Osteocalcin: Novus Biologicals

MAB1419

Osteocalcin Antibody (190125)

(3 Reviews)

100 μg

MAB1419

NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

Osteocalcin was detected in immersion fixed MG-63 human osteosarcoma cell line using Mouse Anti-Human/Rat Osteocalcin Monoclonal Antibody (Catalog # MAB1419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="Osteocalcin was detected in immersion fixed MG-63 human osteosarcoma cell line using Mouse Anti-Human/Rat Osteocalcin Monoclonal Antibody (Catalog # MAB1419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

Osteocalcin was detected in human mesenchymal stem cells differentiated into osteocytes using Mouse Anti-Human/Rat Osteocalcin Monoclonal Antibody (Catalog # MAB1419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

Osteocalcin was detected in immersion fixed rat osteocytes differentiated from mesenchymal stem cells using Mouse Anti-Human/Rat Osteocalcin Monoclonal Antibody (Catalog # MAB1419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of chondrocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Osteocalcin was detected in immersion fixed paraffin-embedded sections of human cartilage using Mouse Anti-Human/Rat Osteocalcin Monoclonal Antibody (Catalog # MAB1419) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of chondrocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Osteocalcin was detected in immersion fixed paraffin-embedded sections of human osteosarcoma using Mouse Anti-Human/Rat Osteocalcin Monoclonal Antibody (Catalog # MAB1419) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_lightbox">

Saos-2 human osteosarcoma cell line was stained with Mouse Anti-Human/Rat Osteocalcin Monoclonal Antibody (Catalog # MAB1419, filled histogram) or isotype control antibody (Catalog # <a class=

MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_thumb" />

MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_lightbox">

Human osteoblasts were stained with Mouse Anti-Human/Rat Osteocalcin Monoclonal Antibody (Catalog # MAB1419, filled histogram) or isotype control antibody (Catalog # <a class=

MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_thumb" />

Exposure of TAZ activator TM-25659 promotes osteogenic differentiation of ADSCs in vitro. a Cell viability was determined after ADSCs were cultured with diverse concentrations of TM-25659 (0–50 μM) at 24 h and 48 h, respectively. B Enhanced osteogenic differentiation of ADSCs was detected and quantified by alkaline phosphatase (ALP) activity assay following ADSC culture with osteoinductive medium and TM-25659 at day 7. c, d Increased mineralization in ADSCs cultured in osteoinductive medium and TM-25659 was observed and quantified via Alizarin Red staining at days 7 and 14. Scale bar = 50 μm. e Significantly increased expression of the osteogenic markers runt-related transcription factor 2 (Runx2), osteopontin (OPN), and osteocalcin (OCN) was observed in ADSCs cultured in osteoinductive medium and TM-25659 at days 7 and 14, respectively. Representative images of Western blots from three independent experiments are shown. Data shown here are mean ± SD from three independent experiments; **P < 0.01, by Student’s t test. GAPDH glyeraldehyde-3-phosphate dehydrogenase, OD optical density Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29514703), licensed under a CC-BY license. Not internally tested by R&D Systems.

Nano-fiber plugs induce osteogenesis of human MSCs.Healthy donor-derived MSCs were seeded onto plastic plates (A-C) or injected into the nano-fiber plugs (D-I), and then cultured in MSCGM for 1 day (D), 7 days (E, G), 14 days (F, H) and 28 days (A-C, I). Then, the expression levels of RUNX2 (A, D, G), osteocalcin (B, E, H), and DMP-1 (C, F, I) were evaluated immunohistochemically. Representative results of three experiments are shown. (J) Healthy donor-derived MSCs were cultured in MSCGM onto plastic plates (two-dimensional) or nano-fiber plugs (three-dimensional) for 28 days. After RNA extraction, MEPE expression in the 4 groups of healthy donor-derived MSCswas evaluated by real-time PCR. *p<0.05 vs. two-dimensional culture by paired t-test. Scale bars, 50 μm. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0153231), licensed under a CC-BY license. Not internally tested by R&D Systems.

Enforced TAZ overexpression enhances osteogenic differentiation of ADSCs in vitro. a Transcriptional coactivator with PDZ-binding motif (TAZ) overexpression (OP) in ADSCs infected with lentiviral particles containing human TAZ cDNA sequence was verified by Western blot. Representative images of Western blots from three independent experiments are shown. b Accelerated cell proliferation was detected in TAZ-overexpressing ADSCs compared with control cells infected with empty lentiviral particles via MTT assay. c Enhanced osteogenic differentiation of ADSCs was detected and quantified by alkaline phosphatase (ALP) activity assay following TAZ overexpression in ADSCs induced in vitro at day 7. d Increased mineralization in TAZ-overexpressing ADSCs cultured in osteoinductive medium was observed via Alizarin Red staining at days 7 and 14. Scale bar = 50 μm. e Significantly enhanced expression of TAZ as well as the osteogenic markers osteopontin (OPN) and osteocalcin (OCN) in TAZ-overexpressing ADSCs cultured in osteoinductive medium at days 7, 14, and 21 was detected by Western blot. Representative images of Western blots from three independent experiments are shown. Data shown here are mean ± SD from three independent experiments; #P ˃ 0.05, *P < 0.05, **P < 0.01, by Student’s t test. GAPDH glyeraldehyde-3-phosphate dehydrogenase, OD optical density, Runx2 runt-related transcription factor 2 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29514703), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Genetic Strategies Validation. </b>TAZ knockdown impairs osteogenic differentiation of ADSCs in vitro. a Transcriptional coactivator with PDZ-binding motif (TAZ) knockdown (KD) in ADSCs infected with shRNA lentiviral particles targeting human TAZ was verified by Western blot. Representative images of Western blots from three independent experiments are shown. b TAZ and its downstream targets CTGF and Cyr61 were downregulated in TAZ-knockdown ADSCs as determined by quantitative RT-PCR. c Cell proliferation was compromised in TAZ-knockdown ADSCs compared with control cells infected with nontargeting lentiviral vectors with scrambled sequence. d Compromised osteogenic differentiation of ADSCs was detected and quantified by alkaline phosphatase (ALP) activity assay following TAZ knockdown of ADSCs induced in vitro at day 7. e Reduced mineralization in TAZ-knockdown ADSCs cultured in osteoinductive medium was observed via Alizarin Red staining at days 7, 14, and 21. f Significantly reduced expression of TAZ as well as the osteogenic markers osteopontin (OPN) and osteocalcin (OCN) in TAZ-knockdown ADSCs cultured in osteoinductive medium at days 7, 14, and 21 was detected by Western blot. Representative images of Western blots from three independent experiments are shown. Data shown here are mean ± SD from three independent experiments, #P ˃ 0.05, *P < 0.05, **P < 0.01, by Student’s t test. GAPDH glyeraldehyde-3-phosphate dehydrogenase, NC normal control, OD optical density, Runx2 runt-related transcription factor 2 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29514703), licensed under a CC-BY license. Not internally tested by R&D Systems.

TAZ is upregulated during osteogenesis while it is downregulated during adipogenesis in human ADSCs. a Osteogenic differentiation of ADSCs was induced in vitro and monitored by Alizarin Red and von Kossa staining at the indicated time points (days 7, 14, 21, and 28 after induction). Scale bar = 50 μm. b,c Transcriptional coactivator with PDZ-binding motif (TAZ) protein was significantly increased during the osteogenic differentiation process of ADSCs, concomitant with markedly upregulated expression of the osteogenic markers runt-related transcription factor 2 (Runx2), osteopontin (OPN), and osteocalcin (OCN). Representative images of Western blots from three independent experiments are shown. d The mRNA levels of TAZ and the osteogenic markers were significantly increased during ADSC osteogenic differentiation in vitro as measured by quantitative RT-PCR. e Adipogenic differentiation of ADSCs was induced in vitro and determined by Oil Red O staining at the indicated time points (days 7, 14, and 21 after induction). Scale bar = 50 μm. f TAZ protein was significantly downregulated during the adipogenic differentiation process of ADSCs. Representative images of Western blots from three independent experiments are shown. g The mRNA levels of TAZ decreased while the adipogenic marker increased during ADSC adipogenic differentiation in vitro as measured by quantitative RT-PCR. Data shown here are mean ± SD from three independent experiments; #P ˃ 0.05, *P < 0.05, **P < 0.01, by ANOVA. ALP alkaline phosphatase, GAPDH glyeraldehyde-3-phosphate dehydrogenase, PPAR gamma peroxisome proliferator-activated receptor-gamma Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29514703), licensed under a CC-BY license. Not internally tested by R&D Systems.

In vitro differentiation capacity of young and old BM-MSCs: (A) Representative micrographs from each experimental group showing morphological change after 21 days of differentiation. (B) Results of adipogenic, osteogenic and chondrogenic differentiation. Representative micrographs showing anti-FABP4, Oil Red O, anti-osteocalcin, Alizarin Red-S and anti-aggrecan staining in cultured BM-MSCs from each experimental group. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21992089), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>TM-25659 promotes in vivo bone formation of ADSCs. a Schematic description of experimental procedure for in vivo transplantation. Adipose-derived stem cells (ASCs) were initially treated with osteoinductive medium and TM-25659 for consecutive 7 days, then harvested, seeded on porous beta -TCP blocks as a carrier, and subcutaneously transplanted into nude mice (six animals per experimental group). Six weeks later all transplants were harvested for further analysis. b Hematoxylin and eosin (H&E) and Masson trichrome staining revealed markedly enhanced bone formation in samples from ADSCs treated with TM-25659 compared with vehicle-treated samples. Scale bar = 100 μm. c Quantification of bone formation in samples indicated significantly more bone formation in ADSCs treated with TM-25659. Ten images of Masson staining (400×) were randomly selected in the slides from two experimental groups and captured under microscopy. The area of new bone in each image was marked using ImageJ and the percentage of new bone over total area was calculated. d Immunohistochemical staining of osteopontin (OPN) and osteocalcin (OCN) in samples revealed elevated OPN and OCN abundance in samples from ADSCs treated with TM-25659 compared with vehicle-treated samples. Scale bar = 100 μm. Data are shown as fold-change compared with vehicle-treated samples which were defined as 1.0. Data shown here are mean ± SD from two independent experiments; **P < 0.01, by Student’s t test. NB new bone, S scaffold Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29514703), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>TM-25659 delivery by oral gavage promotes in vivo bone formation of ADSCs. a Schematic description of the experimental procedure for in vivo transplantation. Adipose-derived stem cells (ASCs) were initially treated with osteoinductive medium for 7 consecutive days and then harvested, seeded on porous beta -TCP blocks as carriers, and subcutaneously transplanted into nude mice (six animals per experimental group). Six weeks later all transplants were harvested for further analysis. b Hematoxylin and eosin (H&E) and Masson trichrome staining revealed markedly enhanced bone formation in samples from animals treated with TM-25659 via oral gavage compared with vehicle-treated samples. Scale bar = 100 μm. c Quantification of bone formation in samples indicated significantly more bone formation in samples from animals treated with TM-25659. Ten images of Masson staining (400×) were randomly selected in the slides from two experimental groups and captured under microscopy. The area of new bone in each image was marked using ImageJ and the percentage of new bone over total area was calculated. d Immunohistochemical staining of osteopontin (OPN) and osteocalcin (OCN) in samples revealed elevated OPN and OCN abundance in samples from animals treated with TM-25659 compared with vehicle-treated samples. Scale bar = 100 μm. Data are shown as fold-change compared with vehicle-treated samples which were defined as 1.0. Data shown here are mean ± SD from two independent experiments; *P < 0.05, **P < 0.01, by Student’s t test. NB new bone, S scaffold Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29514703), licensed under a CC-BY license. Not internally tested by R&D Systems.

Msx1+ SSCs subset is a source of osteogenesis and closely correlates with endochondral ossification.a Schematic of the assessment of Msx1+ SSCs subset in differentiation hierarchy by Prx1-lineage tracing mice. b, c HE staining of newly formed calvarium tissues in skull bone injury at 2 weeks and 4 weeks after surgery, respectively. d–g Coronal sections of the calvarium tissues from Prx1Cre; RosatdTomato mice co-immunostained with Msx1 and Runx2 (d); Msx1 and OCN (e); Msx1 and ACAN (f); Msx1 and COL2 (g); Scale bars: low magnification: 30 μm, high magnification: 10 μm. At least three times of experiments were repeated independently. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36064711), licensed under a CC-BY license. Not internally tested by R&D Systems.

3D printed hydrogel scaffold with NSs enhances in vitro expansion and osteogenic potential of MSCs.a Schematic diagram of NSs-loaded 3D printing gel scaffold by DLP technology. b Bright field image of MSCs distribution and cell morphology on the surface and inside of the gel scaffold and NSs-loaded gel scaffold for 3 days and 7 days, bar = 200 μm. c Live/Dead staining (Green, live cells; Red, dead cells) of MSCs expanded on gel scaffold and NSs-loaded gel scaffold for 7 days and 14 days, bar = 50 μm. d Bright field image and Live/Dead staining (bar = 200 μm, 50μm); e Gross view and f ALP staining, bar = 100μm, Exact p value calculated with unpaired t-test: ***p = 0.0003, n = 5 biologically independent samples; g immunostaining of RUNX2 and OCN after 7 days’ osteogenic differentiation of expanded MSCs on gel scaffold after 7 days (bar = 30 μm). h Bright field image and Live/Dead staining (bar = 200 μm, 50 μm); i Gross view and j Alizarin Red S staining, bar = 100 μm, Exact p value calculated with unpaired t-test: ***p = 0.0004, n = 4 biologically independent samples; k immunostaining of RUNX2 and OCN after 14 days’ osteogenic differentiation of expanded MSCs on gel scaffold after 7 days (bar = 30 μm). Bone marrow stem cells are isolated from orthopedic individuals with written informed consent. All data represent mean ± SEM, and source data are provided as a Source Data file. At least three times of experiments were repeated independently. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36064711), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC1419U-100UG
Alexa Fluor 405	IC1419V-100UG
Alexa Fluor 488	IC1419G
Alexa Fluor 532	FAB1419X
Alexa Fluor 594	IC1419T-100UG
Alexa Fluor 647	IC1419R-100UG
Alexa Fluor 700	IC1419N-100UG
Alexa Fluor 750	IC1419S-100UG
Alexa Fluor Plus 405	FAB1419AFP405-100UG
Alexa Fluor Plus 488	FAB1419AFP488-100UG
Alexa Fluor Plus 555	FAB1419AFP555-100UG
Alexa Fluor Plus 594	FAB1419AFP594-100UG
Alexa Fluor Plus 647	FAB1419AFP647-100UG
Alexa Fluor Plus 680	FAB1419AFP680-100UG
Allophycocyanin	IC1419A
Biotin	FAB1419B
CoraFluor 1	FAB1419CL1
DyLight 350	FAB1419D
DyLight 405	FAB1419E
DyLight 488	FAB1419K
DyLight 550	FAB1419L
DyLight 594	FAB1419M
DyLight 650	FAB1419W
DyLight 680	FAB1419Y
DyLight 755	FAB1419Z
FITC	FAB1419F
HRP	FAB1419H
Janelia Fluor 525	FAB1419JF525
Janelia Fluor 549	FAB1419I
Janelia Fluor 646	FAB1419J
Janelia Fluor 669	FAB1419JF669
PerCP	FAB1419C
Phycoerythrin	IC1419P
mFluor Violet 450 SE	FAB1419MFV450
mFluor Violet 500 SE	FAB1419MFV500
mFluor Violet 610 SE	FAB1419MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC1419P
Carrier Free	IC1419A
Carrier Free	MAB1419-SP
Carrier Free	IC1419G
Carrier Free	IC1419T-100UG
Carrier Free	IC1419S-100UG
Carrier Free	IC1419R-100UG
Carrier Free	IC1419N-100UG
Carrier Free	IC1419V-100UG
Carrier Free	IC1419U-100UG

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NBP2-68151

Mouse Osteocalcin ELISA Kit (Colorimetric)

(14 Publications)

1 Kit

NBP2-68151

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Standard Curve:

0.39 - 25 ng/mL

Sensitivity:

0.23 ng/mL

Sample Type:

Serum, plasma and other biological fluids

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NBP2-68153

Rat Osteocalcin ELISA Kit (Colorimetric)

(8 Publications)

1 Kit

NBP2-68153

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

Applications:

Standard Curve:

0.78-50 ng/mL

Sensitivity:

0.47 ng/mL

Sample Type:

Serum, plasma and other biological fluids

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DY1419-05

Human Osteocalcin DuoSet ELISA, 5 Plate

(13 Publications)

1 Kit (for 5 Plates)

DY1419-05

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NBP3-16081

Osteocalcin Antibody (2T1J7)

100 ul

NBP3-16081

Immunocytochemistry/ Immunofluorescence: Osteocalcin Antibody (2T1J7) [NBP3-16081] - Immunofluorescence analysis of NIH/3T3 cells using Osteocalcin Rabbit mAb at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP2-68152

Monkey Osteocalcin ELISA Kit (Colorimetric)

1 Kit

NBP2-68152

Reactivity:

Pm

Species Glossary

Reactivity: Pm

Applications:

Applications:

Standard Curve:

0.78-50 ng/mL

Sensitivity:

0.47 ng/mL

Sample Type:

Serum, plasma and other biological fluids

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NBP2-77048

Canine Osteocalcin ELISA Kit (Colorimetric)

1 Kit

NBP2-77048

Reactivity:

Ca

Species Glossary

Reactivity: Ca

Applications:

Applications:

Standard Curve:

0.312 ng/mL - 20 ng/mL

Sensitivity:

0.078 ng/mL

Sample Type:

Serum, plasma and tissue homogenates

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H00000632-M01

Osteocalcin Antibody (2D4) - Azide and BSA Free

(3 Publications)

0.1 mg

H00000632-M01

ELISA: Osteocalcin Antibody (2D4) [H00000632-M01] - Detection limit for recombinant GST tagged BGLAP is approximately 0.3ng/ml as a capture antibody.

h00000632-m01_mouse-monoclonal-osteocalcin-antibody-2d4-25520231539515.jpg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP3-11788

Human Undercarboxylated Osteocalcin ELISA Kit (Colorimetric)

(1 Publication)

1 Kit

NBP3-11788

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Standard Curve:

6.25 - 400 pg/mL

Sensitivity:

3.75 pg/mL

Sample Type:

Serum, plasma and other biological fluids

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H00000632-P01

Recombinant Human Osteocalcin GST (N-Term) Protein

(2 Publications)

10 μg

H00000632-P01

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

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MAB113272

Osteocalcin Antibody (582014) [Unconjugated]

100 μg

MAB113272

MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004). View our protocol for Staining Intracellular Molecules." class="big_lightbox">

MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004). View our protocol for Staining Intracellular Molecules." alt="SAOS-2 cells were stained with Mouse Anti-Human Osteocalcin Monoclonal Antibody (Catalog # MAB113272, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC113272U-100UG
Alexa Fluor 405	IC113272V-100UG
Alexa Fluor 488	IC113272G-100UG
Alexa Fluor 532	FAB113272X
Alexa Fluor 594	IC113272T-100UG
Alexa Fluor 647	IC113272R-100UG
Alexa Fluor 700	IC113272N-100UG
Alexa Fluor 750	IC113272S-100UG
Alexa Fluor Plus 405	FAB113272AFP405
Alexa Fluor Plus 488	FAB113272AFP488
Alexa Fluor Plus 555	FAB113272AFP555
Alexa Fluor Plus 594	FAB113272AFP594
Alexa Fluor Plus 647	FAB113272AFP647
Alexa Fluor Plus 680	FAB113272AFP680
Allophycocyanin	FAB113272A
Biotin	FAB113272B
CoraFluor 1	FAB113272CL1
DyLight 350	FAB113272D
DyLight 405	FAB113272E
DyLight 488	FAB113272K
DyLight 550	FAB113272L
DyLight 594	FAB113272M
DyLight 650	FAB113272W
DyLight 680	FAB113272Y
DyLight 755	FAB113272Z
FITC	FAB113272F
HRP	FAB113272H
Janelia Fluor 525	FAB113272JF525
Janelia Fluor 549	FAB113272I
Janelia Fluor 646	FAB113272J
Janelia Fluor 669	FAB113272JF669
PE	FAB113272P
PerCP	FAB113272C
mFluor Violet 450 SE	FAB113272MFV450
mFluor Violet 500 SE	FAB113272MFV500
mFluor Violet 610 SE	FAB113272MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC113272U-100UG
Carrier Free	IC113272T-100UG
Carrier Free	IC113272G-100UG
Carrier Free	IC113272N-100UG
Carrier Free	MAB113272-SP
Carrier Free	IC113272V-100UG
Carrier Free	IC113272S-100UG
Carrier Free	IC113272R-100UG

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MAB11327

Osteocalcin Antibody (582020) [Unconjugated]

100 μg

MAB11327

DY990). Mouse Anti-Human Osteocalcin Monoclonal Antibody (MAB113271) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox">

Recombinant Human Osteocalcin protein was serially diluted 2-fold and captured by Mouse Anti-Human Osteocalcin Monoclonal Antibody (Catalog # MAB11327) coated on a Clear Polystyrene Microplate (Catalog # <a class=

DY990). Mouse Anti-Human Osteocalcin Monoclonal Antibody (MAB113271) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human Osteocalcin protein was serially diluted 2-fold and captured by Mouse Anti-Human Osteocalcin Monoclonal Antibody (Catalog # MAB11327) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Osteocalcin Monoclonal Antibody (MAB113271) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB11327U-100UG
Alexa Fluor 405	FAB11327V-100UG
Alexa Fluor 488	FAB11327G-100UG
Alexa Fluor 532	FAB11327X-100UG
Alexa Fluor 594	FAB11327T-100UG
Alexa Fluor 647	FAB11327R-100UG
Alexa Fluor 700	FAB11327N-100UG
Alexa Fluor 750	FAB11327S-100UG
Alexa Fluor Plus 405	FAB11327AFP405-100UG
Alexa Fluor Plus 488	FAB11327AFP488-100UG
Alexa Fluor Plus 555	FAB11327AFP555-100UG
Alexa Fluor Plus 594	FAB11327AFP594-100UG
Alexa Fluor Plus 647	FAB11327AFP647-100UG
Alexa Fluor Plus 680	FAB11327AFP680-100UG
Allophycocyanin	FAB11327A
Biotin	FAB11327B
CoraFluor 1	FAB11327CL1
DyLight 350	FAB11327D
DyLight 405	FAB11327E
DyLight 488	FAB11327K
DyLight 550	FAB11327L
DyLight 594	FAB11327M
DyLight 650	FAB11327W
DyLight 680	FAB11327Y
DyLight 755	FAB11327Z
FITC	FAB11327F
HRP	FAB11327H
Janelia Fluor 525	FAB11327JF525
Janelia Fluor 549	FAB11327I
Janelia Fluor 646	FAB11327J
Janelia Fluor 669	FAB11327JF669
PE	FAB11327P
PerCP	FAB11327C
mFluor Violet 450 SE	FAB11327MFV450
mFluor Violet 500 SE	FAB11327MFV500
mFluor Violet 610 SE	FAB11327MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB11327-SP

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Kit Type

Preservative

Research Areas

Has Sample Size

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