alcohol dehydrogenase Antibody (JG82-31)

Western Blot: alcohol dehydrogenase Antibody (JG82-31) [NBP2-75398] - Analysis of Alcohol Dehydrogenase on rat liver tissue lysate using anti-Alcohol Dehydrogenase antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: alcohol dehydrogenase Antibody (JG82-31) [NBP2-75398] - Staining Alcohol Dehydrogenase in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: alcohol dehydrogenase Antibody (JG82-31) [NBP2-75398] - Analysis of paraffin-embedded rat liver tissue using anti-Alcohol Dehydrogenase antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: alcohol dehydrogenase Antibody (JG82-31) [NBP2-75398] - Analysis of paraffin-embedded human liver tissue using anti-Alcohol Dehydrogenase antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: alcohol dehydrogenase Antibody (JG82-31) [NBP2-75398] - Analysis of paraffin-embedded mouse liver tissue using anti-Alcohol Dehydrogenase antibody. Counter stained with hematoxylin.

Product Discontinued

alcohol dehydrogenase Antibody (JG82-31) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human alcohol dehydrogenase aa 2-107 / 375. (SwissProt: P07327 Human; SwissProt: P00329 Mouse; SwissProt: P06757 Rat)
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ADH1A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for alcohol dehydrogenase Antibody (JG82-31)

• ADH, alpha subunit
• ADH1alcohol dehydrogenase 1A
• alcohol dehydrogenase 1 (class I), alpha polypeptide
• alcohol dehydrogenase 1A (class I), alpha polypeptide
• Alcohol dehydrogenase subunit alpha
• aldehyde reductase
• EC 1.1.1
• EC 1.1.1.1

Background

ADH (alcohol dehydrogenase) family of proteins metabolize a variety of substances such as ethanol, retinal, other aliphatic alcohol, hydroxysteroids, and lipid peroxidation products. With the coenzyme NAD, ADH catalyzes the reversible conversion of organic alcohol to ketones or aldehydes. ADH play a major role in ethanol metabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ADH1A