AKAP10 Antibody (8C10) - Azide and BSA Free

Western Blot: AKAP10 Antibody (8C10) [H00011216-M04] - Analysis of AKAP10 expression in Raw 264.7.

Immunohistochemistry-Paraffin: AKAP10 Antibody (8C10) [H00011216-M04] - Analysis of monoclonal antibody to AKAP10 on formalin-fixed paraffin-embedded human testis. Antibody concentration 0.5 ug/ml

Western Blot: AKAP10 Antibody (8C10) [H00011216-M04] - Western blot analysis of AKAP10 over-expressed 293 cell line. Lane 2 cotransfected with AKAP10 Validated Chimera RNAi Lane 1 non-transfected control. Blot probed with H00011216-M04. GAPDH (36.1 kDa) used as loading control.

Western Blot: AKAP10 Antibody (8C10) [H00011216-M04] - Analysis of AKAP10 expression in transfected 293T cell line by AKAP10 monoclonal antibody (M04), clone 8C10. Lane 1: AKAP10 transfected lysatE (73.8 KDa). Lane 2: Non-transfected lysate.

Sandwich ELISA: AKAP10 Antibody (8C10) [H00011216-M04] - Detection limit for recombinant GST tagged AKAP10 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC, KD
• Clone: 8C10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

AKAP10 Antibody (8C10) - Azide and BSA Free Summary

• Immunogen: AKAP10 (NP_009133, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MRGAGPSPRQSPRTLRPDPGPAMSFFRRKVKGKEQEKTSDVKSIKASISVHSPQKSTKNHALLEAAGPSHVAINAISANMDSFSSSRTATLKKQPSHMEA
• Specificity: AKAP10 - A kinase (PRKA) anchor protein 10
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: AKAP10
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin), RNAi validation, and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for AKAP10 Antibody (8C10) - Azide and BSA Free

• A kinase (PRKA) anchor protein 10
• AKAP-10
• A-kinase anchor protein 10, mitochondrial
• D-AKAP2
• D-AKAP-2
• Dual specificity A kinase-anchoring protein 2
• dual-specificity A-kinase anchoring protein 2
• MGC9414
• mitochondrial A kinase PPKA anchor protein 10
• PRKA10a kinase anchor protein 10, mitochondrial
• protein kinase A anchoring protein 10
• Protein kinase A-anchoring protein 10

Background

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein interacts with both the type I and type II regulatory subunits of PKA; therefore, it is a dual-specific AKAP. This protein is highly enriched in mitochondria. It contains RGS (regulator of G protein signalling) domains, in addition to a PKA-RII subunit-binding domain. The mitochondrial localization and the presence of RGS domains may have important implications for the function of this protein in PKA and G protein signal transduction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AKAP10 Antibody (H00011216-M04)(2)

Publications using H00011216-M04

• Rinzhin S, Chase F, Karni M et al. Mitochondrial A-kinase anchoring proteins in cardiac ventricular myocytes. Physiol Rep. 2021-09-15 [PMID: 34514737]
• Eggers CT, Schafer JC, Goldenring JR et al. D-AKAP2 interacts with Rab4 and Rab11 through its RGS domains and regulates transferrin receptor recycling. J Biol Chem 284(47):32869-80. 2009-11-20 [PMID: 19797056]

Bioinformatics

• Gene Symbol: AKAP10
• Entrez:
  • 11216(Human)
• OMIM:
  • 152430(Human)
• Uniprot:
  • NP_009133(Human)