ABHD4 Antibody

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Immunohistochemistry-Paraffin: ABHD4 Antibody [NBP1-06009] - (3.75ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, CaSpecies Glossary
Applications ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

ABHD4 Antibody Summary

Immunogen
Peptide with sequence C-RPTNPSEIRAP corresponding to internal region according to NP_071343.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ABHD4
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:4000
Application Notes
WB: Preliminary experiments gave an approx. 48 kDa band in human brain (cerebellum, cerebral cortex and frontal cortex) lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 38.8 kDa band according to NP_071343.2. The 48 kDa band was successfully blocked by incubation with the immunizing peptide.
Control
ABHD4 Overexpression Lysate
Publications
Read Publication using NBP1-06009.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ABHD4 Antibody

  • ABH4
  • abhydrolase domain containing 4
  • abhydrolase domain-containing protein 4
  • Alpha/beta-hydrolase 4
  • EC 3.1.1.-
  • FLJ12816
  • Lyso-N-acylphosphatidylethanolamine lipase

Background

Lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE). Contributes to the biosynthesis ofN-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains fromN-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate forN-acyl ethanolamine biosynthesis. Hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acylchains. Shows no significant activity towards other lysophospholipids, including lysophosphatidylcholine,lysophosphatidylethanolamine and lysophosphatidylserine

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ABHD4 Antibody (NBP1-06009)(1)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ABHD4
Entrez
Uniprot