ADAM33 Antibody

Western Blot: ADAM33 Antibody [NB100-1787] - Analysis of ADAM33 in Human Prostate lysate using this antibody at (0.3ug/ml) with (B) and without (A) blocking with the immunizing peptide.

Western Blot: ADAM33 Antibody [NB100-1787] - (0.3ug/ml) staining of Human Prostate lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Product Discontinued

ADAM33 Antibody Summary

• Immunogen: Peptide with sequence CQSRRCRKNAFQEL corresponding to internal region according to NP_079496.1.
• Specificity: This antibody is expected to recognise isoform alpha (NP_079496.1) and not isoform beta (NP_694882.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ADAM33
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Experiments gave bands at approx. 80-90 kDa band and strong ladder from 26 to 38 kDa band in human prostate lysates after 0.3ug/ml antibody staining. The observation of multiple bands and the 38 kDa band correspond to earlier findings in scientific literature with different antibodies (PMID: 14742294). This protein has a calculated MW of 87.7 kDa band according to NP_079496.1.
• Control:

  Human Prostate Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ADAM33 Antibody

• a disintegrin and metalloprotease 33
• a disintegrin and metalloproteinase domain 33
• ADAM 33
• ADAM metallopeptidase domain 33
• ADAM33
• C20orf153
• chromosome 20 open reading frame 153
• disintegrin and metalloproteinase domain-containing protein 33
• disintegrin and reprolysin metalloproteinase family protein
• dJ964F7.1
• DKFZp434K0521
• EC 3.4.24.-
• FLJ35308
• FLJ36751
• MGC149823
• MGC71889

Background

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This protein is a type I transmembrane protein implicated in asthma and bronchial hyperresponsiveness. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ADAM33 Antibody (NB100-1787)(2)

Publications using NB100-1787

• Blakey J, Halapi E, Bjornsdottir US et al. Contribution of ADAM33 polymorphisms to the population risk of asthma. Thorax 2005-04-01 [PMID: 15790980]
• Powell RM, Wicks J, Holloway JW et al. The splicing and fate of ADAM33 transcripts in primary human airways fibroblasts. Am J Respir Cell Mol Biol 2004-07-01 [PMID: 14742294]

Bioinformatics

• Gene Symbol: ADAM33
• Entrez:
  • 80332(Human)
• Uniprot:
  • NP_079496.1(Human)