14-3-3 tau/theta Antibody (1A1) - BSA Free

Western Blot: 14-3-3 tau/theta Antibody (1A1) [NBP1-21301] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 tau antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : MDA-MB-21 cell lysateLane 2. : HeLa cell lysateLane 3. : A431 cell lysateLane 4. : NIH3T3 cell lysateLane 5. : 293T cell lysateLane 6. : HepG2 cell lysate

Immunocytochemistry/Immunofluorescence: 14-3-3 tau/theta Antibody (1A1) [NBP1-21301] - Analysis in HeLa cells line, stained with DAPI (Blue) for nucleus staining and 1:100 with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Western Blot: 14-3-3 tau/theta Antibody (1A1) [NBP1-21301] - 14-3-3 tau/theta Antibody (1A1) Cell lysates of Jurkat (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human 14-3-3 theta (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Western Blot: 14-3-3 tau/theta Antibody (1A1) [NBP1-21301] - The recombinant proteins (50ng) were resolved by SDSPAGE, transferred to PDVF membrane and probed with antihuman 14-3-3 tau antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : Recombinant Human YWHAZLane 2. : Recombinant Human YWHABLane 3. : Recombinant Human YWHAELane 4. : Recombinant Human YWHAHLane 5. : Recombinant Human YWHAGLane 6. : Recombinant Human SFNLane 7. : Recombinant Human YWHAQ (14-3-3 tau/theta)

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 1A1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

14-3-3 tau/theta Antibody (1A1) - BSA Free Summary

• Description: Novus Biologicals Mouse 14-3-3 tau/theta Antibody (1A1) - BSA Free (NBP1-21301) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-14-3-3 tau/theta Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human 14-3-3 tau/theta (1-245aa) purified from E. coli (NP_006817).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: YWHAQ
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000-1:2000
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for 14-3-3 tau/theta Antibody (1A1) - BSA Free

• 14-3-3 protein tau
• 14-3-3 protein T-cell
• 14-3-3 protein theta
• 1433 theta
• 14-3-3 theta
• 14-3-3,1C5
• HS1 protein
• HS1
• Protein HS1
• protein tau
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, thetapolypeptide
• YWHAQ

Background

14-3-3 theta belongs to the 14-3-3 family of proteins that mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and it is 99% identical to the mouse and rat orthologs. 14-3-3 theta is upregulated in patients with amyotrophic lateral sclerosis (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 14-3-3 tau/theta Antibody (NBP1-21301)(2)

Publications using NBP1-21301

• Brandwein D Investigating 14-3-3 Protein Subcellular Localization, Colocalization with Subcellular Markers and Interaction with Rac1 Thesis (ICC/IF, WB, Human, Monkey)
• Abdrabou A, Brandwein D, Wang Z Differential Subcellular Distribution and Translocation of Seven 14-3-3 Isoforms in Response to EGF and During the Cell Cycle Int J Mol Sci 2020-01-02 [PMID: 31906564] (ICC/IF, Human)

Review for 14-3-3 tau/theta Antibody (NBP1-21301) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Yongkang Yang WB Human 03/20/2021

Summary

• Application: Western Blot
• Sample Tested: 293T and MCF-7 cells-whole cell lysate,MDA MB 231 cells
• Species: Human
• Lot: A1814601

Bioinformatics

• Gene Symbol: YWHAQ
• Entrez:
  • 10971(Human)
• Uniprot:
  • P27348()