ADAM8 Antibody

Western Blot: Goat Polyclonal ADAM8 Antibody [NB100-1017] - (1µg/ml) staining of Caco-2 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

Western Blot: Goat Polyclonal ADAM8 Antibody [NB100-1017] - (1µg/ml) staining of Human Duodenum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Flow Cytometry: Goat Polyclonal ADAM8 Antibody [NB100-1017] - Flow cytometric analysis of paraformaldehyde fixed Caco-2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunohistochemistry-Paraffin: Goat Polyclonal ADAM8 Antibody [NB100-1017] - (6µg/ml) staining of paraffin embedded Human Lymph Node. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Control: Goat Polyclonal ADAM8 Antibody [NB100-1017] - Negative Control showing staining of paraffin embedded Human Lymph Node, with no primary antibody.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ADAM8 Antibody Summary

• Description: Novus Biologicals Goat ADAM8 Antibody (NB100-1017) is a polyclonal antibody validated for use in IHC, WB, ELISA and Flow. Anti-ADAM8 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-QRKQGAGAPTAP corresponding to C-Terminus according to NP_001100.3, NP_001157962.1.
• Localization: Cell Membrane
• Specificity: This antibody is expected to recognise isoform 1 and 3 (NP_001100.3; NP_001157962.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ADAM8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Detection limit 1:16000
  • Flow Cytometry 10 ug/ml
  • Immunohistochemistry 6-8 ug/ml
  • Immunohistochemistry-Paraffin 6-8 ug/ml
  • Western Blot 1 - 3 ug/ml
• Application Notes: Approx. 90kDa band observed in lysates of cell line Caco-2, approx. 80kDa in Human Duodenum lysates and approx. 85kDa in preliminary testing of MCF7 cell lysate (calculated MW of 88.8kDa according to NP_001100.3 and 78.8kDa according to NP_001157962.1).
• Theoretical MW: 89 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Brain Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ADAM8 Antibody

• a disintegrin and metalloproteinase domain 8
• ADAM 8
• ADAM metallopeptidase domain 8
• ADAM8
• CD156a antigen
• CD156a
• CD156human leukocyte differentiation antigen
• Cell surface antigen MS2
• EC 3.4.24
• EC 3.4.24.-
• MGC134985
• MS2
• MS2disintegrin and metalloproteinase domain-containing protein 8

Background

Members of ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. The extracellular region of ADAM8 shows significant amino acid sequence homology to hemorrhagic snake venom proteins, including the metalloprotease and disintegrin domains. The expression of ADAM8 is upregulated by retinoic acid and vitamin D3. ADAM8 is involved in protein processing at the cell surface and is also thought to act as a leukocyte differentiation marker.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ADAM8 Antibody (NB100-1017)(1)

Publication using NB100-1017

• Yoshiyama K, Higuchi Y, Kataoka M et al. CD156 (human ADAM8): expression, primary amino acid sequence, and gene location. Genomics 1997-04-01 [PMID: 9126482]

Bioinformatics

• Gene Symbol: ADAM8
• Uniprot:
  • P78325()