DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the membrane. Protocol available in
COMET™ Panel Builder.​" class="big_lightbox">
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the membrane. Protocol available in
COMET™ Panel Builder.​" alt="CEACAM-8 was detected in immersion fixed paraffin-embedded sections of human colon cancer using Mouse Anti-Human CEACAM-8, Monoclonal Antibody (Catalog #MAB4246) at 1ug/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module)and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog #
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the membrane. Protocol available in
COMET™ Panel Builder.​" class="big_thumb" />
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the membrane. Protocol available in
COMET™ Panel Builder.​" class="big_lightbox">
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the membrane. Protocol available in
COMET™ Panel Builder.​" class="big_thumb" />
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the membrane. Protocol available in
COMET™ Panel Builder." class="big_lightbox">
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the membrane. Protocol available in
COMET™ Panel Builder." class="big_thumb" />
HAF018). A specific band was detected for CEACAM-8/CD66b at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 1." class="big_lightbox">
HAF018). A specific band was detected for CEACAM-8/CD66b at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 1." class="big_thumb" />
MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #
F0101B)." class="big_lightbox">
MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #
F0101B)." class="big_thumb" />
VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog #
VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for
IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">
VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog #
VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for
IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />
HAF018). Specific bands were detected for CEACAM-1/CD66a at approximately 100-150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human liver tissue and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CEACAM-1/CD66a Monoclonal Antibody (Catalog # MAB22441) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for CEACAM-1/CD66a at approximately 100-150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
MAB0041, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins. " alt="HepG2 cells were stained with Mouse Anti-Human CEACAM‑1/CD66a Monoclonal Antibody (Catalog # MAB2244, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />
HAF016). Specific bands were detected for CEACAM-1/CD66a between approximately 110 and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse liver tissue and mouse small intestine tissue. PVDF Membrane was probed with 0.1 µg/mL of Mouse CEACAM-1/CD66a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6480) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for CEACAM-1/CD66a between approximately 110 and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.​" alt="CEACAM-8 was detected in immersion fixed paraffin-embedded sections of human colon cancer using Mouse Anti-Human CEACAM-8, Monoclonal Antibody (Catalog #MAB4246) at 1ug/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module)and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.​" class="big_thumb" />
HAF019). A specific band was detected for CEACAM-1/CD66a at approximately 100-150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CEACAM-1/CD66a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2244) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CEACAM-1/CD66a at approximately 100-150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B)." alt="Human whole blood granulocytes were stained with Mouse Anti-Human CEACAM-6/CD66c Monoclonal Antibody (Catalog # MAB3934, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B)." class="big_thumb" />
F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003)." alt="HEK293 human embryonic kidney cell line transfected with either (A) human CEACAM-5/CD66e or (B) human CEACAM-8/CD66b and eGFP was stained with Mouse Anti-Human CEACAM-5/CD66e Monoclonal Antibody (Catalog # MAB41281) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003)." class="big_thumb" />
![Immunohistochemistry-Paraffin: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Human spleen tissue section. Granulocytes stained positive for CD66b. IHC-P was performed using a Leica bond autostainer. Standard IHC protocol, ER2 retrieval, primary antibody was used at 1:500 in BSA/TBS. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CEACAM8-CD66b-Antibody-G10F5-Immunohistochemistry-Paraffin-NB100-77808-img0014.jpg "Immunohistochemistry-Paraffin: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Human spleen tissue section. Granulocytes stained positive for CD66b. IHC-P was performed using a Leica bond autostainer. Standard IHC protocol, ER2 retrieval, primary antibody was used at 1:500 in BSA/TBS. IHC-P image submitted by a verified customer review.")
![Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Human peripheral blood was stained with Mouse IgM isotype control (left panel), Mouse anti-Human CEACAM-8/CD66b clone G10F5 (right panel), followed by APC-conjugated Goat anti-Mouse IgM secondary antibody.](http://images.novusbio.com/fullsize/CEACAM8-CD66b-Antibody-G10F5-Flow-Cytometry-NB100-77808-img0012.jpg)
![Immunohistochemistry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Staining of CD66b on FFPE section of human liver. Secondary Antibody is A488 goat anti-mouse IgM. CD66b is shown in green and DAPI in blue. Cells exhibit a morphology that correspond to neutrophils with segmented nuclei. The CD66b Ab is highly specific with virtually no background signal. IHC image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CEACAM8-CD66b-Antibody-G10F5-Immunohistochemistry-NB100-77808-img0013.jpg)
![Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Human peripheral blood granulocytes stained with purified G10F5, followed by anti-mouse Igs FITC.](http://images.novusbio.com/fullsize/CEACAM8-CD66b-Antibody-G10F5-Flow-Cytometry-NB100-77808-img0001.jpg)
![Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the Alexa Fluor (R) 647 conjugate of NB100-77808. Staining of CD66b in human whole blood using anti-CD66b antibody. The primary antibody was used at a dilution of 1:100 and incubated for 25 minutes at 4C. Flow cytometry image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CEACAM8-CD66b-Antibody-G10F5-Flow-Cytometry-NB100-77808-img0007.jpg)
![Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the Alexa Fluor(R) 700 conjugate of NB100-77808. Staining of CD66b in human whole blood using anti-CD66b antibody conjugated with Alexa Fluor 700. The primary antibody was used at a dilution of 1:100 and incubated for 25 minutes at 4C. Flow cytometry image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CEACAM8-CD66b-Antibody-G10F5-Flow-Cytometry-NB100-77808-img0008.jpg)
![Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the DyLight 405 conjugate of NB100-77808. Staining of CD66b in human PBMCs using anti-CD66b antibody. Flow cytometry image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CEACAM8-CD66b-Antibody-G10F5-Flow-Cytometry-NB100-77808-img0009.jpg)
![Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the PE conjugate of NB100-77808. Staining of CD66b in human whole blood using anti-CD66b antibody conjugated with PE. The primary antibody was used at a dilution of 1:100 and incubated for 25 minutes at 4C. Flow cytometry image submitted by a verifed customer review.](http://images.novusbio.com/fullsize/CEACAM8-CD66b-Antibody-G10F5-Flow-Cytometry-NB100-77808-img0011.jpg)
![Flow Cytometry: CEACAM8/CD66b Antibody (G10F5) [NB100-77808] - Analysis using the FITC conjugate of NB100-77808. Staining of Human peripheral whole blood granulocytes G10F5 FITC.](http://images.novusbio.com/fullsize/CEACAM8-CD66b-Antibody-G10F5-Flow-Cytometry-NB100-77808-img0010.jpg)
![Immunohistochemistry: CEACAM-6/CD66c Antibody (1H7-4B) [NBP2-54626] - Analysis in human lung: Using Anti-CEACAM6 [1H7-4B] (10 ug/ml) developed with DAB-staining procedure. Dark line indicated shows CEACAM6 expression.](http://images.novusbio.com/fullsize/CEACAM-6-CD66c-Antibody-1H7-4B-Immunohistochemistry-NBP2-54626-img0003.jpg "Immunohistochemistry: CEACAM-6/CD66c Antibody (1H7-4B) [NBP2-54626] - Analysis in human lung: Using Anti-CEACAM6 [1H7-4B] (10 ug/ml) developed with DAB-staining procedure. Dark line indicated shows CEACAM6 expression.")
![Flow Cytometry: CEACAM-6/CD66c Antibody (1H7-4B) [NBP2-54626] - Binding of 10 ug/ml primary positive control mAb (upper panel), Anti-CEACAM6 [1H7-4B] (lower panel), negative isotype matched control mAb (thin line) on different CHO-human CEACAM transfected cells (the CEACAM family member expressed is stated above).](http://images.novusbio.com/fullsize/CEACAM-6-CD66c-Antibody-1H7-4B-Flow-Cytometry-NBP2-54626-img0002.jpg)
![ELISA: CEACAM-6/CD66c Antibody (1H7-4B) [NBP2-54626] - Solid phase was coated with 3 ug/ml Anti-CEA binding human CEACAM1-CEACAM8. After washing, blocking and coating human CEACAM1 antigen, detecting antibody Anti-CEACAM6 [1H7-4B] (10 ug/ml) followed by HRP-coupled goat anti-mouse Ig was added. TMB was used for visualizing the binding measured by Tecan-ELISA reader at 450 nm.](http://images.novusbio.com/fullsize/CEACAM-6-CD66c-Antibody-1H7-4B-ELISA-NBP2-54626-img0001.jpg)
![Immunoprecipitation: CEACAM-6/CD66c Antibody (1H7-4B) [NBP2-54626] - Human granulocyte lysate: Utilizing 10 ug/ml Anti-CEACAM6 [1H7-4B]. Detection of the sample using 5 ug/ml pAb Anti-CEA followed by HRP-coupled secondary pAb. Visualized by Chemiluminescence detection.](http://images.novusbio.com/fullsize/CEACAM-6-CD66c-Antibody-1H7-4B-Immunoprecipitation-NBP2-54626-img0004.jpg)
![Western Blot: CEACAM-6/CD66c Antibody (1H7-4B) [NBP2-54626] - Detection using 10 μg/ml NBP2-54626 followed by HRP-coupled secondary antibody. Visualized by Chemiluminescence detection.](http://images.novusbio.com/fullsize/antibody/nbp2-54626_mouse-ceacam6-cd66c-mab-1h7-4b-bsa-free-western-blot-382023125340..jpg)
CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to stromal cells. View our protocol for 
CTS002) and counterstained with hematoxylin (blue). View our protocol for 
2244-CM) was serially diluted and captured by Mouse Anti-Human CEACAM‑1/CD66a Monoclonal Antibody (Catalog # MAB22441) coated on a Clear Polystyrene Microplate (Catalog # ![Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CEA31) [NBP2-33012] - Human Colon Carcinoma stained with CEACAM5/CD66e Antibody (CEA31).](http://images.novusbio.com/fullsize/CEACAM5-CD66e-Antibody-CEA31-Immunohistochemistry-Paraffin-NBP2-33012-img0005.jpg "Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CEA31) [NBP2-33012] - Human Colon Carcinoma stained with CEACAM5/CD66e Antibody (CEA31).")
![Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CEA31) [NBP2-33012] - Human Colon Carcinoma stained with CEACAM5/CD66e Antibody (CEA31).](http://images.novusbio.com/fullsize/CEACAM5-CD66e-Antibody-CEA31-Immunohistochemistry-Paraffin-NBP2-33012-img0003.jpg)
![Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CEA31) [NBP2-33012] - Human Colon Carcinoma stained with CEACAM5/CD66e Antibody (CEA31).](http://images.novusbio.com/fullsize/CEACAM5-CD66e-Antibody-CEA31-Immunohistochemistry-Paraffin-NBP2-33012-img0004.jpg)
![Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CEA31) [NBP2-33012] - Analysis of CEACAM5/CD66e antibody (CEA31) on Human Colon Carcinoma. Incubation: 1 ug/mL for 30 minutes at room temperature. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-33012_mouse-monoclonal-ceacam5-cd66e-antibody-cea31-17720249362620.jpg)
![Western Blot: CEACAM-5/CD66e Antibody (5C8C4) [NBP2-54625] - Analysis of human granulocyte lysate: Detection using 10 ug/ml Anti-CEACAM5 [5C8C4] followed by HRP coupled secondary pAb. Visualized by Chemiluminescence detection.](http://images.novusbio.com/fullsize/CEACAM-5-CD66e-Antibody-5C8C4-Western-Blot-NBP2-54625-img0008.jpg "Western Blot: CEACAM-5/CD66e Antibody (5C8C4) [NBP2-54625] - Analysis of human granulocyte lysate: Detection using 10 ug/ml Anti-CEACAM5 [5C8C4] followed by HRP coupled secondary pAb. Visualized by Chemiluminescence detection.")
![Flow Cytometry: CEACAM-5/CD66e Antibody (5C8C4) [NBP2-54625] - Binding of 10 ug/ml primary positive control mAb (upper panel), Anti-CEACAM5 [5C8C4] (lower panel), negative isotype matched control mAb (thin line) on different CHO-human CEACAM transfected cells (the CEACAM family member expressed is stated above).](http://images.novusbio.com/fullsize/CEACAM-5-CD66e-Antibody-5C8C4-Flow-Cytometry-NBP2-54625-img0003.jpg)
![ELISA: CEACAM-5/CD66e Antibody (5C8C4) [NBP2-54625] - Solid phase was coated with 3 ug/ml Anti-CEA (Dako) binding human CEACAM1-CEACAM8. After washing, blocking and coating human CEACAM1 antigen, detecting antibody Anti-CEACAM5/8 [5C8C4] (10 ug/ml) followed by HRP-coupled goat anti-mouse Ig was added. TMB was used for visualizing the binding measured by Tecan-ELISA reader at 450 nm.](http://images.novusbio.com/fullsize/CEACAM-5-CD66e-Antibody-5C8C4-ELISA-NBP2-54625-img0002.jpg)
![Immunoprecipitation: CEACAM-5/CD66e Antibody (5C8C4) [NBP2-54625] - Human granulocyte lysate: Utilizing 10 ug/ml Anti-CEACAM5/8 [5C8C4]. Detection of the sample using 5 ug/ml pAb Anti-CEA followed by HRP coupled secondary pAb. Visualized by Chemiluminescence detection.](http://images.novusbio.com/fullsize/CEACAM-5-CD66e-Antibody-5C8C4-Immunoprecipitation-NBP2-54625-img0006.jpg)
F0126) and Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # 
NL010) and counterstained with DAPI (blue). Specific staining was localized to endothelial cells in bile canaliculi. View our protocol for 
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # 
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # 
HAF018). A specific band was detected for CEACAM-8/CD66b at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using 
MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # 
VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # 
CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in glandular epithelial cells. View our protocol for 
2244-CM) was serially diluted and captured by Mouse Anti-Human CEACAM‑1/CD66a Monoclonal Antibody (Catalog # 
F0101B). Quadrant markers were set based on control antibody staining (Catalog # 
VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes. View our protocol for 
