Pluripotent Stem Cell Transcription Factor Antibody Pack

Images

 
Western Blot: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - Detection of SOX2 in mouse brain lysate using NB110-37235 (0.5ug/ml).
Immunohistochemistry: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - Staining of uterus, endometrial glands using NB110-37235.
Western Blot: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - analysis of Sox2 and Oct 4 in human breast cancer cell lines (MDA-MB-231 and MDA-MB-435) using anti-Sox2 antibody (NB110-37235) and ...read more
Western Blot: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - Mouse Nanog
Western Blot: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - Whole cell lysate (5, 15, and 50 ug) from F9 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nanog ...read more

Product Details

Summary
Reactivity Hu, Mu, Po, Bv, Ch, Pm, ShSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit

Order Details

Pluripotent Stem Cell Transcription Factor Antibody Pack Summary

Immunogen
NB100-58842: A synthetic peptide made to the mouse Nanog protein (within residues 1-50). [Swiss-Prot Q80Z64]. NB100-2379-A synthetic peptide made to an internal portion
Specificity
NB100-58842 is specific for mouse Nanog protein. NB100-2379-OCT4 - Embryonic Stem Cell Marker NB110-37235 - SOX2 - Embryonic Stem Cell Marker
Kit Type
Kit
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified

Applications/Dilutions

Application Notes
This antibody is useful for: NB100-37235 Western blot analysis where a band at ~40 kDa is be seen, representing the SOX2 protein. Has also been used in immunohistochemical and immunocytochemical applications. NB100-58842- Western blot analysis where a band at ~40 kDa is be seen, representing the SOX2 protein. Has also been used in immunohistochemical and immunocytochemical applications. NB100-2379-This antibody is useful for Western blot, where a band is seen at ~40 kDa. It is also useful for immunocytochemistry/immunofluorescence and flow cytometry.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-42823 in the following application:

Publications
Read Publication using
NBP1-42823 in the following applications:

  • WB
    1 publication

Reactivity Notes

NB100-58842-Mouse and Human. Reactivity with human Nanog is unlikely owing to divergence of sequence between this ortholog and mouse Nanog at the site to which the epitope maps. NB100-2379-Reacts with human, monkey and mouse. 93% sequence identity with cow and pig proteins NB110-37235 - Human, sheep and mouse. 92% sequence identity with chicken, 86% sequence identity with Xenopus, and 71% sequence identity with Zebrafish proteins.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Kit Components

Components
  1. Pluripotent Stem Cell Transcription Factor Antibody Pack (OCT4 Antibody)
  2. Pluripotent Stem Cell Transcription Factor Antibody Pack (SOX2 Antibody)
  3. NB100-58842 (Nanog Antibody)

Notes

Kit contains full sized units of the following: NB100-2379, NB110-37235, and NB100-58842.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

Publications for Pluripotent Stem Cell Transcription Factor Antibody Pack (NBP1-42823)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Review for Pluripotent Stem Cell Transcription Factor Antibody Pack (NBP1-42823) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-42823:
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WB
(1)
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Human
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Images Ratings Applications Species Date Details
Western Blot Pluripotent Stem Cell Transcription Factor NBP1-42823
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reviewed by:
Anonymous
WB Human 09/02/2015
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Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell whole cell lysate
SpeciesHuman

Blocking

Blocking DetailsBlocking with 5% non-fat milk for 30 min

Primary Anitbody

Dilution Ratio1:1000, overnight

Secondary Antibody

Secondary DescriptionDonkey anti-rabbit IgG, HRP
Secondary Manufacturer Cat#GE Healthcare, NA934V
Secondary Concentration1:2000

Details

Detection NotesRegular Western blot Protocol

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Pluripotent Stem Cell Transcription Factor Antibody Pack (NBP1-42823) (0)

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Recent Reviews

5
5
1
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0
1
0

Anonymous
09/02/2015
Application: WB
Species: Human