Western Blot: 58K Golgi Protein Antibody [NB100-1448] - Analysis (0.03ug/ml) of Human Liver lysate (RIPA buffer, 35ug total protein per lane).
Immunohistochemistry-Paraffin: 58K Golgi Protein Antibody [NB100-1448] - (3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: 58K Golgi Protein Antibody [NB100-1448] - (0.01ug/ml) Human Liver lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
WB: Approx. 60 kDa band observed in human liver lysates (calculated MW of 58.9 kDa band according to NP_006648.1and NP_996848.1). IHC-P: Human liver shows distinct staining of membranes and cytoplasm in a selection of hepatocytes. ICC/IF usage is reported in scientific literature (PMID: 16534631).
Predicted cross-reactivity based on sequence identity: Mouse, Rat, Porcine.
Alternate Names for 58K Golgi Protein Antibody
human formiminotransferase cyclodeaminase, EC 22.214.171.1240formiminotransferase-cyclodeaminase
58K protein antibodies are excellent for use as markers for the Golgi complex. The 58K protein has been identified as being FTCD, a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Defects in FTCD are the cause of glutamate formiminotransferase deficiency; also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.