Amiloride-sensitive cation channel 3 Antibody

• Reactivity: Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Amiloride-sensitive cation channel 3 Antibody Summary

• Immunogen: A synthetic peptide from the amino acid region 50-100 of the extracellular domain of rat Amiloride-sensitive cation channel 3 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ASIC3
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:300-1:2000
  • Immunohistochemistry-Frozen 1:300-1:2000
  • Immunohistochemistry-Paraffin 1:300-1:2000
  • Western Blot 1:300-1:2000
• Application Notes: IHC, WB, ICCIF reactivity reported in (PMID: 23095108). Amiloride-sensitive cation channel 3 antibody validated for IHC-FR from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from whole antisera
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Alternate Names for Amiloride-sensitive cation channel 3 Antibody

• ACCN3
• Acid-sensing ion channel 3
• Amiloride-sensitive cation channel 3
• amiloride-sensitive cation channel 3, testis
• ASIC3
• ASIC3hTNaC1
• DRASIC
• hASIC3
• modulatory subunit of ASIC2a
• Neuronal amiloride-sensitive cation channel 3
• proton-gated cation channel subunit
• SLNAC1
• Testis sodium channel 1
• TNAC1

Background

This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, two hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene is an acid sensor and may play an important role in the detection of lasting pH changes. In addition, a heteromeric association between this member and ACCN1 has been observed as proton-gated channels sensitive to gadolinium. Alternative splicing of this gene generates three transcript variants encoding distinct isoforms. FUNCTION: Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Generates a biphasic current with a fast inactivating and a slow sustained phase. In sensory neurons is proposed to mediate the pain induced by acidosis that occurs in ischemic, damaged or inflamed tissue. May be involved in hyperalgesia. May play a role in mechanoreception. Heteromeric channel assembly seems to modulate channel properties. SUBUNIT: Homotetramer or heterotetramer with other ASIC proteins (Probable). Interacts with STOM and DLG4. Interacts with LIN7B, MAGI1/BAIAP1, GOPC and ACCN1. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cytoplasm. Note=Cell surface expression may be stabilized by interaction with LIN7B and cytoplasmic retention by interaction with DLG4. In part cytoplasmic in cochlea cells. TISSUE SPECIFICITY: Expressed by sensory neurons. Strongly expressed in brain, spinal chord, lung, lymph nodes, kidney, pituitary, heart and testis. DEVELOPMENTAL STAGE: Expressed in fetal tissues, expression increases in lung and kidney adult tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Amiloride-sensitive cation channel 3 Antibody (NBP1-46288)(3)

Publications using NBP1-46288

• Li Z, Cai F, Tang J et al. Oxygen metabolism-balanced engineered hydrogel microspheres promote the regeneration of the nucleus pulposus by inhibiting acid-sensitive complexes Bioactive Materials 2023-06-01 [PMID: 36632505] (IHC-P, Rat)
• Montalbetti N, Rooney JG, Marciszyn AL, Carattino MD. ASIC3 fine-tunes bladder sensory signaling. Am. J. Physiol. Renal Physiol. 2018-03-21 [PMID: 29561183] (Mouse)
• Durham PL, Masterson CG. Two Mechanisms Involved in Trigeminal CGRP Release: Implications for Migraine Treatment Headache 2012-09-05 [PMID: 23095108] (ICC/IF, WB, IF/IHC, Rat)

Review for Amiloride-sensitive cation channel 3 Antibody (NBP1-46288) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Verified Customer IHC-Fr Rat 12/23/2014

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: See PMID 23095108
• Species: Rat

Bioinformatics

• Gene Symbol: ASIC3
• Entrez:
  • 9311(Human)