5-HT3B Antibody

Western Blot: 5-HT3B Antibody [NBP1-51969] - (1ug/ml) staining of Human Cerebral Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

5-HT3B Antibody Summary

• Description: Novus Biologicals Goat 5-HT3B Antibody (NBP1-51969) is a polyclonal antibody validated for use in WB and ELISA. Anti-5-HT3B Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-SNYLQTQDQTDQQE corresponding to internal region (near C-terminus) according to NP_006019.1.
• Epitope: C-SNYLQTQDQTDQQE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HTR3B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 51 kDa band observed in human brain (cerebral cortex, hippocampus and amygdala) lysates (calculated MW of 50.3 kDa band according to NP_006019.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for 5-HT3B Antibody

• 5HT3B
• 5-HT3B
• 5-HT3-B
• 5-HT3B5-hydroxytryptamine receptor 3B
• 5-hydroxytryptamine (serotonin) receptor 3B
• 5-hydroxytryptamine 3 receptor B subunit
• HTR3B
• Serotonin receptor 3B
• serotonin-gated ion channel subunit

Background

The product of the HT3B belongs to the ligand-gated ion channel receptor superfamily. This gene encodes subunit B of the type 3 receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor causes fast, depolarizing responses in neurons after activation. It appears that the heteromeric combination of A and B subunits is necessary to provide the full functional features of this receptor, since either subunit alone results in receptors with very low conductance and response amplitude. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 5-HT3B Antibody (NBP1-51969)(1)

Publication using NBP1-51969

• Krzywkowski K, Davies PA, Feinberg-Zadek PL et al. High-frequency HTR3B variant associated with major depression dramatically augments the signaling of the human 5-HT3AB receptor. Proc Natl Acad Sci U S A 2008-01-01 [PMID: 18184810]

Bioinformatics

• Gene Symbol: HTR3B
• Entrez:
  • 9177(Human)
• Uniprot:
  • NP_006019.1(Human)