IL-10 Antibodies

417-ML), Recombinant Human IL-10 (Catalog # 217-IL), Recombinant Rat IL-10 (Catalog # 522-RLB), and Recombinant Human IL-26/AK155 Monomer (Catalog # 1375-IL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-417-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox"> 417-ML), Recombinant Human IL-10 (Catalog # 217-IL), Recombinant Rat IL-10 (Catalog # 522-RLB), and Recombinant Human IL-26/AK155 Monomer (Catalog # 1375-IL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-417-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." alt="Western blot shows 25 ng of Recombinant Mouse IL-10 (Catalog # 417-ML), Recombinant Human IL-10 (Catalog # 217-IL), Recombinant Rat IL-10 (Catalog # 522-RLB), and Recombinant Human IL-26/AK155 Monomer (Catalog # 1375-IL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-417-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_thumb" />

417-ML) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-10 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-417-NA). The ND₅₀ is typically 0.01-0.05 µg/mL." class="big_lightbox"> 417-ML) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-10 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-417-NA). The ND₅₀ is typically 0.01-0.05 µg/mL." class="big_thumb" />

417-ML) was serially diluted and captured by Rat Anti-Mouse IL‑10 Monoclonal Antibody (Catalog # MAB417) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-417-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 417-ML) was serially diluted and captured by Rat Anti-Mouse IL‑10 Monoclonal Antibody (Catalog # MAB417) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-417-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

417-ML) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑10 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑10 Polyclonal Antibody (Catalog # AB-417-NA). The ND₅₀ is typically 2-4 µg/mL." class="big_lightbox"> 417-ML) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑10 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑10 Polyclonal Antibody (Catalog # AB-417-NA). The ND₅₀ is typically 2-4 µg/mL." alt="Recombinant Mouse IL‑10 (Catalog # 417-ML) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑10 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑10 Polyclonal Antibody (Catalog # AB-417-NA). The ND₅₀ is typically 2-4 µg/mL." class="big_thumb" />

693-PI) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-10 (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine IL-10 Mono-clonal Antibody (Catalog # MAB6931). The ND₅₀ is typically 0.05-0.15 µg/mL." class="big_lightbox"> 693-PI) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-10 (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine IL-10 Mono-clonal Antibody (Catalog # MAB6931). The ND₅₀ is typically 0.05-0.15 µg/mL." alt="Recombinant Porcine IL-10 (Catalog # 693-PI) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-10 (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine IL-10 Mono-clonal Antibody (Catalog # MAB6931). The ND₅₀ is typically 0.05-0.15 µg/mL." class="big_thumb" />

VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="IL-10 was detected in immersion fixed paraffin-embedded sections of human tonil using Rat Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB92101) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IL-10 was detected in immersion fixed porcine peripheral blood mononuclear cells treated with calcium ionomycin and PMA using Mouse Anti-Porcine IL-10 Monoclonal Antibody (Catalog # MAB6932) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

HAF008). A specific band was detected for IL-10 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF008). A specific band was detected for IL-10 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB91842) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for IL-10 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in enteroendocrine cells of duodenum. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in enteroendocrine cells of duodenum. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

735-CL) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL‑10 (2 ng/mL) is neutralized (green line) by increasing concentrations of Canine IL‑10 Monoclonal Antibody (Catalog # MAB7352). The ND₅₀ is typically 0.1-0.25 µg/mL." class="big_lightbox"> 735-CL) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL‑10 (2 ng/mL) is neutralized (green line) by increasing concentrations of Canine IL‑10 Monoclonal Antibody (Catalog # MAB7352). The ND₅₀ is typically 0.1-0.25 µg/mL." alt="Recombinant Canine IL‑10 (Catalog # 735-CL) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL‑10 (2 ng/mL) is neutralized (green line) by increasing concentrations of Canine IL‑10 Monoclonal Antibody (Catalog # MAB7352). The ND₅₀ is typically 0.1-0.25 µg/mL." class="big_thumb" />

F0112) and Mouse Anti-Human CD4 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB3791N). Quadrant markers were set based on control antibody staining (Catalog # AB-105-C). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol. View our protocol for Staining Intracellular Molecules." class="big_lightbox"> F0112) and Mouse Anti-Human CD4 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB3791N). Quadrant markers were set based on control antibody staining (Catalog # AB-105-C). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol. View our protocol for Staining Intracellular Molecules." alt="Human peripheral blood mononuclear cells (PBMCs) either (A) stimulated to induce Th2 cells or (B) unstimulated were stained with Rabbit Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB9210) followed by Fluorescein-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0112) and Mouse Anti-Human CD4 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB3791N). Quadrant markers were set based on control antibody staining (Catalog # AB-105-C). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol. View our protocol for Staining Intracellular Molecules." class="big_thumb" />

217-IL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-10 (5 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB9210). The ND₅₀ is typically 75-450 ng/mL." class="big_lightbox"> 217-IL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-10 (5 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB9210). The ND₅₀ is typically 75-450 ng/mL." class="big_thumb" />