AKAP1 Antibody

Western Blot: AKAP1 Antibody [NBP2-15319] - Sample (30 ug of whole cell lysate) A: HeLa 7. 5% SDS PAGE; antibody diluted at 1:10000.

Immunocytochemistry/Immunofluorescence: AKAP1 Antibody [NBP2-15319] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: AKAP1 Antibody [NBP2-15319] - Human colon carcinoma, using AKAP1 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: AKAP1 Antibody [NBP2-15319] - Wild-type (WT) and AKAP1 knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKAP1 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Pm
• Applications: WB, ICC/IF, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

AKAP1 Antibody Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit AKAP1 Antibody (NBP2-15319) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human AKAP1. The exact sequence is proprietary.
• Localization: Mitochondrion outer membrane
• Predicted Species: Chimpanzee (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AKAP1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:20000
• Theoretical MW: 97 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rhesus Macaque (85%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for AKAP1 Antibody

• A kinase (PRKA) anchor protein 1
• a kinase anchor protein 1, mitochondrial
• AKAP121
• AKAP149AKAP 149
• AKAP84
• A-kinase anchor protein 1, mitochondrial
• A-kinase anchor protein 149 kDa
• A-kinase anchor protein, 149kD
• D-AKAP1
• D-AKAP-1
• Dual specificity A-kinase-anchoring protein 1
• dual-specificity A-kinase anchoring protein 1
• MGC1807
• PRKA1
• protein kinase A anchoring protein 1
• protein kinase A1
• Protein kinase A-anchoring protein 1
• protein kinase anchoring protein 1
• S-AKAP84
• SAKAP84AKAP
• Spermatid A-kinase anchor protein 84

Background

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to type I and type II regulatory subunits of PKA and anchors them to the mitochondrion. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: AKAP1
• Uniprot:
  • Q92667(Human)