58K Golgi Protein Antibody

Western Blot: 58K Golgi Protein Antibody [NB100-1448] - Staining of liver lysates (A) Human with antibody at 0.1 ug/mL, Mouse (B) and Pig (C) with antibody at 0.03 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: 58K Golgi Protein Antibody [NB100-1448] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Flow Cytometry: 58K Golgi Protein Antibody [NB100-1448] - Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunocytochemistry/Immunofluorescence: 58K Golgi Protein Antibody [NB100-1448] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

• Reactivity: Hu, Mu, Po, Rt, Bv
• Applications: WB, ELISA, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

58K Golgi Protein Antibody Summary

• Immunogen: This 58K Golgi Protein Antibody was developed against a peptide with sequence CLREQGRGKDQPGRL corresponding to internal region according to NP_006648.1, NP_996848.1.
• Localization: Cytoplasmic and Golgi Apparatus
• Marker: Golgi Apparatus Marker
• Specificity: Variants (NP_006648.1; NP_996848.1) encode the same protein.
• Predicted Species: Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FTCD
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.1 - 0.3 ug/mL
• Application Notes: ICC/IF usage is validated and reported in scientific literature (PMID: 16534631).
• Control:

  Human Liver Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for 58K Golgi Protein Antibody

• formimidoyltransferase cyclodeaminase
• formimidoyltransferase-cyclodeaminase
• formiminotransferase cyclodeaminase
• Formiminotransferase-cyclodeaminase
• human formiminotransferase cyclodeaminase, EC 4.3.1.410formiminotransferase-cyclodeaminase
• LCHC1

Background

58K protein antibodies are excellent for use as markers for the Golgi complex. The 58K protein has been identified as being FTCD, a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Defects in FTCD are the cause of glutamate formiminotransferase deficiency; also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 58K Golgi Protein Antibody (NB100-1448)(2)

Publications using NB100-1448

• Hagiwara H, Tajika Y, Matsuzaki T et al. Localization of Golgi 58K protein (formiminotransferase cyclodeaminase) to the centrosome. Histochem Cell Biol. 2006-08-01 [PMID: 16534631] (ICC/IF)
  
  Details:
  Product cited: 58K Golgi protein (IMG-3369). IF/ICC: Staining of centrosomes in squamous carcinoma cell line derived from human tongue (HSC-4 cells), Fig 5.
• Bloom GS, Brashear TA. A novel 58-kDa protein associates with the Golgi apparatus and microtubules. J Biol Chem 1989-09-25 [PMID: 2777777]

Bioinformatics

• Gene Symbol: FTCD
• Entrez:
  • 10841(Human)
• Uniprot:
  • NP_006648(Human)