Recombinant Human alcohol dehydrogenase 1B GST (N-Term) Protein

SDS-Page: Recombinant Human alcohol dehydrogenase 1B Protein [H00000125-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.

• Reactivity: Hu
• Applications: WB, ELISA, MA, AP, Enzyme Activity

Recombinant Human alcohol dehydrogenase 1B GST (N-Term) Protein Summary

• Description: A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-375 of Human ADH1B
  
  

  Source: Wheat Germ (in vitro)

  Amino Acid Sequence: MSTAGKVIKCKAAVLWEVKKPFSIEDVEVAPPKAYEVRIKMVAVGICRTDDHVVSGNLVTPLPVILGHEAAGIVESVGEGVTTVKPGDKVIPLFTPQCGKCRVCKNPESNYCLKNDLGNPRGTLQDGTRRFTCRGKPIHHFLGTSTFSQYTVVDENAVAKIDAASPLEKVCLIGCGFSTGYGSAVNVAKVTPGSTCAVFGLGGVGLSAVMGCKAAGAARIIAVDINKDKFAKAKELGATECINPQDYKKPIQEVLKEMTDGGVDFSFEVIGRLDTMMASLLCCHEACGTSVIVGVPPASQNLSINPMLLLTGRTWKGAVYGGFKSKEGIPKLVADFMAKKFSLDALITHVLPFEKINEGFDLLHSGKSIRTVLTF

• Preparation Method: in vitro wheat germ expression system
• Details of Functionality: This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
• Source: Wheat germ
• Protein/Peptide Type: Recombinant Protein
• Gene: ADH1B
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • ELISA
  • Enzyme Activity
  • Immunoaffinity Purification
  • Protein Array
  • Western Blot
• Theoretical MW: 66.99 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human alcohol dehydrogenase 1B GST (N-Term) Protein

• ADH, beta subunit
• ADH2alcohol dehydrogenase 1B
• alcohol dehydrogenase 1B (class I), beta polypeptide
• alcohol dehydrogenase 2 (class I), beta polypeptide
• Alcohol dehydrogenase subunit beta
• aldehyde reductase
• DKFZp686C06125
• EC 1.1.1
• EC 1.1.1.1

Background

ADH1B( AAH33009, 1 a.a. - 376 a.a.) recombinant protein with GST.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for alcohol dehydrogenase 1B Recombinant Protein (H00000125-P01)(1)

Publication using H00000125-P01

• Atsriku C, Hoffmann M, Moghaddam M et al. In vitro metabolism of a novel JNK inhibitor tanzisertib: interspecies differences in oxido-reduction and characterization of enzymes involved in metabolism. Xenobiotica 2014-12-05 [PMID: 25475995]

Bioinformatics

• Gene Symbol: ADH1B