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![Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. HIF-1 alpha antibody (H1alpha67) [NB100-105]. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-900WB-img0019.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. HIF-1 alpha antibody (H1alpha67) [NB100-105]. Western blot image submitted by a verified customer review.")
![<b>Biological Strategies Validation. </b>Simple Western: HIF-1 alpha Antibody Pack [NB100-900WB] - Simple Western lane view shows a specific band for HIF-1 alpha in 0.5 mg/mL of hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. HIF-1 alpha Antibody [NB100-449].](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Simple-Western-NB100-900WB-img0018.jpg)
![Simple Western: HIF-1 alpha Antibody Pack [NB100-900WB] - Image shows a specific band for HIF-1 alpha in 0.2 mg/mL of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Simple-Western-NB100-900WB-img0022.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - Naive CD4 T cells from WT, VHL-deficient (Vhl KO), or HIF-1 alpha-deficient (HIF-1 alpha KO) mice were differentiated under IL-22-skewing conditions for a total of 60 h. Some cells remained at normoxia for the duration of the culture (N); others were at normoxia for 35 h and then hypoxia (1% O2) for 24 h (H). At 60 h, nuclear extracts were harvested, and HIF-1 alpha and Lamin B1 levels were analyzed by Western blot. Image from verified customer review. HIF-1 alpha Antibody (H1alpha67) [NB100-105]](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Knockout-Validated-NB100-900WB-img0020.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - Normoxic pre-treatment sensitizes glioma cells to radiation after graded chronic hypoxic (GCH) exposure. The results of anchorage-independent colony forming assays are shown for U87, U87-luc, GL261 glioma cells and 0308 GSCs after 5 Gy radiation exposure under varying O2 conditions. For easier comparisons among cell types, raw values are expressed as a % of the cell type's negative (non-irradiated) control and the means & SEMs are plotted. Also shown are Western blots of nuclear HIF-1alpha at the time of irradiation. Corresponding Western blots of lamin A/C are shown as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0111199) licensed under a CC-BY license. HIF-1 alpha Antibody [NB100-479]](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-900WB-img0015.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - Morphological alterations induced by DMOG are HIF-1alpha dependent. glEND.2 cells were stably transfected with shGFP, shHIF-1alpha or shHIF-2alpha. The clones were cultured under normoxic (N) or hypoxic (H, 1% O2) conditions, or treated with DMOG for 6 h. 30 ug protein of cellular protein were loaded for the detection of HIF-1alpha, and nuclear extracts (20 ug protein) were used for the detection of HIF-2alpha. Image collected and cropped by CiteAb from the following publication (//biosignaling.biomedcentral.com/articles/10.1186/1478-811X-11-80) licensed under a CC-BY license. HIF-1 alpha Antibody [NB100-449]](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Knockdown-Validated-NB100-900WB-img0017.jpg)
![Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - Analysis of HIF-1 alpha in overexpression and endogenous HIF-1 alpha & HIF-2 alpha using anti-HIF-1 alpha antibody NB100-134. The data showed that HIF-1 alpha antibody did not react to HIF-2 alpha overexpression. Wester blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-900WB-img0021.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - HIF-1 alpha induction on Caki-1 cell lysate using CoCl2. Western blot image submitted by a verified customer review. HIF-1 alpha Antibody [NB100-449]](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-900WB-img0016.jpg)
![Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - Detection of HIF-1 alpha (125-130 kDa) from human placental villous explant total protein. HIF-1 alpha Antibody (HA111) [NB100-296]](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-900WB-img0023.jpg)
![Western Blot: LC3 Antibody Pack [NB910-40435] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions. LC3B Antibody [NB100-2220]](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Knockout-Validated-NB910-40435-img0013.jpg "<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: LC3 Antibody Pack [NB910-40435] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions. LC3B Antibody [NB100-2220]")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Immunohistochemistry: LC3 Antibody Pack [NB910-40435] - Rapamycin increases autophagy in brains of PDAPP mice. Representative epifluorescent (c200x) image of hippocampal CA1 in control- and rapamycin-fed transgenic PDAPP mice stained with an anti-LC3 antibody. An increase in LC3-immunoreactive puncta was observed in CA1 projections of transgenic PDAPP mice following rapamycin administration. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0009979) licensed under a CC-BY license. LC3A Antibody [NB100-2331]](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Immunohistochemistry-NB910-40435-img0009.jpg)
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: LC3 Antibody Pack [NB910-40435] - LC3B was detected in immersion fixed Cloroquine treated HeLa cells (left) but was not detected in LC3B knockout HeLa cells (right) using rabbit anti-human LC3B polyclonal antibody (Catalog #NB100-2220) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Knockout-Validated-NB910-40435-img0014.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: LC3 Antibody Pack [NB910-40435] - High autophagosome concentration is consumed during early immortalized human mesenchymal stem cell differentiation. Immortalized human mesenchymal stem cells were differentiated under osteogenic conditions (see Materials and methods) and assayed for changes in LC3I and LC3II during a 72-hour window. Cells were differentiated under standard conditions (top) or with addition of 5 uM rapamycin (middle) or 5 nM bafilomycin (bottom) for the first 3 hours of differentiation to modulate autophagy. Immunoblots were performed for LC3 at the indicated time points to assess autophagosome degradation via relative changes in LC3II (lower band; 17 kDa). Studies were repeated three times with similar trends seen consistently. Image collected and cropped by CiteAb from the following publication (//stemcellres.com/content/5/6/140), licensed under a CC-BY license. LC3A Antibody [NB100-2331]](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Western-Blot-NB910-40435-img0011.jpg)
![Immunohistochemistry: LC3 Antibody Pack [NB910-40435] - Localization of LC3 by immunohistochemical method. The immunohistochemical staining shows that LC3, CRF, and HIF-1alpha have an overlapping localization in villous and extravillous trophoblast. d: decidua; v: villi; DeVe: decidual vessel. Original magnification 40x. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2013/689768/) licensed under a CC-BY license. LC3B Antibody [NB100-2220]](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Immunohistochemistry-NB910-40435-img0015.jpg)
![Immunohistochemistry: LC3 Antibody Pack [NB910-40435] - Brain, Cerebral Cortex, Cell Processes in Gray Matter 40x.](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Immunohistochemistry-NB910-40435-img0008.jpg)
![Immunohistochemistry: LC3 Antibody Pack [NB910-40435] - Analysis in PFA fixed NIH/3T3 cells using anti-LC3A antibody. Image from verified customer review. LC3A Antibody [NB100-2331]](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Immunohistochemistry-NB910-40435-img0012.jpg)
![Immunocytochemistry/ Immunofluorescence: LC3 Antibody Pack [NB910-40435] - The autophagy level was increased in degenerated mouse SGNs. (a-c) Western blot results revealed that the levels of the autophagy-related proteins LC3 & BECN1 were increased in the degenerated SGNs on the 5th, 15th & 30th day after ototoxic drug administration & were significantly different from those in the normal mice. *, P < 0.05. (d) Immunofluorescence staining of LC3 puncta (red) also demonstrated that the LC3 level in the degenerated SGNs (green) was significantly increased on the 30th day after drug administration. Con, normal mice without drug treatment; 30D, 30 days after drug administration. Images of immunofluorescence staining were taken from the middle turn of cochlea. Scale bar: 10 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30706760), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb910-40435_lc3-antibody-pack-31020241538249.jpg)
![Western Blot: LC3 Antibody Pack [NB910-40435] - The autophagy level was increased in degenerated mouse SGNs. (a-c) Western blot results revealed that the levels of the autophagy-related proteins LC3 & BECN1 were increased in the degenerated SGNs on the 5th, 15th & 30th day after ototoxic drug administration & were significantly different from those in the normal mice. *, P < 0.05. (d) Immunofluorescence staining of LC3 puncta (red) also demonstrated that the LC3 level in the degenerated SGNs (green) was significantly increased on the 30th day after drug administration. Con, normal mice without drug treatment; 30D, 30 days after drug administration. Images of immunofluorescence staining were taken from the middle turn of cochlea. Scale bar: 10 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30706760), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb910-40435_lc3-antibody-pack-310202415284514.jpg)
![Western Blot: LC3 Antibody Pack [NB910-40435] - CCI-779 significantly rescued the impaired autophagy-lysosomal pathway in degenerated SGNs of mice. (a) The levels of Ctsb, Ctsd, & Lamp1 & of the autophagic genes Becn1 & Lc3b were significantly higher, & Sqstm1 was lower in the experimental group than in the negative control group, which was determined by quantitative real-time PCR. (b & c) The LAMP1 & CTSD levels determined by western blotting were consistent with the quantitative real-time PCR results. (d & e) Compared with those in the negative control group, the LC3 & BECN1 levels in the experimental groups were significantly increased, as determined by western blot assays. (f & g) The western blot results revealed that the levels of the autophagic cargo receptor SQSTM1 & ubiquitinated proteins were decreased significantly in the experimental group compared with those in the negative control groups. *, the difference between the experimental group & the blank control group was significant (P <Â 0.05); #, the difference between the experimental group & the negative control group was significant (P <Â 0.05); CCI-779, experimental group; 30D, negative control group; Con, blank control group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30706760), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb910-40435_lc3-antibody-pack-310202416163727.jpg)
![Immunohistochemistry: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - Analysis of HIF-2 alpha in human endometrium using. Donkey anti-mouse Alexa Fluor 488 secondary antibody was used. Image from verified customer review. HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132]](http://images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-Pack-Immunohistochemistry-NB100-902HIF2-img0010.jpg "Immunohistochemistry: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - Analysis of HIF-2 alpha in human endometrium using. Donkey anti-mouse Alexa Fluor 488 secondary antibody was used. Image from verified customer review. HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132]")
![<b>Biological Strategies Validation. </b>Western Blot: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - WNT11 is induced by hypoxia or hypoxic mimetics in different cell types. Immunoblot analyses of HeLa cells under normal air or hypoxia for 24 hrs. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep21520) licensed under a CC-BY license. HIF-2 alpha/EPAS1 Antibody [NB100-122]](http://images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-Pack-Western-Blot-NB100-902HIF2-img0012.jpg)
![Simple Western: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - Lane view shows a specific band for HIF-2 alpha in 0.5 mg/ml of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132]](http://images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-Pack-Simple-Western-NB100-902HIF2-img0009.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - HIF-1alpha is the predominant transcriptional regulator of WNT11 expression during hypoxia. EMSCs isolated from the indicated mouse genotypes were infected with lentivirus expressing GFP or Cre recombinase. Non-infected cells and GFP infected cells served as controls. Immunoblot analyses of EMSCs derived from the indicated genotypes treated with 0.1 mM DMOG for 24 hrs. Attenuated WNT11 expression in Hif-1alpha KO EMSCs (lenti-Cre infected Hif-1af/f). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep21520) licensed under a CC-BY license. HIF-2 alpha/EPAS1 Antibody [NB100-122]](http://images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-Pack-Knockout-Validated-NB100-902HIF2-img0013.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - Formalin-fixed paraffin-embedded tissue sections of human placenta were probed for HIF-2 alpha/EPAS1 mRNA (ACD RNAScope Probe, catalog #410598; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using Rabbit Polyclonal (Novus Biologicals catalog # NB100-122) at 1:100 dilution with one-hour incubation at room temperature followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to trophoblastic cells. HIF-2 alpha/EPAS1 Antibody [NB100-122]](http://images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-Pack-Immunohistochemistry-NB100-902HIF2-img0014.jpg)
![Immunohistochemistry: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - Normal ganglia 1 (NG1), normal ganglia 2 (NG2) and IDH-mutant (IDH). Sporadic PGL (Spo. PGL). HIF2 alpha staining. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0127471) licensed under a CC-BY license. HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132]](http://images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-Pack-Immunohistochemistry-NB100-902HIF2-img0011.jpg)
![Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody Pack [NB100-926] - 53BP1 was detected in immersion fixed HeLa cells (left) but was not detected in 53BP1 knockout Hela cells (right) using Rabbit Anti-human 53BP1 polyclonal antibody (Catalog #NB100-304) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei.](http://images.novusbio.com/fullsize/53BP1-Antibody-Pack-Knockout-Validated-NB100-926-img0020.jpg "<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody Pack [NB100-926] - 53BP1 was detected in immersion fixed HeLa cells (left) but was not detected in 53BP1 knockout Hela cells (right) using Rabbit Anti-human 53BP1 polyclonal antibody (Catalog #NB100-304) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei.")
![<b>Genetic Strategies Validation. </b>Western Blot: 53BP1 Antibody Pack [NB100-926] - Western blot analysis of human 53BP1, blotted with 53BP1 antibody (Catalog #NB100-305). Sample: Whole cell lysate (20 ug/lane) from U2Os cells resolved on a 3 to 8% trisacetate gel. A band appears at approximately the theoretical molecular weight of 214 kDa.](http://images.novusbio.com/fullsize/53BP1-Antibody-Pack-Western-Blot-NB100-926-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: 53BP1 Antibody Pack [NB100-926] - Exosomes modulate the repair of DNA DSBs in irradiated recipient cells. Representative images of 53BP1 foci in BHY cells 6 hours after 2 Gy and transfer of BHY exosomes isolated 24 hours after irradiation with 0, 3, 6 or 9 Gy (53BP1 foci green, nuclei blue) using NB100-305. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0152213), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/53BP1-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB100-926-img0017.jpg)
![Immunohistochemistry: 53BP1 Antibody Pack [NB100-926] - Immunohistochemical analysis of human breast tumors stained with 53BP1 antibody (Catalog #NB100-304). Image from verified customer review.](http://images.novusbio.com/fullsize/53BP1-Antibody-Pack-NB100-926-img0021.jpg)
![Flow Cytometry: 53BP1 Antibody Pack [NB100-926] - An intracellular stain was performed on HeLa cells with 53BP1 Antibody (Catalog #NB100-305AF488) (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/53BP1-Antibody-Pack-Flow-Cytometry-NB100-926-img0023.jpg)
![Western Blot: 53BP1 Antibody Pack [NB100-926] - Detection of Human [deleted mouse] 53BP1 by Western Blot on Whole cell lysate from U2OS or 293T cells resolved on a 3 to 8% tris-acetate gel using 53BP1 Antibody (Catalog #NB100-304).](http://images.novusbio.com/fullsize/53BP1-Antibody-Pack-Western-Blot-NB100-926-img0009.jpg)
![Immunohistochemistry: 53BP1 Antibody Pack [NB100-926] - Immunohistochemical staining of placental villi with 53BP1 Antibody (Catalog #NB100-305) at 40X magnification.](http://images.novusbio.com/fullsize/53BP1-Antibody-Pack-Immunohistochemistry-NB100-926-img0012.jpg)
![Flow Cytometry: 53BP1 Antibody Pack [NB100-926] - Detection of 53BP1. 1 million Jurkat cells were fixed, permeabilized, and stained with 1.5 mcg/ml 53BP1 Antibody (Catalog #NB100-304) in a 150 mcl reaction. Isotype control (black), anti-53bp1 (red).](http://images.novusbio.com/fullsize/53BP1-Antibody-Pack-Flow-Cytometry-NB100-926-img0010.jpg)
![Flow Cytometry: 53BP1 Antibody Pack [NB100-926] - An intracellular stain was performed on RH-30 cells with 53BP1 Antibody (Catalog #NB100-304AF647) (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/53BP1-Antibody-Pack-Flow-Cytometry-NB100-926-img0022.jpg)
![Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. Image from verified customer review. HIF-1 alpha Antibody (H1alpha67) [NB100-105]](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-905-img0048.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. Image from verified customer review. HIF-1 alpha Antibody (H1alpha67) [NB100-105]")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody Pack [NB100-905] - HIF-1 alpha was detected in immersion fixed DFO treated Hela cells (left) but was not detected in HIF-1 knockout HeLa cells (right) using Mouse Anti-human HIF-1 alpha monoclonal antibody (Catalog #NB100-105) at 25 ug/mL for 3 hours at room temperature. Cells were stained using a NorthernLights (TM) 557-conjugated Donkey Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. HIF-1 alpha Antibody (H1alpha67) [NB100-105]](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Knockout-Validated-NB100-905-img0049.jpg)
![Chromatin Immunoprecipitation: HIF-1 alpha Antibody Pack [NB100-905] - ChIP-qPCR assay of HIF-1 binding to hypoxia response element (HRE) of the PKM2 gene in human MDA-MB-231 breast cancer cells treated with vehicle or DMOG (HIF inducer) for 8 hours. Negative control: immunoprecipitation was performed with IgG. Image courtesy of Gregg Semenza, Johns Hopkins University School of Medicine. HIF-1 alpha Antibody [NB100-479]](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Chromatin-Immunoprecipitation-NB100-905-img0041.jpg)
![Simple Western: HIF-1 alpha Antibody Pack [NB100-905] - Simple Western lane view shows a specific band for HIF-1 alpha in 0.5 mg/ml of hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. HIF-1 alpha Antibody [NB100-449]](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Simple-Western-NB100-905-img0042.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-905] - HIF-1alpha expression in PDAC and adjacent normal pancreatic tissue. PDAC with weak (intensity 1) nuclear and cytoplasmic HIF-1alpha staining (left). PDAC with strong (intensity 3) nuclear and moderate (intensity 2) cytoplasmic HIF-1alpha staining (right). Image collected and cropped by CiteAb from the following publication (//wjso.biomedcentral.com/articles/10.1186/s12957-018-1432-4), licensed under a CC-BY license. HIF-1 alpha Antibody (H1alpha67) [NB100-105]](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Immunohistochemistry-NB100-905-img0051.jpg)
![Flow Cytometry: HIF-1 alpha Antibody Pack [NB100-905] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-105. Staining of HIF-1 alpha in multiple myeloma cells: H929 cells (0.5x10^6) were stained with Alexa Fluor 488 (R) conjugated HIF-1 alpha antibody (NB100-105AF488). Image courtesy of Dr. Barbara Muz at Washington University in St. Louis School of Medicine. HIF-1 alpha Antibody (H1alpha67) [NB100-105]](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Flow-Cytometry-NB100-905-img0050.jpg)
![<b>Biological Strategies Validation, Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - Effects of in vitro hypoxia on the expression of mouse HIF-1 alpha in HIF-1 alpha+/- and HIF-1 alpha+/+ astrocytes. Confluent astrocyte monolayers of both cell types were exposed to a 6 h in vitro hypoxia. HIF-1 alpha mRNA expression was determined by RT-PCR as described in Materials and Methods. Each bar represents the mean +/- SD of relative density/volumes of the bands on film negatives from at least three experiments. Asterisks and number sign indicate significant difference (p < 0.01; one-way ANOVA, followed by multiple comparisons among means). Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-4-12), licensed under a CC-BY license. HIF-1 alpha Antibody [NB100-654]](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-905-img0053.jpg)
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody Pack [NB100-905] - Detection of HIF-1 alpha (red dye 568) in a cultured raw mouse macrophage cell line, using NB100-131. 100X magnification. Photos courtesy of Susan Alexander and Hattie Gresham, PhD. HIF-1 alpha Antibody (ESEE122) [NB100-131]](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB100-905-img0037.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - Normoxic pre-treatment sensitizes glioma cells to radiation after graded chronic hypoxic (GCH) exposure. The results of anchorage-independent colony forming assays are shown for U87, U87-luc, GL261 glioma cells and 0308 GSCs after 5 Gy radiation exposure under varying O2 conditions. For easier comparisons among cell types, raw values are expressed as a % of the cell type's negative (non-irradiated) control and the means & SEMs are plotted. Also shown are Western blots of nuclear HIF-1alpha at the time of irradiation. Corresponding Western blots of lamin A/C are shown as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0111199) licensed under a CC-BY license. HIF-1 alpha Antibody [NB100-479].](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-905-img0040.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - HIF-1 alpha induction on Caki-1 cell lysate using CoCl2. Image from verified customer review. HIF-1 alpha Antibody [NB100-449]](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-905-img0044.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - Effects of CoCl2 treatment on HIF-1 alpha expression in mouse astrocytes. Western blots using nuclear proteins show that both hypoxia (H) and CoCl2 (Co) up-regulated HIF-1 alpha protein in HIF-1 alpha+/+ and HIF-1 alpha+/- cells. There was no HIF-1 alpha protein detected in control cells. Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-4-12), licensed under a CC-BY license. HIF-1 alpha Antibody [NB100-654]](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-905-img0052.jpg)
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody Pack [NB100-905] - Detection of HIF-1 alpha (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with Staph Aureus, using NB100-131. 100X magnification. Blue dye is DAPI nuclea](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB100-905-img0035.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-905] - Staining in normal mouse kidney showing (A) staining with antibody and (B) blocking peptide used at 10-fold excess concentration. Images provided by Dr Yves Heremans. Please see the product review of NBC1-18422 for additional details. HIF-1 alpha Antibody [NB100-479]](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Immunohistochemistry-NB100-905-img0038.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-905] - Hif1alpha expression in preimplantation embryos. Co-staining of OCT4 and Hif1alpha in inner cell mass of blastocyst (top) and negative control images without primary antibodies (bottom). Scale bar: 30um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0154309) licensed under a CC-BY license. HIF-1 alpha Antibody [NB100-479]](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Immunohistochemistry-NB100-905-img0039.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-905] - Immunohistochemical analysis in non-GIST STS representing negative, and score 1-3 of Carbonic Anhydrase IX/CA9, GLUT-1, HIF-1 alpha, and HIF-2 alpha/EPAS1. non-GIST STS: non-gastrointestinal stromal tumor soft-tissue sarcomas, Carbonic Anhydrase IX/CA9: carbonic anhydrase IX, GLUT-1: glucose transporter-1, and HIF-1/2alpha: hypoxia induced factor 1/2alpha. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/sarcoma/2012/541650/), licensed under a CC-BY license. HIF-1 alpha Antibody (ESEE122) [NB100-131]](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Immunohistochemistry-NB100-905-img0045.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-905] - Analysis of HIF-1 alpha in paraffin-embedded mouse kidney tissue section using anti-HIF-1 alpha antibody. Image from verified customer review. HIF-1 alpha Antibody (ESEE122) [NB100-131]](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Immunohistochemistry-NB100-905-img0047.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - Morphological alterations induced by DMOG are HIF-1alpha dependent. glEND.2 cells were stably transfected with shGFP, shHIF-1alpha or shHIF-2alpha. The clones were cultured under normoxic (N) or hypoxic (H, 1% O2) conditions, or treated with DMOG for 6 h. 30 ug protein of cellular protein were loaded for the detection of HIF-1alpha, and nuclear extracts (20 ug protein) were used for the detection of HIF-2alpha. Image collected and cropped by CiteAb from the following publication (//biosignaling.biomedcentral.com/articles/10.1186/1478-811X-11-80) licensed under a CC-BY license. HIF-1 alpha Antibody [NB100-449]](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Knockdown-Validated-NB100-905-img0043.jpg)
![Western Blot: Autophagy Antibody Pack [NB910-94159] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Knockout-Validated-NB910-94159-img0019.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Autophagy Antibody Pack [NB910-94159] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: Autophagy Antibody Pack [NB910-94159] - LC3B was detected in immersion fixed Cloroquine treated HeLa cells (left) but was not detected in LC3B knockout HeLa cells (right) using rabbit anti-human LC3B polyclonal antibody (Catalog #NB100-2220) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Knockout-Validated-NB910-94159-img0020.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Autophagy Antibody Pack [NB910-94159] - ERK phosphorylation in Atg5-/-cells depends on nutrient availability. Serum-deprived Atg5-/- MEFs display decreased ERK and p90RSK phosphorylation. Immunoblots for the indicated proteins in total lysates from 2 h serum-deprived WT and Atg5-/- MEFs. The bars represent mean+/-s.e.m. *P<0.05, ***P<0.001 compared with Con; Student's t-test, n=3. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms3799), licensed under a CC-BY license. ATG5 was detected using NB110-53818.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Knockout-Validated-NB910-94159-img0022.jpg)
![Immunohistochemistry: Autophagy Antibody Pack [NB910-94159] - Staining of ATG5 using NB110-53818 in human hepatocytes (liver) at 2.5ug/ml. 40X magnification.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94159-img0015.jpg)
![Simple Western: Autophagy Antibody Pack [NB910-94159] - Lane view shows a specific band for Beclin using NB110-87318 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Simple-Western-NB910-94159-img0029.jpg)
![Flow Cytometry: Autophagy Antibody Pack [NB910-94159] - An intracellular stain was performed on Hela Cells with NB110-56893 to detect ATG9 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG APC-conjugated Secondary Antibody (R&D Systems, F0111).image 5 of 5previousnextclose](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Flow-Cytometry-NB910-94159-img0027.jpg)
![Immunohistochemistry: Autophagy Antibody Pack [NB910-94159] - Analysis of a FFPE tissue section of human breast cancer using Beclin 1 antibody (NB110-87318) at 1:300 dilution, followed by a HRP labeled secondary antibody and DAB. Nuclei of the cells were counterstained with hematoxylin. The signal is strongest in a subset of stromal cells, likely the cancer associated fibroblasts.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94159-img0030.jpg)
![Immunohistochemistry: Autophagy Antibody Pack [NB910-94159] - SCI leads to lysosomal dysfunction. Representative high magnification images ( x 60) of LC3 (NB100-2220, green) and CTSD (red) staining in VH of gray matter from sham and day 1 SCI animals. Decrease in number and intensity of CTSD puncta (lysosomes) and increase in LC3 puncta (autophagosomes) is apparent after SCI. Scale bar is 10 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2014527) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94159-img0021.jpg)
![Western Blot: Autophagy Antibody Pack [NB910-94159] - Total protein from human HeLa and mouse Neuro2A cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Beclin-1 (NB110-87318) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94159-img0028.jpg)
![Western Blot: Autophagy Antibody Pack [NB910-94159] - Analysis of ATG5 in mouse wildtype ES cell lysate (Lane 1) and mouse ATG5 KO ES cell lysate (- cnt'l) (Lane 2). Atg5-/- ES cells from Dr. Noboru Mizushima [PMID: 11266458] Photo courtesy of Dr. Beth Levine, UT SW Med Center.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94159-img0016.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Autophagy Antibody Pack [NB910-94159] - Detection of ATG9A protein in HEK293 lysates. Lane 1: siRNA ATG9A knockdown Lane 2: wildtype ATG9A.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94159-img0017.jpg)
![Western Blot: Autophagy Antibody Pack [NB910-94159] - Detection of ATG16L1. Lane 1: NIH/3T3 Lane 2: HeLa .](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94159-img0018.jpg)
![Western Blot: Autophagy Antibody Pack [NB910-94159] - CD133 promoted glucose uptake. E. Detection of autophagic genes in Huh-7con and Huh-7sh133 cells by Western blotting. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0056878) licensed under a CC-BY license. ATG9 detected using NB110-56893](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94159-img0024.jpg)
![Western Blot: Autophagy Antibody Pack [NB910-94159] - Detection of ATG9A protein using NB110-56893 in HEK293 lysates. Lane 1: siRNA ATG9A knockdown Lane 2: wildtype ATG9A.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94159-img0025.jpg)
![Western Blot: LC3 Antibody Pack [NB910-40752] - LC3/MAP1LC3A Antibody Pack [NB910-40752] - Detection of LC3B in treated U87-MG (human glioblastoma astrocytoma) lysates using NB600-1384.](http://images.novusbio.com/fullsize/LC3-MAP1LC3A-Antibody-Pack-Western-Blot-NB910-40752-img0026.jpg "<b>Biological Strategies Validation. </b>Western Blot: LC3 Antibody Pack [NB910-40752] - LC3/MAP1LC3A Antibody Pack [NB910-40752] - Detection of LC3B in treated U87-MG (human glioblastoma astrocytoma) lysates using NB600-1384.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: LC3 Antibody Pack [NB910-40752] - LC3/MAP1LC3A Antibody Pack [NB910-40752] - Immunofluorescent staining of treated U373-MG cells using NB600-1384. The nuclei were stained with DAPI.](http://images.novusbio.com/fullsize/LC3-MAP1LC3A-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB910-40752-img0017.jpg)
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: LC3 Antibody Pack [NB910-40752] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions. LC3B Antibody [NB100-2220]](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Knockout-Validated-NB910-40752-img0027.jpg)
![Immunohistochemistry: LC3 Antibody Pack [NB910-40752] - Autophagosomes accumulate in neurons of the VH gray matter at day 1 after SCI. Representative images of IHC staining for LC3 (green) and neuronal marker NeuN (red) in VH of gray matter from sham and SCI animals. Stronger co-localization between NeuN and LC3 is apparent at day 1 after SCI. Scale bar is 20 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2014527) licensed under a CC-BY license. LC3B Antibody [NB100-2220]](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Immunohistochemistry-NB910-40752-img0029.jpg)
![Immunohistochemistry: LC3 Antibody Pack [NB910-40752] - Analysis in mouse renal tissue. Image from verifed customer review. LC3A Antibody [NB100-2331]](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Immunohistochemistry-NB910-40752-img0030.jpg)
![Western Blot: LC3/MAP1LC3A Antibody Pack [NB910-40752] - Detection of autophagic LC3 in mouse ES cell lysate using NB 100-2331. The atg5-/- lane (ES cells, cultured to form embryonic bodies, that are deficient in conversion of LC3-1 to LC3-11) demonstrates the specificity of NB 100-2331, as there is no detection of LC3-11. Photo courtesy of Dr. Beth Levine, UT Southwestern Medical Center.](http://images.novusbio.com/fullsize/LC3-MAP1LC3A-Antibody-Pack-Western-Blot-NB910-40752-img0025.jpg)
![Immunohistochemistry: LC3/MAP1LC3A Antibody Pack [NB910-40752] - Staining of treated U373-MG (human glioblastoma) cells using NB600-1384.](http://images.novusbio.com/fullsize/LC3-MAP1LC3A-Antibody-Pack-Immunohistochemistry-NB910-40752-img0021.jpg)
![Western Blot: APE Antibody Pack [NB100-909] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-APE-1 (NB100-116) in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/APE-Antibody-Pack-NB100-909-img0013.jpg "Western Blot: APE Antibody Pack [NB100-909] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-APE-1 (NB100-116) in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: APE Antibody Pack [NB100-909] - HeLa cells stained with anti-APE Antibody (NB100-116, Green) detected with DyLight Fluor 488 conjugated anti-rabbit IgG secondary antibody. Nuclei are counterstained with Hoechst 33258 (Blue).](http://images.novusbio.com/fullsize/APE-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB100-909-img0014.jpg)
![Immunohistochemistry: APE Antibody Pack [NB100-909] - Immunohistochemical staining of APE1 using NB100-116. A. Nuclear staining in the luminal epithelium of normal breast ducts and lobules. B. Low, and C. high nuclear APE1 expression in invasive breast cancer. (magnification x400). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0099528) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/APE-Antibody-Pack-Immunohistochemistry-NB100-909-img0015.jpg)
![Western Blot: APE Antibody Pack [NB100-909] - Analysis of APE1 using NB100-101 in cell lysates: 1. HeLa, 2. Ntera2, 3. A431, 4. HepG2, 5. MCF7, 6. NIH 3T3, 7. PC12, and 8. Cos 7.](http://images.novusbio.com/fullsize/APE-Antibody-Pack-NB100-909-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: APE Antibody Pack [NB100-909] - Detection of APE1 using NB100-101 in HeLa cells. Image from verified customer review.](http://images.novusbio.com/fullsize/APE-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB100-909-img0012.jpg)
![Immunohistochemistry: APE1 Antibody Pack [NB100-909] - Staining of APE-ref-1 in prostate cancer, using NB100-101.](http://images.novusbio.com/fullsize/APE1-Antibody-Pack-Immunohistochemistry-NB100-909-img0005.jpg)
![Simple Western: APE Antibody Pack [NB100-909] - Lane view shows a specific band for APE1 using NB100-101 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/APE-Antibody-Pack-Simple-Western-NB100-909-img0010.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: APE Antibody Pack [NB100-909] - Repression of p21 by APE1 in p53-null cells and effect of ectopic p53 in this repression. Representative Western analysis of p53, APE1 (using NB100-116), p21 and alpha-Tubulin levels in the same HCT116p53null cells. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0068467) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/APE-Antibody-Pack-Knockdown-Validated-NB100-909-img0018.jpg)
![Western Blot: ARNT/HIF-1 beta Antibody Pack [NB100-982] - Analysis of ARNT/HIF-1 beta using ARNT/HIF-1 beta antibody [NB100-110] from ARNT/HIF-1 beta antibody pack [NB100-982] in MCF7 whole cell lysate. Theoretical molecular weight of ARNT/HIF-1 beta is 86.6 kDa, observed molecular weight ~90 kDa.](http://images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-Pack-Western-Blot-NB100-982-img0010.jpg "Western Blot: ARNT/HIF-1 beta Antibody Pack [NB100-982] - Analysis of ARNT/HIF-1 beta using ARNT/HIF-1 beta antibody [NB100-110] from ARNT/HIF-1 beta antibody pack [NB100-982] in MCF7 whole cell lysate. Theoretical molecular weight of ARNT/HIF-1 beta is 86.6 kDa, observed molecular weight ~90 kDa.")
![Immunohistochemistry: ARNT/HIF-1 beta Antibody Pack [NB100-982] - Staining of skin, epidermis using ARNT/HIF-1 beta antibody [NB100-110] from the ARNT/HIF-1 beta antibody pack [NB100-982].](http://images.novusbio.com/fullsize/HIF-1-beta-Antibody-Pack-Immunohistochemistry-NB100-982-img0005.jpg)
![Chromatin Immunoprecipitation: ARNT/HIF-1 beta Antibody Pack [NB100-982] - Allele specific HIF-binding at the rs12814794 associated enhancer. B) Allele-specific qPCR for rs12814794 from DNA fragments isolated in HIF-ChIP experiments (HIF-1A and ARNT/HIF-1 beta) or input DNA from primary renal tubular cells (PTC, n = 4 individuals). DNA from individuals homozygous for the AA or GG genotype were used as positive controls. C) Quantification of the two different alleles (A or G) at rs12814794 in the ChIP-seq reads from HIF-1A and ARNT/HIF-1 beta antibody [NB100-110] immunoprecipitations (PTC #1 Fig 3). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pgen.1006872) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-Pack-Chromatin-Immunoprecipitation-NB100-982-img0009.jpg)
![Western Blot: ARNT/HIF-1 beta Antibody Pack [NB100-982] - Expression of CD44 mRNA in genetically engineered MDA-MB-231 cells. (A) Immunoblot analysis of HIF-1a or HIF-2a expression in whole cell extracts from MDA-MB-231 cells stably expressing EV, HIF-1a-shRNA or HIF-2a-shRNA under normoxia or in response to 4 h treatment with 200 uM CoCl2. ARNT/HIF-1 beta expression was used as a loading control. Theoretical molecular weight of ARNT/HIF-1 beta is 86.6 kDa. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0044078) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-Pack-Western-Blot-NB100-982-img0006.jpg)
![Western Blot: ARNT/HIF-1 beta Antibody Pack [NB100-982] - HIF was not the only factor stabilizing activated EGFR in VHL-deficient ccRCC cells. A. Western blot analysis of 786-VHL and 786-mock cells stably expressing shRNA constructs. For HIF2A [NB100-480] and ARNT/HIF-1 beta [NB100-124] analysis, nuclear extracts were generated and analyzed. Anti-HA blot detected HA-VHL. Means and SDs of three separate experiments were shown. Theoretical molecular weight of ARNT/HIF-1 beta is 86.6 kDa, observed molecular weight ~90 kDa. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0023936) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-Pack-Western-Blot-NB100-982-img0007.jpg)
![Immunohistochemistry: ARNT/HIF-1 beta Antibody Pack [NB100-982] - Staining of human glioblastoma multi-forme utilizing the ARNT/HIF-1 beta antibody[NB100-124] from the ARNT/HIF-1 beta antibody pack [NB100-982].](http://images.novusbio.com/fullsize/HIF-1-beta-Antibody-Pack-Immunohistochemistry-NB100-982-img0004.jpg)
![Dual RNAscope ISH-IHC: Survivin Antibody Pack [NB100-911] - Formalin-fixed paraffin-embedded tissue sections of human esophagus squamous cell carcinoma were probed for Survivin mRNA (ACD RNAScope Probe, [465361]; Fast Red chromogen, ACD [322360]). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal [NB500-201] at 1.5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to tumor cells.](http://images.novusbio.com/fullsize/Survivin-Antibody-Pack-RNAscope-compatible-NB100-911-img0006.jpg "Dual RNAscope ISH-IHC: Survivin Antibody Pack [NB100-911] - Formalin-fixed paraffin-embedded tissue sections of human esophagus squamous cell carcinoma were probed for Survivin mRNA (ACD RNAScope Probe, [465361]; Fast Red chromogen, ACD [322360]). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal [NB500-201] at 1.5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to tumor cells.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: Survivin Antibody Pack [NB100-911] - Anti-tumor effects and functional evidence of sCA-survivin-siRNA in HCT116 and HT29 solid tumor models. Immunostaining of survivin in the tumor tissues on day 19 using [NB500-201]. Scale bar, 50 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0116022) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Survivin-Antibody-Pack-Knockdown-Validated-NB100-911-img0008.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Survivin Antibody Pack [NB100-911] - Suppression of survivin expression by transfection of siRNA. RNA interference was performed in SW1353 and Hs 819.T, either for GFP as control or for survivin. A: A pronounced decrease of survivin protein levels was measured by immunoblotting in SW1353 and Hs819.T. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-11-120), licensed under a CC-BY license. Survivin Antibody (60.11) [NB500-238]](http://images.novusbio.com/fullsize/Survivin-Antibody-Pack-Knockdown-Validated-NB100-911-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Survivin Antibody Pack [NB100-911] - Analysis using the HRP conjugate of [NB500-201]. Staining of Telophase with accumulation of survivin in the midbodies of two daughter cells. Survivin detection using [NB500-201].](http://images.novusbio.com/fullsize/Survivin-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB100-911-img0007.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: Survivin Antibody Pack [NB100-911] - Low-power image of human high-grade chondrosarcoma displays strong cellular expression of survivin protein (A). High-power magnification reveals the predominantly cytoplasmic staining, although strong nuclear signals are detectable (B). Other specimen of a grade III chondrosarcoma stained with monoclonal antibody, shows a similar pattern of staining (C and D). For A and B the polyclonal rabbit anti-survivin antibody [AF886] was used. For C and D the monoclonal mouse Survivin Antibody (60.11) [NB500-238] was used. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-11-120), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Survivin-Antibody-Pack-Immunohistochemistry-NB100-911-img0005.jpg)
![Immunohistochemistry-Paraffin: Survivin Antibody Pack [NB100-911] - Immunohistochemical staining of formalin-fixed paraffin-embedded human lung cancer tissue using Survivin Antibody (32.1) [NB500-237].](http://images.novusbio.com/fullsize/Survivin-Antibody-Pack-Immunohistochemistry-Paraffin-NB100-911-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - The stem cell marker (Nanog) staining of iPS-BP cells. Nuclei were stained with DAPI (blue). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0044024) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Pluripotent-Stem-Cell-Transcription-Factor-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NBP1-42823-img0014.jpg "Immunocytochemistry/Immunofluorescence: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - The stem cell marker (Nanog) staining of iPS-BP cells. Nuclei were stained with DAPI (blue). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0044024) licensed under a CC-BY license.")
![Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - YM155 significantly reduced tumorsphere formation and inhibited EGFR autophosphorylation and G9a expression. YM155 is reported to suppress cancer stemness; therefore, we used this compound to investigate the cellular mechanism of tumorsphere formation. To compare the cytotoxic capacity of YM155 against tumorsphere formation and parental cell lines, YM155 was applied to HCC827 and A549 cells in the stemness cultured or integrated medium. (F) Moreover, YM155 inhibited EGFR autophosphorylation in A549-derived tumorspheres and reduced Oct4 expression. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0182149) licensed under a CC-BY license. OCT4 Antibody [NB100-2379]](http://images.novusbio.com/fullsize/Pluripotent-Stem-Cell-Transcription-Factor-Antibody-Pack-NBP1-42823-img0016.jpg)
![Western Blot: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - Detection of SOX2 in mouse brain lysate using NB110-37235 (0.5ug/ml).](http://images.novusbio.com/fullsize/Pluripotent-Stem-Cell-Transcription-Factor-Antibody-Pack-Western-Blot-NBP1-42823-img0013.jpg)
![Immunohistochemistry: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - Nanog-inducible mouse model. Representative hematoxylin and eosin (H&E) staining and immunohistochemistry (IHC) for NANOG, Ki67 and LORICRIN of TPA-treated CTR and TG mice (bars correspond to 50 um). Statistical significance was determined by the two-tailed Student's t test: (*) p < 0.05; (**) p < 0.01. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep10205), licensed under a CC-BY license. Nanog Antibody [NB100-58842]](http://images.novusbio.com/fullsize/Pluripotent-Stem-Cell-Transcription-Factor-Antibody-Pack-Immunohistochemistry-NBP1-42823-img0015.jpg)
![Western Blot: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - Whole cell lysate (5, 15, and 50 ug) from F9 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nanog antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds](http://images.novusbio.com/fullsize/Pluripotent-Stem-Cell-Transcription-Factor-Antibody-Pack-Western-Blot-NBP1-42823-img0011.jpg)
![Immunohistochemistry: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - SOX2 staining (NB110-37235) of human uterus, endometrial glands.](http://images.novusbio.com/fullsize/Pluripotent-Stem-Cell-Transcription-Factor-Antibody-Pack-Immunohistochemistry-NBP1-42823-img0017.jpg)
![Western Blot: Autophagy Antibody Pack [NB910-94877] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Knockout-Validated-NB910-94877-img0027.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Autophagy Antibody Pack [NB910-94877] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: Autophagy Antibody Pack [NB910-94877] - LC3B was detected in immersion fixed Cloroquine treated HeLa cells (left) but was not detected in LC3B knockout HeLa cells (right) using rabbit anti-human LC3B polyclonal antibody (Catalog #NB100-2220) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Knockout-Validated-NB910-94877-img0028.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Autophagy Antibody Pack [NB910-94877] - ERK phosphorylation in Atg5-/-cells depends on nutrient availability. Serum-deprived Atg5-/- MEFs display decreased ERK and p90RSK phosphorylation. Immunoblots for the indicated proteins in total lysates from 2 h serum-deprived WT and Atg5-/- MEFs. The bars represent mean+/-s.e.m. *P<0.05, ***P<0.001 compared with Con; Student's t-test, n=3. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms3799), licensed under a CC-BY license. ATG antibody (NB110-53818) used.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Knockout-Validated-NB910-94877-img0031.jpg)
![Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - Staining of ATG5 using NB110-53818 in human hepatocytes (liver) at 2.5ug/ml. 40X magnification.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94877-img0025.jpg)
![Simple Western: Autophagy Antibody Pack [NB910-94877] - Lane view shows a specific band for Beclin using NB110-87318 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Simple-Western-NB910-94877-img0037.jpg)
![Flow Cytometry: Autophagy Antibody Pack [NB910-94877] - An intracellular stain was performed on Hela Cells with NB110-56893 to detect ATG9 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG APC-conjugated Secondary Antibody (R&D Systems, F0111).image 5 of 5previousnextclose](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Flow-Cytometry-NB910-94877-img0035.jpg)
![Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - Analysis of a FFPE tissue section of human breast cancer using Beclin 1 antibody (NB110-87318) at 1:300 dilution, followed by a HRP labeled secondary antibody and DAB. Nuclei of the cells were counterstained with hematoxylin. The signal is strongest in a subset of stromal cells, likely the cancer associated fibroblasts.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94877-img0038.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - SCI leads to lysosomal dysfunction. Representative high magnification images ( x 60) of LC3 (NB100-2220, green) and CTSD (red) staining in VH of gray matter from sham and day 1 SCI animals. Decrease in number and intensity of CTSD puncta (lysosomes) and increase in LC3 puncta (autophagosomes) is apparent after SCI. Scale bar is 10 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2014527) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94877-img0029.jpg)
![Western Blot: Autophagy Antibody Pack [NB910-94877] - Total protein from human HeLa and mouse Neuro2A cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Beclin-1 (NB110-87318) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94877-img0036.jpg)
![Western Blot: Autophagy Antibody Pack [NB910-94877] - Detection of ATG16L1 using NB110-60928 in HeLa whole cell extracts.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94877-img0040.jpg)
![Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human colon cancer using ATG16L1 antibody (NB110-60928) at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of ATG16L1 was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94877-img0041.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Autophagy Antibody Pack [NB910-94877] - Detection of ATG9A protein using NB110-56893 in HEK293 lysates. Lane 1: siRNA ATG9A knockdown Lane 2: wildtype ATG9A.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94877-img0017.jpg)
![Western Blot: Autophagy Antibody Pack [NB910-94877] - CD133 promoted glucose uptake. E. Detection of autophagic genes in Huh-7con and Huh-7sh133 cells by Western blotting. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0056878) licensed under a CC-BY license. ATG9 detected using NB110-56893](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94877-img0032.jpg)
![Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - Detection of ATG9A (NB110-56893) in mouse intestine.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94877-img0018.jpg)