Paxillin Antibody

Product Discontinued

Paxillin Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids N-terminus of Human Paxillin.
• Marker: Focal Adhesion Marker
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PXN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analyzes of Paxillin (L25)pAb in extracts from COS-7 cells.
  Immunohistochemistry (IHC) analyzes of Paxillin (L25) pAb in paraffin-embedded human breast carcinoma tissue.
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Paxillin Antibody

• FLJ16691
• Paxillin
• PXN

Background

Paxillin is a focal adhesion phosphoprotein that is localized to the cytoskeleton. Phosphorylation of paxillin has been shown to occur in response to PDGF treatment, v-Src transformation or cross-linking of integrins. FAK (focal adhesion kinase) and PYK2 have been shown to phosphorylate paxillin. FAK phosphorylates paxillin specifically on Tyr 118 in vitro. However, FAK phosphorylation does not seem to be required for the recruitment of paxillin to cell adhesion sites. Paxillin may play a role in signal transduction, regulation of cell morphology and the recruitment of structural and signaling molecules to focal adhesions. It has been shown that the amount of paxillin is reduced in mitotic cells by proteolytic downregulation and that paxillin is alternatively phosphorylated on serine rather than on tyrosine and serine during mitosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Paxillin Antibody (NBP1-19833)(2)

Publications using NBP1-19833

• Foxall E, Staszowska A, Hirvonen LM, et al. PAK4 Kinase Activity Plays a Crucial Role in the Podosome Ring of Myeloid Cells Cell Rep 2019-12-10 [PMID: 31825823] (Human)
• Longo G, Ioannidu CA, Scotto d'Abusco A et al. Improving Osteoblast Response In Vitro by a Nanostructured Thin Film with Titanium Carbide and Titanium Oxides Clustered around Graphitic Carbon. PLoS ONE. 2016-04-01 [PMID: 27031101] (ICC/IF, Human)

Review for Paxillin Antibody (NBP1-19833) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Elizabeth Foxall IF Human 03/28/2014

Summary

• Application: Immunofluorescence
• Sample Tested: fixed Human monocytic leukaemia (THP-1) cells
• Species: Human
• Lot: G22121

Bioinformatics

• Gene Symbol: PXN
• Entrez:
  • 5829(Human)
  • 19303(Mouse)
  • 360820(Rat)
• Uniprot:
  • P49023()