AKAP1 Antibody

Images

 
Western Blot: AKAP1 Antibody [NBP2-15319] - Wild-type (WT) and AKAP1 knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKAP1 antibody diluted at 1:2000. ...read more
Immunocytochemistry/ Immunofluorescence: AKAP1 Antibody [NBP2-15319] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: AKAP1 Antibody [NBP2-15319] - Paraffin-embedded Colon ca, using antibody at 1:250 dilution.
Western Blot: AKAP1 Antibody [NBP2-15319] - Sample (30 ug of whole cell lysate) A: HeLa 7. 5% SDS PAGE; antibody diluted at 1:10000.

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

AKAP1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human AKAP1. The exact sequence is proprietary.
Localization
Mitochondrion outer membrane
Predicted Species
Chimpanzee (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AKAP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
AKAP1 Knockout 293T Cell Lysate

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rhesus Macaque (85%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for AKAP1 Antibody

  • A kinase (PRKA) anchor protein 1
  • a kinase anchor protein 1, mitochondrial
  • AKAP121
  • AKAP149AKAP 149
  • AKAP84
  • A-kinase anchor protein 1, mitochondrial
  • A-kinase anchor protein 149 kDa
  • A-kinase anchor protein, 149kD
  • D-AKAP1
  • D-AKAP-1
  • Dual specificity A-kinase-anchoring protein 1
  • dual-specificity A-kinase anchoring protein 1
  • MGC1807
  • PRKA1
  • protein kinase A anchoring protein 1
  • protein kinase A1
  • Protein kinase A-anchoring protein 1
  • protein kinase anchoring protein 1
  • S-AKAP84
  • SAKAP84AKAP
  • Spermatid A-kinase anchor protein 84

Background

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to type I and type II regulatory subunits of PKA and anchors them to the mitochondrion. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AKAP1
Uniprot