17 beta-HSD14/HSD17B14 Antibody (736043)

17 beta -HSD14/HSD17B14 was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human 17 beta -HSD14/HSD17B14 Monoclonal Antibody (Catalog # MAB7515) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clone: 736043
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

17 beta-HSD14/HSD17B14 Antibody (736043) Summary

• Immunogen: E. coli-derived recombinant human 17 beta ‑HSD14/HSD17B14
  Ala2-Ser270
  Accession # Q9BPX1
• Specificity: Detects human 17 beta ‑HSD14/HSD17B14 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSD17B14
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for 17 beta-HSD14/HSD17B14 Antibody (736043)

• 17 betaHSD14
• 17 beta-HSD14
• dehydrogenase/reductase (SDR family) member 10
• Dehydrogenase/reductase SDR family member 10
• DHRS10
• DHRS1017-beta-hydroxysteroid dehydrogenase 14
• EC 1.1.1.-
• HSD17B14
• hydroxysteroid (17-beta) dehydrogenase 14,17-beta-hydroxysteroid dehydrogenase DHRS10
• retinal short-chain dehydrogenase/reductase 3
• Retinal short-chain dehydrogenase/reductase retSDR3
• retSDR3
• RETSDR3,17-beta-HSD 14
• SDR3
• SDR47C1
• short chain dehydrogenase/reductase family 47C, member 1

Background

HSD-17b14 (hydroxysteroid (17-beta) dehydrogenase type14), also called 17bHSD14 or DHRS10, is a cytoplasmic 17beta-hydroxysteroid dehydrogenase that is a member of the SDR (short-chain dehydrogenase/reductase) family. It is a 270 amino acid, approximately 30 kDa protein with mRNA highly expressed in brain, placenta, liver and kidney. It converts estradiol to estrone, thus inactivating it, and is thought to regulate activity of estrogens and possibly dihydroepiandrosterone (DHEA) in the placenta and central nervous system. Human HSD-17b14 shares 80.5% amino acid sequence identity with mouse and rat HSD-17b14.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSD17B14
• Uniprot:
  • Q9BPX1(Human)