ADNP Antibody [Alexa Fluor® 700]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 700

ADNP Antibody [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human ADNP
  Leu941-Ala1102
  Accession # Q9H2P0
• Specificity: Detects human ADNP in direct ELISAs. In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse ADNP is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ADNP Antibody [Alexa Fluor® 700]

• Activity-dependent neuroprotective protein
• activity-dependent neuroprotector homeobox
• activity-dependent neuroprotector
• ADNP homeobox 1
• ADNP
• ADNP1
• ADNP1KIAA0784activity-dependent neuroprotector homeobox protein

Background

ADNP (Activity-dependent neuroprotective protein-1) is a member of a small family of VIP-induced neurotrophic factors. Although its predicted MW is 124 kDa, it runs anomalously at 150 kDa in SDS-PAGE. ADNP is known to be expressed by endothelium, astrocytes, neurons, macrophages, monocytes and B cells, and serves to down‑regulate inflammation in immune cells, while acting to promote neuronal survival in response to stress or injury. Human ADNP is 1102 amino acids (aa) in length. It has no definitive signal sequence, but is reported to be found both extracellularly and intracellularly, where it shuttles between cytoplasm and nucleus. Full-length ADNP contains nine consecutive C2H2-type zinc finger regions (aa 74-686), followed by a DNA-binding homeobox domain (aa 754-814). There are 12 utilized phosphoserine sites, two C-terminal acetylated lysines, an NLS (aa 716-733), an NES (aa 788-804) and one NAPVSIPQ (NAP) motif (aa 354-361) that likely mediates the ADNP neuroprotective function. Over aa 941-1102, human ADNP shares 93% aa identity with mouse ADNP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.