58K Golgi Protein Antibody (FTCD/357) [mFluor Violet 610 SE]

58K Golgi Protein Antibody (FTCD/357) [mFluor Violet 610 SE] [NBP3-14274MFV610] - Vial of mFluor Violet 610 conjugated antibody. mFluor Violet 610 is optimally excited at 421 nm by the Violet laser (405 nm) and has an emission maximum of 613 nm.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF
• Clone: FTCD/357
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: mFluor Violet 610 SE

58K Golgi Protein Antibody (FTCD/357) [mFluor Violet 610 SE] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: 58K Golgi Protein Protein purified from rat liver. (Uniprot: O95954)
• Localization: Cytoplasm, Golgi apparatus
• Specificity: The antibody recognizes an epitope located on the microtubule-binding peripheral Golgi membrane 58 kDa protein. It is also useful for studies on the effect of microtubule-perturbing agents on the Golgi apparatus. The protein encoded by this gene is a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Mutations in this gene are associated with glutamate formiminotransferase deficiency. Alternatively-spliced transcript variants have been found for this gene. Folate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool. Binds and promotes bundling of vimentin filaments originating from the Golgi.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FTCD
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NBP3-14274. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Reactivity Notes

Predicted to react in Mouse, Rat, Bovine, Canine, Hamster, Porcine, Monkey and Kangaroo Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for 58K Golgi Protein Antibody (FTCD/357) [mFluor Violet 610 SE]

• formimidoyltransferase cyclodeaminase
• formimidoyltransferase-cyclodeaminase
• formiminotransferase cyclodeaminase
• Formiminotransferase-cyclodeaminase
• human formiminotransferase cyclodeaminase, EC 4.3.1.410formiminotransferase-cyclodeaminase
• LCHC1

Background

58K protein antibodies are excellent for use as markers for the Golgi complex. The 58K protein has been identified as being FTCD, a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Defects in FTCD are the cause of glutamate formiminotransferase deficiency; also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FTCD