Paraffin-NBP2-29487-img0004.jpg)
![Immunohistochemistry-Paraffin: XPR1 Antibody [NBP2-29487] - Analysis of anti-XPR1 antibody with spleen at 8 ug/ml.](http://images.novusbio.com/images2/XPR1-Antibody-Immunohistochemistry-Paraffin-NBP2-29487-img0002.jpg)
![Immunohistochemistry-Paraffin: XPR1 Antibody [NBP2-29487] - Analysis of anti-XPR1 antibody with human breast, carcinoma.](http://images.novusbio.com/images2/XPR1-Antibody-Immunohistochemistry-Paraffin-NBP2-29487-img0003.jpg)
![Western Blot: ST3GAL4 Antibody [NBP2-95141] - Analysis of extracts of various cell lines, using ST3GAL4. Exposure time: 60s.](http://images.novusbio.com/images2/ST3GAL4 Antibody-Western Blot-NBP2-95141-img0002.jpg)
![Immunohistochemistry-Paraffin: ST3GAL4 Antibody [NBP2-95141] - Paraffin-embedded rat kidney using ST3GAL4.](http://images.novusbio.com/images2/ST3GAL4-Antibody-Immunohistochemistry-Paraffin-NBP2-95141-img0001.jpg)
![Western Blot: Albumin Antibody [NB600-41532] - Analysis using the HRP conjugate of NB600-41532. Detection of Albumin in whole mouse liver extracts. Image courtesy of anonymous customer product review.](http://images.novusbio.com/images2/Albumin-Antibody-Western-Blot-NB600-41532-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Albumin Antibody [NB600-41532] - NLRP12 downregulates multiple JNK activating pathways in hepatocytes. For checking the purity of primary hepatocytes, hepatocytes isolated from healthy WT and Nlrp12-/- mouse livers were immunostained with antibody for hepatocyte-specific marker Albumin and counterstained with DAPI. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/40396), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Albumin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-41532-img0003.jpg)
![Western Blot: alcohol dehydrogenase 7 Antibody [NBP1-90232] - Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate](http://images.novusbio.com/images2/alcohol-dehydrogenase-7-Antibody-Western-Blot-NBP1-90232-img0007.jpg)
![Immunohistochemistry-Paraffin: alcohol dehydrogenase 7 Antibody [NBP1-90232] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/images2/alcohol-dehydrogenase-7-Antibody-Immunohistochemistry-Paraffin-NBP1-90232-img0013.jpg)
![Western Blot: alcohol dehydrogenase 7 Antibody [NBP1-90232] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue](http://images.novusbio.com/images2/alcohol-dehydrogenase-7-Antibody-Western-Blot-NBP1-90232-img0005.jpg)
![Western Blot: AKR7A2 Antibody [NBP1-32828] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/images2/AKR7A2 Antibody-Western Blot-NBP1-32828-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: AKR7A2 Antibody [NBP1-32828] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/images2/AKR7A2 Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32828-img0003.jpg)
![Immunohistochemistry-Paraffin: AKR7A2 Antibody [NBP1-32828] - AKR7A2 Antibody detects AKR7A2 protein at cytoplasm on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. AKR7A2 Antibody dilution: 1:500.](http://images.novusbio.com/images2/AKR7A2-Antibody-Immunohistochemistry-Paraffin-NBP1-32828-img0006.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")