AGL Antibody (PSH04-56)

Immunohistochemistry: AGL Antibody (PSH04-56) [NBP3-32009] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-AGL antibody (NBP3-32009) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32009) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: AGL Antibody (PSH04-56) [NBP3-32009] - Western blot analysis of AGL on different lysates with Rabbit anti-AGL antibody (NBP3-32009) at 1/1,000 dilution. Lane 1: K-562 cell lysate (20 ug/Lane) Lane 2: HEK-293 cell lysate (20 ug/Lane) Lane 3: Mouse skeletal muscle tissue lysate (10 ug/Lane) Lane 4: Rat skeletal muscle tissue lysate (10 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 175 kDa Observed band size: 150 kDa Exposure time: Lane 1-2: 3 minutes; Lane 3-4: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32009) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: PSH04-56
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

AGL Antibody (PSH04-56) Summary

• Description: Novus Biologicals Rabbit AGL Antibody (PSH04-56) (NBP3-32009) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within human AGL aa 783-1,532 / 1,532. (Uniprot: P35573)
• Localization: Cytoplasm.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: AGL
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000
  • Western Blot 1:1000
• Theoretical MW: 175 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for AGL Antibody (PSH04-56)

• amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase
• GDEamylo-1, 6-glucosidase, 4-alpha-glucanotransferase
• Glycogen debrancher
• glycogen debranching enzyme

Background

AGL encodes the glycogen debrancher enzyme which is involved in glycogen degradation. This enzyme has two independent catalytic activities which occur at different sites on the protein: a 4-alpha-glucotransferase activity and a amylo-1,6-glucosidase activity. Mutations in this gene are associated with glycogen storage disease although a wide range of enzymatic and clinical variability occurs which may be due to tissue-specific alternative splicing. Alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AGL