4EBP1 Antibody (4B6G10)

Western Blot: 4EBP1 Antibody (4B6G10) [NBP1-47554] - Analysis using 4E-BP1 mouse mAb against truncated 4E-BP1 recombinant protein(1)and A431 cell lysate (2).

Immunohistochemistry-Paraffin: 4EBP1 Antibody (4B6G10) [NBP1-47554] - Analysis of human pancreas carcinoma (A), esophagus carcinoma tissue (B) and ovary tumor tissue (C), showing cytoplasmic and membrane localization using 4E-BP1 mouse mAb with DAB staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IHC-P
• Clone: 4B6G10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

4EBP1 Antibody (4B6G10) Summary

• Immunogen: Purified recombinant fragment of 4E-BP1 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: EIF4EBP1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
• Control:

  4EBP1 Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for 4EBP1 Antibody (4B6G10)

• 4EBP1
• 4E-BP1
• eIF4E-binding protein 1
• EIF4EBP1
• eukaryotic translation initiation factor 4E binding protein 1,4EBP1,4E-BP1BP-1
• eukaryotic translation initiation factor 4E-binding protein 1
• PHAS-I
• PHAS-IMGC4316
• Phosphorylated heat- and acid-stable protein regulated by insulin 1

Background

4E-BP1(eukaryotic translation Initiation Factor 4E Binding Protein 1),also called ELF4EBP1/BP-1/PHAS-I,which is located on chromosome 8p12, with 118-amino acid protein (about 13kDa). Binding of eIF4EBP1 to eIF4E is reversible and is dependent on the phosphorylation status of eIF4EBP1. Non phosphorylated eIF4EBP1 will bind strongly to eIF4E while(24kDa), the phosphorylated form will not. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating eIF4EBP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the eIF4EBP1-eIF4E interaction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF4EBP1
• Entrez:
  • 1978(Human)