ABHD4 Antibody

Immunohistochemistry-Paraffin: ABHD4 Antibody [NBP1-06009] - (3.75ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Ca
• Applications: IHC, IHC-P, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ABHD4 Antibody Summary

• Immunogen: Peptide with sequence C-RPTNPSEIRAP corresponding to internal region according to NP_071343.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ABHD4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:4000
• Application Notes: WB: Preliminary experiments gave an approx. 48 kDa band in human brain (cerebellum, cerebral cortex and frontal cortex) lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 38.8 kDa band according to NP_071343.2. The 48 kDa band was successfully blocked by incubation with the immunizing peptide.
• Control:

  ABHD4 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for ABHD4 Antibody

• ABH4
• abhydrolase domain containing 4
• abhydrolase domain-containing protein 4
• Alpha/beta-hydrolase 4
• EC 3.1.1.-
• FLJ12816
• Lyso-N-acylphosphatidylethanolamine lipase

Background

Lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE). Contributes to the biosynthesis ofN-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains fromN-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate forN-acyl ethanolamine biosynthesis. Hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acylchains. Shows no significant activity towards other lysophospholipids, including lysophosphatidylcholine,lysophosphatidylethanolamine and lysophosphatidylserine

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ABHD4 Antibody (NBP1-06009)(1)

Publication using NBP1-06009

• Simon GM, Cravatt BF. Endocannabinoid biosynthesis proceeding through glycerophospho-N-acyl ethanolamine and a role for alpha/beta-hydrolase 4 in this pathway. J Biol Chem 2006-09-08 [PMID: 16818490]

Bioinformatics

• Gene Symbol: ABHD4
• Entrez:
  • 63874(Human)
  • 105501(Mouse)
• Uniprot:
  • Q8TB40(Human)