58K Golgi Protein Antibody

Images

 
Western Blot: 58K Golgi Protein Antibody [NB100-1448] - Analysis (0.03ug/ml) of Human Liver lysate (RIPA buffer, 35ug total protein per lane).
Immunohistochemistry-Paraffin: 58K Golgi Protein Antibody [NB100-1448] - (3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

58K Golgi Protein Antibody Summary

Immunogen
Peptide with sequence CLREQGRGKDQPGRL corresponding to internal region according to NP_006648.1, NP_996848.1.
Localization
Cytoplasmic and Golgi Apparatus
Marker
Golgi Apparatus Marker
Specificity
Variants (NP_006648.1; NP_996848.1) encode the same protein.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FTCD
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.03-0.1 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 3-5 ug/ml
  • Peptide ELISA detection limit 1:64000
Application Notes
WB: Approx. 60 kDa band observed in human liver lysates (calculated MW of 58.9 kDa band according to NP_006648.1and NP_996848.1).
IHC-P: Human liver shows distinct staining of membranes and cytoplasm in a selection of hepatocytes.
ICC/IF usage is reported in scientific literature (PMID: 16534631).
Positive Control
Liver Lysate (NB820-59232)
Publications
Read Publications using NB100-1448.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Porcine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for 58K Golgi Protein Antibody

  • formimidoyltransferase cyclodeaminase
  • formimidoyltransferase-cyclodeaminase
  • formiminotransferase cyclodeaminase
  • Formiminotransferase-cyclodeaminase
  • human formiminotransferase cyclodeaminase, EC 4.3.1.410formiminotransferase-cyclodeaminase
  • LCHC1

Background

58K protein antibodies are excellent for use as markers for the Golgi complex. The 58K protein has been identified as being FTCD, a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Defects in FTCD are the cause of glutamate formiminotransferase deficiency; also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for 58K Golgi Protein Antibody (NB100-1448)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FTCD
Entrez
Uniprot