AKAP10 Antibody

Western Blot: AKAP10 Antibody [NB100-94882] - Analysis of AKAP10 using this antibody at 1 ug/ml in Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Rt, Hu, Mu, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

AKAP10 Antibody Summary

• Description: Novus Biologicals Goat AKAP10 Antibody (NB100-94882) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-RKVKGKEQEKTSD corresponding to internal region according to NP_009133.2.
• Epitope: C-RKVKGKEQEKTSD
• Predicted Species: Human (100%), Mouse (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AKAP10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 74 kDa band observed in rat brain lysates (calculated MW of 73.8 kDa band according to human NP_009133.2 and according to rat NP_001108078.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for AKAP10 Antibody

• A kinase (PRKA) anchor protein 10
• AKAP-10
• A-kinase anchor protein 10, mitochondrial
• D-AKAP2
• D-AKAP-2
• Dual specificity A kinase-anchoring protein 2
• dual-specificity A-kinase anchoring protein 2
• MGC9414
• mitochondrial A kinase PPKA anchor protein 10
• PRKA10a kinase anchor protein 10, mitochondrial
• protein kinase A anchoring protein 10
• Protein kinase A-anchoring protein 10

Background

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein interacts with both the type I and type II regulatory subunits of PKA; therefore, it is a dual-specific AKAP. This protein is highly enriched in mitochondria. It contains RGS (regulator of G protein signalling) domains, in addition to a PKA-RII subunit-binding domain. The mitochondrial localization and the presence of RGS domains may have important implications for the function of this protein in PKA and G protein signal transduction. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AKAP10
• Entrez:
  • 11216(Human)
  • 56697(Mouse)
  • 360540(Rat)
• Uniprot:
  • NP_009133.2(Human)