ABCD4 Antibody

Western Blot: ABCD4 Antibody [NBP1-20872] - (0.3ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected with chemiluminescence.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ABCD4 Antibody Summary

• Description: Novus Biologicals Goat ABCD4 Antibody (NBP1-20872) is a polyclonal antibody validated for use in WB and ELISA. Anti-ABCD4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-RDDIDNPDQRISQD corresponding to internal region according to NP_005041.1.
• Epitope: C terminus
• Specificity: Reported variants represent identical protein: NP_064719.1 NP_064731.1 NP_064720.1 NP_064730.1
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ABCD4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 70 kDa band observed in lysates of Jurkat and MOLT4 (calculated MW of 68.6 kDa band according to NP_005041.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ABCD4 Antibody

• ATP-binding cassette sub-family D member 4
• ATP-binding cassette, sub-family D (ALD), member 4
• EST352188
• P70R69 kDa peroxisomal ABC-transporter
• Peroxisomal membrane protein 1-like
• Peroxisomal membrane protein 69
• PMP69P79R
• PMP70-related protein
• PXMP1-L
• PXMP1LABC41

Background

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ALD subfamily, which is involved in peroxisomal import of fatty acids and/or fatty acyl-CoAs in the organelle. All known peroxisomal ABC transporters are half transporters which require a partner half transporter molecule to form a functional homodimeric or heterodimeric transporter. The function of this peroxisomal membrane protein is unknown. However, it is speculated that it may function as a heterodimer for another peroxisomal ABC transporter and, therefore, may modify the adrenoleukodystrophy phenotype. It may also play a role in the process of peroxisome biogenesis. Alternative splicing results in at least two different transcript variants, one which is protein-coding and one which is probably not protein-coding.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ABCD4 Antibody (NBP1-20872)(1)

Publication using NBP1-20872

• Asheuer M, Bieche I, Laurendeau I et al. Decreased expression of ABCD4 and BG1 genes early in the pathogenesis of X-linked adrenoleukodystrophy. Hum Mol Genet 2005-05-15 [PMID: 15800013]

Bioinformatics

• Gene Symbol: ABCD4
• Entrez:
  • 5826(Human)
  • 19300(Mouse)
  • 299196(Rat)
• Uniprot:
  • NP_005041.1(Human)