ACCN2 Antibody

Western Blot: ACCN2 Antibody [NBP2-42507] - Analysis of Mouse fetal Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Mu, Hu, Rt, Po, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ACCN2 Antibody Summary

• Description: Novus Biologicals Goat ACCN2 Antibody (NBP2-42507) is a polyclonal antibody validated for use in WB and ELISA. Anti-ACCN2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-NILPHHPARGT corresponding to C-Terminus according to NP_001086.2, NP_001243759.1.
• Specificity: This antibody is expected to recognize all reported isoforms (NP_064423.2; NP_001086.2; NP_001243759.1).
• Predicted Species: Human (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ASIC1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 70 kDa band observed in mouse fetal brain lysates (calculated MW of 64.8 kDa band according to NP_064423.2).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ACCN2 Antibody

• ACCN2
• Acid-sensing ion channel 1
• acid-sensing ion channel 1a protein
• amiloride-sensitive cation channel 2, neuronal
• ASIC
• ASIC1
• ASIC1ASIC1A
• BNaC2
• BNaC2ACCN2 variant 3
• Brain sodium channel 2
• Cation channel, amiloride-sensitive, neuronal, 2
• hBNaC2

Background

ACCN2 encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene is expressed in most if not all brain neurons, and it may be an ion channel subunit; however, its function as an ion channel remains unknown.; FUNCTION: Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Ca(2+), Li(+) and K(+).; SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Note=Localizes in synaptosomes at dendritic synapses of neurons.; TISSUE SPECIFICITY: Expressed in dorsal root ganglia (DRG) and sciatic nerve (at protein level). Widely distributed throughout the brain. Expressed in olfactory bulb, neo and allocortical regions, dentate granule cells, pyramidal cells of CA1-CA3 subfields of the hippocampal formation, habenula, basolateral amygdaloid nuclei, and in the Purkinje and granule cells of the cerebellum. Diffusely detected over most other regions of the basal ganglia, including thalamic nuclei, substantia nigra, striatum and globus pallidus, hypothalamus, midbrain, pons, medulla and choroid plexus. Isoform 3 is expressed only in dorsal root ganglion while isoform 1 is expressed in DRG, spinal chord, trigeminal ganglia and the trigeminal mesencephalic nucleus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ACCN2 Antibody (NBP2-42507)(1)

Publication using NBP2-42507

• Hoagland EN, Sherwood TW, Lee KG et al. Identification of a calcium permeable human acid-sensing ion channel 1 transcript variant. J Biol Chem 2010-12-01 [PMID: 21036899]

Bioinformatics

• Gene Symbol: ASIC1
• Entrez:
  • 41(Human)