AAK1 Antibody (4B10) - Azide and BSA Free

Western Blot: AAK1 Antibody (4B10) [H00022848-M02] - Analysis of AAK1 expression in transfected 293T cell line by AAK1 monoclonal antibody (M02), clone 4B10.Lane 1: AAK1 transfected lysate(52 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: AAK1 Antibody (4B10) [H00022848-M02] - Analysis of monoclonal antibody to AAK1 on HeLa cell . Antibody concentration 10 ug/ml.

Sandwich ELISA: AAK1 Antibody (4B10) [H00022848-M02] - Detection limit for recombinant GST tagged AAK1 is 0.1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 4B10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

AAK1 Antibody (4B10) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse AAK1 Antibody (4B10) - Azide and BSA Free (H00022848-M02) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: AAK1 (AAH02695, 315 a.a. ~ 415 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.LKKECPIPNVQNSPIPAKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASV
• Specificity: AAK1 - AP2 associated kinase 1 (4B10)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: AAK1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against transfected lysate and recombinant protein for Western Blot. Has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for AAK1 Antibody (4B10) - Azide and BSA Free

• AAK1
• Adaptor-associated kinase 1
• AP2 associated kinase 1
• AP2-associated protein kinase 1
• DKFZp686F03202
• DKFZp686K16132
• EC 2.7.11.1
• FLJ23712
• FLJ25931
• FLJ31060
• FLJ45252
• KIAA1048FLJ42882
• MGC138170
• MGC164568
• MGC164570

Background

Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AAK1
• Entrez:
  • 22848(Human)
• Uniprot:
  • AAH02695(Human)