AF9 Antibody

Western Blot: AF9 Antibody [NB100-1566] - Detection of Human AF9 on HeLa whole cell lysates using NB100-1566. AF9 was also immunoprecipitated using rabbit anti-AF9 antibodies NB100-1564, NB100-1565 and another AF9 Ab.

Product Discontinued

AF9 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 525 and the C-terminus (residue 568) of human ALL1 Fused Gene from Chromosome 9 Protein using the numbering given in entry NP_004520.1 (GeneID 4300).
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MLLT3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1-4 ug/mg
  • Western Blot 1:5000-1:15000
• Control:

  AF9 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for AF9 Antibody

• AF-9
• AF9myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog);translocated to, 3
• ALL1-fused gene from chromosome 9 protein
• myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog); translocatedto, 3
• myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 3
• Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein
• protein AF-9
• YEATS domain-containing protein 3
• YEATS3FLJ2035

Background

A human leukemogenic protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AF9 Antibody (NB100-1566)(3)

Publications using NB100-1566

• Qiao Y, Wang X, Wang R et al. AF9 promotes hESC neural differentiation through recruiting TET2 to neurodevelopmental gene loci for methylcytosine hydroxylation. Cell Discov 2015-07-28 [PMID: 27462416] (IP)
• Malik B, Hemenway CS. CBX8, a component of the Polycomb PRC1 complex, modulates DOT1L-mediated gene expression through AF9/MLLT3. FEBS Lett 2013-07-25 [PMID: 23891621] (WB, Human)
• Lin JJ, Hemenway CS. Hsp90 directly modulates the spatial distribution of AF9/MLLT3 and affects target gene expression. J Biol Chem;285(16):11966-73. 2010-04-16 [PMID: 20159978]

Bioinformatics

• Gene Symbol: MLLT3
• Entrez:
  • 4300(Human)
• Uniprot:
  • P42568(Human)