14-3-3 epsilon Antibody

Product Discontinued

14-3-3 epsilon Antibody Summary

• Immunogen: Synthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3 epsilon.
• Specificity: This recognises the acetylated N-terminal of 14-3-3 epsilon. It may not react with recombinant proteins that are not N-acetylated.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: YWHAE
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: This antibody is useful for Immunohistochemistry-Paraffin, ELISA, Western Blot. In Western Blot it detects a band/s of approximately 30kDa in HEK293 cell lysates. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.

Reactivity Notes

This antibody is reactive against Sheep, Human, Rat, Mouse, Bovine, Chicken, Rabbit.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.09% Sodium Azide
• Purity: Unpurified

Alternate Names for 14-3-3 epsilon Antibody

• 1433 epsilon
• 14-3-3 epsilon
• 14-3-3 protein epsilon
• FLJ45465
• FLJ53559,14-3-3E
• KCIP-1
• MDCR
• MDS
• mitochondrial import stimulation factor L subunit
• protein kinase C inhibitor protein-1
• tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilonpolypeptide
• YWHAE

Background

Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation amoung many other functions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: YWHAE
• Entrez:
  • 7531(Human)