Mouse ADAM33 ELISA Kit (Colorimetric)

Mouse ADAM33 ELISA Kit (Colorimetric) [NBP2-76998] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and tissue homogenates
• Standard Curve Range: 15.6 pg/mL - 1000 pg/mL
• Sensitivity: 3.9 pg/mL

Mouse ADAM33 ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 1-5 hours
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse ADAM33. No significant cross-reactivity or interference between Mouse ADAM33 and analogues was observed.
• Standard Curve Range: 15.6 pg/mL - 1000 pg/mL
• Sensitivity: 3.9 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 10
• Intra-Assay: %CV < 8
• Spike Recovery: 85 - 96%
• Sample Volume: 50 - 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: ADAM33

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse ADAM33 ELISA Kit (Colorimetric)

• a disintegrin and metalloprotease 33
• a disintegrin and metalloproteinase domain 33
• ADAM 33
• ADAM metallopeptidase domain 33
• ADAM33
• C20orf153
• chromosome 20 open reading frame 153
• disintegrin and metalloproteinase domain-containing protein 33
• disintegrin and reprolysin metalloproteinase family protein
• dJ964F7.1
• DKFZp434K0521
• EC 3.4.24.-
• FLJ35308
• FLJ36751
• MGC149823
• MGC71889

Background

ADAM33 encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This protein is a type I transmembrane protein implicated in asthma and bronchial hyperresponsiveness. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for ADAM33 ELISA Kit (NBP2-76998). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: ADAM33