ABCB9 Antibody

Immunohistochemistry-Paraffin: ABCB9 Antibody [NB300-865] - (3.8ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining

• Reactivity: Hu
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ABCB9 Antibody Summary

• Immunogen: Peptide with sequence C-GHNEPVANGSHKA corresponding to C-Terminus according to NP_062570.1, NP_062571.1, NP_982269.1.
• Specificity: This antibody is expected to recognise both human isoforms of this protein (as represented by NP_062570.1, NP_062571.1and NP_982269.1). Variants (NP_062571.1; NP_982269.1) encode the same isoform.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ABCB9
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:64000
• Application Notes: WB: Preliminary experiments gave no signal but low background in human brain and liver lysates at up to 1 ug/ml. IHC-P: Human testis shows vesiculate cytoplasm staining of primary spermatocytes.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ABCB9 Antibody

• ABC transporter 9 protein
• ATP-binding cassette sub-family B member 9
• ATP-binding cassette transporter 9
• ATP-binding cassette, sub-family B (MDR/TAP), member 9
• EC 3.6.3
• EC 3.6.3.44
• EST122234
• hABCB9
• KIAA1520
• TAPL
• TAP-like protein

Background

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. The function of this half-transporter has not yet been determined; however, this protein may play a role in lysosomes. Alternative splicing of this gene results in distinct isoforms which are likely to have different substrate specifications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ABCB9 Antibody (NB300-865)(1)

Publication using NB300-865

• Zhang F, Zhang W, Liu L et al. Characterization of ABCB9, an ATP binding cassette protein associated with lysosomes. J Biol Chem 2000-07-28 [PMID: 10748049]

Bioinformatics

• Gene Symbol: ABCB9
• Entrez:
  • 23457(Human)
• Uniprot:
  • Q9NP78(Human)