Human Adenylate Cyclase 1 ELISA Kit (Chemiluminescence)

ELISA: Human Adenylate Cyclase 1 ELISA Kit (Chemiluminescence) [NBP2-66448] - Samples were spiked with high concentrations of Human Adenylate Cyclase 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Adenylate Cyclase 1 ELISA Kit (Chemiluminescence) [NBP2-66448] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.16-10 ng/mL
• Sensitivity: 0.09 ng/mL

Human Adenylate Cyclase 1 ELISA Kit (Chemiluminescence) Summary

• Specificity: This kit recognizes Human ADCY1 in samples. No significant cross-reactivity or interference between Human ADCY1 and analogues was observed.
• Standard Curve Range: 0.16-10 ng/mL
• Sensitivity: 0.09 ng/mL
• Assay Type: Sandwich-CLIA
• Inter-Assay: CV% < 9.39%
• Intra-Assay: CV% < 10.36%
• Spike Recovery: 88-111%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Chemiluminescence)
• Gene: ADCY1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Adenylate Cyclase 1 ELISA Kit (Chemiluminescence)

• 3'-5'-cyclic AMP synthetase
• AC1
• ADCY1
• adenyl cyclase
• adenylate cyclase 1 (brain)
• Adenylate Cyclase 1
• adenylate cyclase type 1
• Adenylate cyclase type I
• Adenylyl cyclase 1
• ATP pyrophosphate-lyase 1
• Ca(2+)/calmodulin-activated adenylyl cyclase
• EC 4.6.1.1

Background

Adenylyl cyclases function to convert ATP to cyclic AMP in response to activation by a variety of hormones, neurotransmitters and other regulatory molecules. Cyclic AMP, in turn, activates several other target molecules to control a broad range of diverse phenomena such as metabolism, gene transcription and memory. Adenylyl cyclases respond to receptor-initiated signals, mediated by the Gs and Gi heterotrimeric G proteins. The binding of an agonist to a Gs-coupled receptor catalyzes the exchange of GDP (bound to Galpha s) for GTP, the dissociation of GTP-Galpha s from Gbetagamma and Galpha s)-mediated activation of adenylyl cyclase. Adenylyl cyclases type I (AC I) and III (AC III) have distinct staining within the cell nucleus of rat brain sensory neurons. AC I is expressed in myenteric ganglia as two bands of 160 kDa and 185 kDa by SDS-PAGE. Ca2+ stimulation of AC I and AC III is mediated by calmodulin. Protein associated with Myc (PAM) is a very potent inhibitor of AC I. A decrease in endogenous PAM levels in HeLa cells modulate both basal and agonist stimulated cAMP accumulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Adenylate Cyclase 1 ELISA Kit (NBP2-66448). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: ADCY1