| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB172-SP | |||
| Carrier Free | FAB172V-100UG | |||
| Carrier Free | FAB172U-100UG | |||
| Carrier Free | FAB172T-100UG | |||
| Carrier Free | FAB172S-100UG | |||
| Carrier Free | FAB172R-100UG | |||
| Carrier Free | FAB172N-100UG | |||
| Carrier Free | FAB172G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-74396SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-24862 | |||
| BSA Free | NB100-56437SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 350 | FAB171U-100UG | |||
| Alexa Fluor 405 | FAB171V-100UG | |||
| Alexa Fluor 488 | FAB171G-100UG | |||
| Alexa Fluor 532 | FAB171X-100UG | |||
| Alexa Fluor 594 | FAB171T-100UG | |||
| Alexa Fluor 647 | FAB171R-100UG | |||
| Alexa Fluor 700 | FAB171N-100UG | |||
| Alexa Fluor 750 | FAB171S-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB171-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56437SS | |||
| BSA Free | NB100-56437 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77067SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76480-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77560SS | |||
![Immunocytochemistry/Immunofluorescence: CXCR4 Antibody [NB100-74396] - Optimization of immunofluorescence staining of CXCR4 protein. MDA, PC3, KG1 cells were used for the optimization experiments. Cells were seeded onto cell-tak coated 48 mm coverslips in a 48-well plate. Cells were fixed with 2% formaldehyde for 20 min, washed with PBS. After fixation, cells were blocked with 2.0% BSA in PBS for 1 h at room temperature. All the cells were incubated with a primary antibody for anti-rabbit CXCR4 for 1 h. After washing with PBS, cells were put in respective secondary antibodies-anti-rabbit dylight 405 for 1 h. PSMA= Magenta, EpCAM= Yellow, sLex= Green, CXCR4= Red, and Merge shows all the colors. MDA=PSMA+, EpCAM+, sLex+, CXCR4+. PC3 = PSMA-, EpCAM+, sLex-, CXCR4+. KG1 = PSMA-, EpCAM-, sLex+, CXCR4-. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0085143) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Immunocytochemistry-Immunofluorescence-NB100-74396-img0008.jpg "Immunocytochemistry/Immunofluorescence: CXCR4 Antibody [NB100-74396] - Optimization of immunofluorescence staining of CXCR4 protein. MDA, PC3, KG1 cells were used for the optimization experiments. Cells were seeded onto cell-tak coated 48 mm coverslips in a 48-well plate. Cells were fixed with 2% formaldehyde for 20 min, washed with PBS. After fixation, cells were blocked with 2.0% BSA in PBS for 1 h at room temperature. All the cells were incubated with a primary antibody for anti-rabbit CXCR4 for 1 h. After washing with PBS, cells were put in respective secondary antibodies-anti-rabbit dylight 405 for 1 h. PSMA= Magenta, EpCAM= Yellow, sLex= Green, CXCR4= Red, and Merge shows all the colors. MDA=PSMA+, EpCAM+, sLex+, CXCR4+. PC3 = PSMA-, EpCAM+, sLex-, CXCR4+. KG1 = PSMA-, EpCAM-, sLex+, CXCR4-. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0085143) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: CXCR4 Antibody [NB100-74396] - The A2AR and baicalin attenuated CXCR4 expression in the hypoxia-induced PAH mouse model. CXCR4 protein expression levels in lung tissue were examined by western blot. GAPDH served as an internal control (c, d; n = 3). Image collected and cropped by CiteAb from the following publication (//www.ncbi.nlm.nih.gov/pmc/articles/PMC5543745/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NB100-74396-img0012.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry: CXCR4 Antibody [NB100-74396] - Pep R54 in combination with nivolumab reduced the expression of CXCR4-CXCL12 and PD-L1 in PES43 tumors. Representative IHC pictures (magnification 400x) for CXCR4, CXCR7 (red staining), CXCL12, PD-1 and PD-L1 expression (brown staining) in PES43 collected tumors from mice treated with Pep R54, nivolumab or combined treatment. Image collected and cropped by CiteAb from the following publication (//jeccr.biomedcentral.com/articles/10.1186/s13046-019-1420-8) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Immunohistochemistry-NB100-74396-img0010.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry: CXCR4 Antibody [NB100-74396] - Pep R in combination with anti-PD-1 reduced the expression of CXCR4-CXCL12 and PD-L1 in MC38 tumors. Representative IHC pictures for CXCR4, CXCR7, CXCL12, PD-1 and PD-L1 expression (brown staining) in MC38 collected tumors (magnification 400x), from mice treated with Pep R, anti-murine PD-1 or combined treatment showing the reduction of CXCR4, CXCL12 and PD-L1 expression in mice treated with Pep R alone and in combination with anti-PD-1Image collected and cropped by CiteAb from the following publication (//jeccr.biomedcentral.com/articles/10.1186/s13046-019-1420-8) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Immunohistochemistry-NB100-74396-img0011.jpg)
![Western Blot: CXCR4 Antibody [NB100-74396] - Analysis of CXCR4 in HeLa whole cell extract.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NB100-74396-img0002.jpg)
![Western Blot: CXCR4 Antibody [NB100-74396] - Analysis of CXCR4 protein in human small intestine tissue lysate using 1:500 dilution of rabbit polyclonal CXCR4 antibody (Lot A2). The signal was developed using ECL method and the antibody generated a specific band of CXCR4 at ~40 kDa position.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NB100-74396-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: CXCR4 Antibody [NB100-74396] - CXCR4 antibody was tested in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/CXCR4-Antibody-Immunocytochemistry-Immunofluorescence-NB100-74396-img0003.jpg)
![Immunohistochemistry: CXCR4 Antibody [NB100-74396] - Immunostaining of CXCR4 in human cervical carcinoma tissue sections.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Immunohistochemistry-NB100-74396-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody [NB100-74396] - The A2AR & baicalin attenuated CXCR4 expression in the hypoxia-induced PAH mouse model. CXCR4 & MYH11 expression levels in mouse PASMCs were analyzed by immunofluorescence staining (a; n = 3). CXCR4 protein is stained red, & MYH11 is stained green to indicate the PASMCs (×400; scale bars indicate 50 μm). CXCR4 fluorescence intensity was calculated (b; n = 3). CXCR4 protein expression levels in lung tissue were examined by western blot. GAPDH served as an internal control (c, d; n = 3). Data are presented as the mean ± SD. #Value significantly greater than the corresponding value in saline-treated normoxic mice (##P 50 CTCs/ 2.5 ml blood) was processed via ficoll density centrifugation & the PBMC fraction was collected. Immunomagnetic anti-CD45 depletion was performed on the obtained PBMCs & the remaining cells were washed, cytospunned onto the slides. Slides were stained for PSMA, EpCAM, sLex, & CXCR4 using the protocol as described in Figure S1. MDA, PC3, & KG1 cells were simultaneously stained as a control for the following markers: PSMA= Magenta, EpCAM= Yellow, HECA-452= Green, CXCR4= Red. All prostate CTCs expressed CXCR4, while, sLex expression was variable. The analysis of sLex intensity is shown in Figure 4. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24386459), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74396_rabbit-polyclonal-cxcr4-antibody-31020241682122.jpg)
![Western Blot: CXCR4 Antibody [NB100-56437] - Western blot shows lysates of Jurkat human T lymphocyte parental cell line and CXCR4 knockout (KO) Jurkat cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human CXCR4 Polyclonal Antibody (Catalog # NB100-56437) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for CXCR4 at approximately 38 kDa (as indicated) in the parental Jurkat cell line, but is not detectable in the knockout Jurkat cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Knockdown-Validated-NB100-56437-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CXCR4 Antibody [NB100-56437] - Western blot shows lysates of Jurkat human T lymphocyte parental cell line and CXCR4 knockout (KO) Jurkat cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human CXCR4 Polyclonal Antibody (Catalog # NB100-56437) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for CXCR4 at approximately 38 kDa (as indicated) in the parental Jurkat cell line, but is not detectable in the knockout Jurkat cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: CXCR4 Antibody [NB100-56437] - Detection of 15 ug in HeLa cells with antibody concentration at 1 ug/mL.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NB100-56437-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: CXCR4 Antibody [NB100-56437] - Jurkat cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-CXCR4 (NB100-56437) at a 1:25 dilution for 1 hour at room temperature and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with and anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56437-img0003.jpg)
![Immunohistochemistry-Paraffin: CXCR4 Antibody [NB100-56437] - CXCR4 was detected in immersion sections of human tonsil using rabbit anti-human antibody (Catalog # NB100-56437) at 1:300 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-rabbit HRP polymer detection reagent with DAB chromogen (brown) and counterstained with hematoxylin (blue).](http://images.novusbio.com/fullsize/CXCR4-Antibody-Immunohistochemistry-Paraffin-NB100-56437-img0006.jpg)
![Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Brain Cerebellum Purkinje Neuron](http://images.novusbio.com/fullsize/CXCR4-Antibody-Immunohistochemistry-Paraffin-NLS1380-img0010.jpg "Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Brain Cerebellum Purkinje Neuron")
![Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Analysis of anti-CXCR4 antibody with human tonsil.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Immunohistochemistry-Paraffin-NLS1380-img0004.jpg)
![Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Analysis of anti-CXCR4 antibody with human colon, carcinoma.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Immunohistochemistry-Paraffin-NLS1380-img0006.jpg)
![Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Human Lymph Node, Non-Hodgkins Lymphoma formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Immunohistochemistry-Paraffin-NLS1380-img0007.jpg)
![Immunohistochemistry-Frozen: CXCR4 Antibody [NLS1380] - Rat brain tissue sections. Rat Nucleus Accumbens treated with MDPV. Images taken with fluorescent microscope. Primary antibody was diluted to 1:200. Secondary antibody used was donkey anti-Rabbit Alexa Fluor 594 at 1:500. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Immunohistochemistry-Frozen-NLS1380-img0008.jpg)
![Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Lung, small cell carcinoma](http://images.novusbio.com/fullsize/CXCR4-Antibody-Immunohistochemistry-Paraffin-NLS1380-img0009.jpg)
![Western Blot: CXCR4 Antibody - Azide Free [NBP2-24862] - Western blot shows lysates of Jurkat human T lymphocyte parental cell line and CXCR4 knockout (KO) Jurkat cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human CXCR4 Polyclonal Antibody (Catalog # NB100-56437) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for CXCR4 at approximately 38 kDa (as indicated) in the parental Jurkat cell line, but is not detectable in the knockout Jurkat cell line. This experiment was conducted under reducing conditions. Image using the standard format of this product.](http://images.novusbio.com/fullsize/CXCR4-Antibody---Azide-Free-Western-Blot-NBP2-24862-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CXCR4 Antibody - Azide Free [NBP2-24862] - Western blot shows lysates of Jurkat human T lymphocyte parental cell line and CXCR4 knockout (KO) Jurkat cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human CXCR4 Polyclonal Antibody (Catalog # NB100-56437) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for CXCR4 at approximately 38 kDa (as indicated) in the parental Jurkat cell line, but is not detectable in the knockout Jurkat cell line. This experiment was conducted under reducing conditions. Image using the standard format of this product.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CXCR4 Antibody - Azide Free [NBP2-24862] - Jurkat cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-CXCR4 (NB100-56437) at a 1:25 dilution for 1 hour at room temperature and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with and anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image using the standard format of this product.](http://images.novusbio.com/fullsize/CXCR4-Antibody---Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-24862-img0002.jpg)
![Immunohistochemistry: CXCR4 Antibody - Azide Free [NBP2-24862] - CXCR4 was detected in immersion sections of human tonsil using rabbit anti-human antibody (Catalog # NB100-56437) at 1:300 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-rabbit HRP polymer detection reagent with DAB chromogen (brown) and counterstained with hematoxylin (blue). Image using the standard format of this product.](http://images.novusbio.com/fullsize/CXCR4-Antibody---Azide-Free-Immunohistochemistry-NBP2-24862-img0003.jpg)
![Western Blot: CXCR4 Antibody - Azide Free [NBP2-24862] - Analysis of 15 ug of whole cell lysate from HeLa cells with anti-CXCR4 at 1 ug/ml.](http://images.novusbio.com/fullsize/CXCR4-Antibody---Azide-Free-Western-Blot-NBP2-24862-img0001.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - Loading: 15 ug of lysates per lane. Antibodies: NBP1-7706 (1 ug/mL), NBP1-76480 (1 ug/mL), and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0013.jpg "Western Blot: CXCR4 Antibody [NBP1-77067] - Loading: 15 ug of lysates per lane. Antibodies: NBP1-7706 (1 ug/mL), NBP1-76480 (1 ug/mL), and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP")
![Immunohistochemistry: CXCR4 Antibody - BSA Free [NBP1-77067] - Validation of CXCR4 in Human Spleen. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CXCR4 antibody at 5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A Goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77067_rabbit-polyclonal-cxcr4-antibody-2711202420324853.gif)
![<b>Genetic Strategies Validation. </b>Flow Cytometry: CXCR4 Antibody [NBP1-77067] - Astrocytes from wild-type or CXCR4 knockout mice were stained with primary antibodies against CXCR4 and FITClabeled secondary antibodies, and subsequently subjected to flow cytometry. CXCR4-/- astrocytes (red) showed loss of CXCR4 cell-surface expression compared with wild-type cells (black).](http://images.novusbio.com/fullsize/CXCR4-Antibody-Flow-Cytometry-NBP1-77067-img0010.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - HeLa whole cell lysate with CXCR4 antibody at 0.5 ug/ml.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0001.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - Recombinant Protein Test: Loading: CXCR4 partial recombinant protein (H00007852-Q01). Lane 1: Anti-CXCR4 antibody (0.1 ug/mL) 1 h incubation at RT in 5% NFDM/TBST. Lane 2: Coomassie blue staining.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0007.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - CXCR4 protein was detected in the human metastatic melanoma cell lines and human melanoma cell line (colo38), but not in the human primary melanocytes (MPR1) with anti-CXCR4 antibodies.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0008.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - U87MG and U87MG-CXCR4 extracts were included as negative and positive controls, respectively, for CXCR4 detection with anti-CXCR4 antibodies.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0009.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - Loading: Lysates/proteins at 20 ug per lane. Antibodies: NBP1-7706 (2 ug/mL) or NBP1-76480 (2 ug/mL). 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CXCR4 Antibody [NBP1-77067] - HeLa cells were transfected with control siRNAs (lane 1) or CXCR4 siRNAs (lane 2) Loading: 10 ug of HeLa whole cell lysates per lane. Antibodies: NBP1-7706 (2 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0012.jpg)
![Flow Cytometry: CXCR4 Antibody [NBP1-77067] - Analysis of HeLa cells using CXCR4 antibody at 0.1 ug/ml. Purple: cells without staining, Green: Isotype control. Red : CXCR4 antibody.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Flow-Cytometry-NBP1-77067-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody - BSA Free [NBP1-77067] - Validation of CXCR4 in HeLa Cells. Immunocytochemical analysis of HeLa cells using anti-CXCR4 antibody at 2 u/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77067_rabbit-polyclonal-cxcr4-antibody-2711202419313214.gif)
![Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody - BSA Free [NBP1-77067] - Validation of CXCR4 in HeLa Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling CXCR4 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on HeLa cells.](http://images.novusbio.com/fullsize/nbp1-77067_rabbit-polyclonal-cxcr4-antibody-2711202420414588.jpg)
![Western Blot: CXCR4 Antibody [NBP1-76480] - Independent Antibody Validation (IAV) via Protein Expression Profile Loading: 15 ug of lysates per lane. Antibodies: NBP1-77067 (1 ug/mL), NBP1-76480 (1 ug/mL), and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-76480-img0009.jpg "<b>Independent Antibodies Validation. </b>Western Blot: CXCR4 Antibody [NBP1-76480] - Independent Antibody Validation (IAV) via Protein Expression Profile Loading: 15 ug of lysates per lane. Antibodies: NBP1-77067 (1 ug/mL), NBP1-76480 (1 ug/mL), and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST.")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody - BSA Free [NBP1-76480] - Figure 5 Immunofluorescence Validation of CXCR4 Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling CXCR4 with at 4 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on HeLa cell line.](http://images.novusbio.com/fullsize/nbp1-76480_rabbit-polyclonal-cxcr4-antibody-2711202419562119.jpg)
![Immunohistochemistry-Paraffin: CXCR4 Antibody [NBP1-76480] - Staining of CXCR4 in mouse spleen tissue with CXCR4 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Immunohistochemistry-Paraffin-NBP1-76480-img0006.jpg)
![Western Blot: CXCR4 Antibody [NBP1-76480] - HeLa total cell lysate.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-76480-img0002.jpg)
![Western Blot: CXCR4 Antibody [NBP1-76480] - Animal Species ReactivityLoading: Lysates/proteins at 20 ug per lane.Antibodies: NBP1-77067 (2 ug/mL) or NBP1-76480 (2 ug/mL). 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-76480-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CXCR4 Antibody [NBP1-76480] - Validation with CXCR4 siRNA Knockdown. HeLa cells were transfected with control siRNAs (lane 1) or CXCR4 siRNAs (lane 2) Loading: 10 ug of HeLa whole cell lysates per lane. Antibodies: NBP1-76480 (2 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Knockdown-Validated-NBP1-76480-img0008.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77560] - Analysis of CXCR4 in (A) HeLa and (B) A431 cell lysate.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77560-img0001.jpg "Western Blot: CXCR4 Antibody [NBP1-77560] - Analysis of CXCR4 in (A) HeLa and (B) A431 cell lysate.")
![Simple Western: CXCR4 Antibody [NBP1-77560] - Lane view shows a specific band for CXCR4 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Simple-Western-NBP1-77560-img0002.jpg)