Adenylate Cyclase 1 Antibody

Product Discontinued

Adenylate Cyclase 1 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 230-280 of Human ADCY 1.
• Specificity: A Cyclase I (D252) detects endogenous levels of A Cyclase I protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ADCY1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of A Cyclase I (D252) pAb in extracts from COLO205 cells.
  Immunohistochemistry (IHC) analyzes of A Cyclase I (D252) pAb in paraffin-embedded human brain tissue. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 25937212)
• Theoretical MW: 130 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Adenylate Cyclase 1 Antibody

• 3'-5'-cyclic AMP synthetase
• AC1
• ADCY1
• adenyl cyclase
• adenylate cyclase 1 (brain)
• Adenylate Cyclase 1
• adenylate cyclase type 1
• Adenylate cyclase type I
• Adenylyl cyclase 1
• ATP pyrophosphate-lyase 1
• Ca(2+)/calmodulin-activated adenylyl cyclase
• EC 4.6.1.1

Background

Adenylyl cyclases function to convert ATP to cyclic AMP in response to activation by a variety of hormones, neurotransmitters and other regulatory molecules. Cyclic AMP, in turn, activates several other target molecules to control a broad range of diverse phenomena such as metabolism, gene transcription and memory. Adenylyl cyclases respond to receptor-initiated signals, mediated by the Gs and Gi heterotrimeric G proteins. The binding of an agonist to a Gs-coupled receptor catalyzes the exchange of GDP (bound to Galpha s) for GTP, the dissociation of GTP-Galpha s from Gbetagamma and Galpha s)-mediated activation of adenylyl cyclase. Adenylyl cyclases type I (AC I) and III (AC III) have distinct staining within the cell nucleus of rat brain sensory neurons. AC I is expressed in myenteric ganglia as two bands of 160 kDa and 185 kDa by SDS-PAGE. Ca2+ stimulation of AC I and AC III is mediated by calmodulin. Protein associated with Myc (PAM) is a very potent inhibitor of AC I. A decrease in endogenous PAM levels in HeLa cells modulate both basal and agonist stimulated cAMP accumulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Adenylate Cyclase 1 Antibody (NBP1-19628)(1)

Publication using NBP1-19628

• Cheng Q, Yakel JL. Activation of alpha7 nicotinic acetylcholine receptors increases intracellular cAMP levels via activation of AC1 in hippocampal neurons. Neuropharmacology. [PMID: 25937212] (ICC/IF, Mouse)
  
  Details:
  Citation using the DyLight 633 form of this antibody.

Bioinformatics

• Gene Symbol: ADCY1
• Entrez:
  • 107(Human)
  • 432530(Mouse)