IL-1 beta/IL-1F2: Novus Biologicals

MAB201

IL-1 beta/IL-1F2 Antibody (8516) [Unconjugated]

100 μg

MAB201

HAF018). A specific band was detected for IL-1 beta /IL-1F2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS and 3 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB201) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF018). A specific band was detected for IL-1 beta /IL-1F2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS and 3 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB201) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-1 beta /IL-1F2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

<b>Biological Strategies Validation. </b>IL‑1 beta /IL‑1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB201) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

<b>Biological Strategies Validation. </b>IL-1 beta /IL-1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with LPS and monensin using Human IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB201) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # <a class=

NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

<b>Biological Strategies Validation. </b>Simple Western lane view shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nm PMA and 10 ug/ml LPS for 24 hrs and 3 hrs, respectively, loaded at 0.2 mg/mL. A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 38 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB201) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

201-LB) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1 beta /IL-1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB201). The ND₅₀ is typically 0.001-0.003 µg/mL." class="big_lightbox">

Recombinant Human IL-1 beta /IL-1F2 (Catalog # <a class=

201-LB) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1 beta /IL-1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB201). The ND₅₀ is typically 0.001-0.003 µg/mL." class="big_thumb" />

<b>Biological Strategies Validation. </b>Hsp27 modulates IL-1 beta production in monocytes.(a) Immunoblot analysis of Hsp27 protein expression in classical (CL) and non-classical (NC) monocytes (left), and relative band densities from 2 combined experiments (right), with GAPDH as loading control. (b) Representative immunoblot of Hsp27 and pro-IL-1 beta (p31) in lysates from siRNA-transfected total monocytes for si-control (scrambled control siRNA) or si-Hsp27 (siRNA to Hsp27) either untreated or stimulated with LPS for 3 h. Data in (a) and (b) are cropped images and the corresponding full-length images are presentated in Supplementary Fig. 3. (c) Expression of IL-1 beta mRNA in siRNA-transfected total monocytes (n = 3). (d) Mature IL-1 beta release measured by ELISA of supernatants from siRNA-transfected total monocytes upon LPS stimulation in the presence or absence of BzATP (n = 4). (e) Fold change in IL-1 beta production by LPS and BzATP-treated total monocytes transfected with si-Hsp27, with respect to si-control. Data plotted are mean ± SEM, student t-test (a and b) and two-way ANOVA with Tukey’s multiple comparison test (c), *p < 0.05, **p < 0.01. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27976724), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Non-classical monocytes secrete less mature IL-1 beta compared to other monocyte subsets following LPS and BzATP stimulation.(a) IL-1 beta ELISA on supernatants from monocyte subsets either untreated or stimulated with LPS for 24 h in the presence or absence of the P2X7 antagonist, A438079, followed by a secondary stimulation by BzATP in some conditions as indicated. Data plotted are mean ± SEM; n = 8. ****p < 0.0001 ***p < 0.001. ##p < 0.01 and #p < 0.05 is comparison to the respective classical and intermediate subsets treated with LPS + BzATP. Statistics are calculated based on Two-way ANOVA with Tukey’s multiple comparison test (*p < 0.05). (b) Representative cropped immunoblot images for mature 17 kDa (p17) IL-1 beta in culture supernatants of monocyte subsets stimulated as described in (a). The samples were derived from the same experiment and were ran on the same gel. Full-length images are shown in Supplementary Fig. 3. (c) YoPro-1 dye uptake was measured by flow cytometry to reflect level of P2X7R activity, and is shown as the fold change of BzATP-induced YoPro-1 uptake normalized to the respective untreated subset uptake. Data plotted are mean ± SEM; n = 6 based on one-way ANOVA with Tukey’s multiple comparison test. CL: classical, INT: intermediate, NC: non-classical. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27976724), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry for CD3, IL-1 beta and CD79 expression.Some expression for these markers were found in the CS and CSVT groups at 21 days (positive signal by brown staining). The same overall expression to the materials was seen at the other time points. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0136514), licensed under a CC-BY license. Not internally tested by R&D Systems.

Yptb effectors enable evasion of T3SS-dependent inflammasome activation in human cells. BMDMs (A), Caco-2 cells (B, D, and E), or primary hMDMs from three healthy human donors (C, F, and G) were infected with PBS (mock), WT Yptb, or delta 6 Yptb. (A–C) Cell death was measured as percentage of cytotoxicity normalized to cells treated with 2% triton at 6 hpi. (D) Release of IL-18 into the supernatant was measured by ELISA at 6 hpi. (E) Lysates and supernatants collected 6 hpi were immunoblotted for IL-18 and beta -actin. (F) Release of IL-1 beta into the supernatant was measured by ELISA at 6 hpi. (G) Lysates and supernatants collected 6 hpi were immunoblotted for IL-1 beta and beta -actin. ns: not significant; *P < 0.05; **P < 0.01 by t-test (A–C) or one-way ANOVA (D and F). Shown are averages and error bars representing the standard deviation from at least three pooled experiments. ANOVA, analysis of variance; BMDMs, bone marrow-derived macrophages; ELISA, enzyme-linked immunosorbent assay; PBS, phosphate-buffered saline. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37615436), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC201U-100UG
Alexa Fluor 405	IC201V-100UG
Alexa Fluor 488	IC201G-100UG
Alexa Fluor 532	FAB201X
Alexa Fluor 594	IC201T-100UG
Alexa Fluor 647	IC201R-100UG
Alexa Fluor 700	IC201N-100UG
Alexa Fluor 750	IC201S-100UG
Alexa Fluor Plus 405	FAB201AFP405-100UG
Alexa Fluor Plus 488	FAB201AFP488-100UG
Alexa Fluor Plus 555	FAB201AFP555-100UG
Alexa Fluor Plus 594	FAB201AFP594-100UG
Alexa Fluor Plus 647	FAB201AFP647-100UG
Alexa Fluor Plus 680	FAB201AFP680-100UG
Allophycocyanin	IC201A
Biotin	FAB201B
CoraFluor 1	FAB201CL1
DyLight 350	FAB201D
DyLight 405	FAB201E
DyLight 488	FAB201K
DyLight 550	FAB201L
DyLight 594	FAB201M
DyLight 650	FAB201W
DyLight 680	FAB201Y
DyLight 755	FAB201Z
Fluorescein	IC201F
HRP	FAB201H
Janelia Fluor 525	FAB201JF525
Janelia Fluor 549	FAB201I
Janelia Fluor 646	FAB201J
Janelia Fluor 669	FAB201JF669
Phycoerythrin	IC201P
mFluor Violet 450 SE	FAB201MFV450
mFluor Violet 500 SE	FAB201MFV500
mFluor Violet 610 SE	FAB201MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC201F
Carrier Free	IC201P
Carrier Free	IC201A
Carrier Free	MAB201-SP
Carrier Free	MAB201-500
Carrier Free	IC201V-100UG
Carrier Free	IC201U-100UG
Carrier Free	IC201T-100UG
Carrier Free	IC201S-100UG
Carrier Free	IC201R-100UG
Carrier Free	IC201N-100UG
Carrier Free	IC201G-100UG

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MAB601

IL-1 beta/IL-1F2 Antibody (2805) [Unconjugated]

(1 Review)

(64 Publications)

100 μg

MAB601

HAF018). A specific band was detected for IL-1 beta /IL-1F2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

HAF018). A specific band was detected for IL-1 beta /IL-1F2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS and 3 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB601) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-1 beta /IL-1F2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm of THP-1 cells treated with 200nM PMA for 24 hours then 10ug/mL LPS for 24 hours. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

<b>Biological Strategies Validation. </b>IL‑1 beta /IL‑1F2 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line using Mouse Anti-Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB601) at 25 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm of THP-1 cells treated with 200nM PMA for 24 hours then 10ug/mL LPS for 24 hours. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

IL-1 beta /IL-1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB601) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

201-LB) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1 beta /IL-1F2 (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB601). The ND₅₀ is typically 0.05-0.2 µg/mL." class="big_lightbox">

201-LB) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1 beta /IL-1F2 (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB601). The ND₅₀ is typically 0.05-0.2 µg/mL." class="big_thumb" />

Effect of A1AT on whole blood IL-1 beta release. IL-1 beta production in whole blood cultures in response to LPS (1.0 μg/ml) was performed in the presence of endogenous A1AT (i.e., undiluted) or exogenously added A1AT (2 mg/ml) in blood diluted 1:32 with RPMI. Whole blood cultures were incubated for 18 h. After incubation, plasma supernatants were removed, and IL-1 beta quantified by ELISA and expressed as mean ± SD for three donors. The diluted sample result was corrected for the dilution. NS indicates no significant difference. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0117330), licensed under a CC-BY license. Not internally tested by R&D Systems.

Inflammatory response induced by HI injury and maternal SE. Representative images of immunofluorescence staining of inflammatory cytokines IL-1 beta , IL-6, and TNF alpha in the cerebral cortex (A) and the hippocampus (E). The immunofluorescence intensity of IL-1 beta (B,F), IL-6 (C,G), and TNF alpha (D,H) in the cerebral cortex and hippocampus. Results are presented as mean ± SEM. *P < 0.05, **P < 0.01, n = 4, analyzed by two-way ANOVA followed by post hoc Turkey tests. SH, from sham exposed dams with sham surgery; HI, hypoxic-ischemic injury; SE, from smoke exposed dams with sham surgery; HI + SE, from smoke exposed dams with hypoxic-ischemic injury. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35250486), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Effect of high glucose and IL-1 beta alone or in combination on the protein expressions of CD31, FSP1 and alpha -SMA in HAECs. a–d HAECs were incubated for 48 h with NG and HG. Mannitol was used as a control for hyperosmolarity. Representative western blots (a) and quantitative determinations of CD31, FSP1 and alpha -SMA protein levels (b–d) are presented. e–h HAECs were treated for 48 h with NG, HG, IL-1 beta (10 ng/ml)and HG in the presence of the IL-1 beta (10 ng/ml). Representative western blots (E) and quantitative determinations of CD31, FSP1 and alpha -SMA protein levels (f–h) are presented. The data are expressed as the mean ± SD. Experiments were repeated at least three times. NG normal glucose (5.5 mM), HG high glucose (30 mM), MN 5.5 mM glucose + 24.5 mM mannitol, IL-1 beta (10 ng/ml), HG + IL-1 beta : high glucose (30 mM) + IL-1 beta (10 ng/ml) *P < 0.05 vs. MN or NG, **P < 0.01 vs. NG, #P < 0.05 vs. HG Image collected and cropped by CiteAb from the following publication (//www.cardiab.com/content/15/1/42), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>The influence of high glucose or IL-1 beta on immunofluorescence of CD31 and FSP1 in HAECs. Representative immunofluorescence images showing CD31 (green), FSP1 (red) labeling and DAPI (blue) stains nuclei. a Normal ECs monolayers displayed a cobble stone morphology. b A merge of the three images revealed some cells populations that acquired a spindle-shaped morphology and lost CD31 expression (white arrow). c HAECs exposure to IL-1 beta alone for 48 h acquired a spindle-shaped morphology. d High glucose and IL-1 beta in combination resulted in decreased CD31 (the left white arrow) and increased FSP1staining (the right arrow). a normal glucose (5.5 mM) group, b high glucose (30 mM) group for 48 h; c treatment with a normal glucose (5.5 mM) + IL-1 beta (10 ng/ml) treatment for 48 h, d treatment with a high glucose (30 mM) + IL-1 beta (10 ng/ml) treatment for 48 h. Scale bar, 75 μm Image collected and cropped by CiteAb from the following publication (//www.cardiab.com/content/15/1/42), licensed under a CC-BY license. Not internally tested by R&D Systems.

Effects of PKC beta on high glucose induced IL-1 beta up-regulation. Confluent cultures of HAECs were exposed to NG, HG, PMA (30 nM) and HG in the presence of the selective PKC beta inhibitors (LY317615, 0.3 μM) for 48 h. Real-time PCR analyses showed mRNA expression of PKC beta and IL-1 beta (a, b). Representative western blots (c) and quantitative determinations of PKC beta and IL-1 beta (d, e) are presented. The data are expressed as the mean ± SD. Experiments were repeated at least three times. NG normal glucose (5.5 mM), HG high glucose (30 mM), PMA (30 nM): phorbol 12-myristate13-acetate; LY (0.3 uM): LY317615; *P < 0.05 vs.NG, **P < 0.01 vs. NG, #P < 0.05 vs. HG or PMA Image collected and cropped by CiteAb from the following publication (//www.cardiab.com/content/15/1/42), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>The influence of blocking IL-1 beta treatment on the protein expressions of CD31, FSP1, a-SMA, and IL-1 beta . (a–f) HAECs were incubated for 48 h with anti-IL-1 beta antibodies (1000 ng/ml) in the presence of NG or HG. (a1–f1) We performed gene-silencing experiments using transfection with siRNA specific for IL-1 beta . The protein expressions of IL-1 beta , CD31, FSP1 and alpha -SMA were assessed by western blotting. The data are expressed as the mean ± SD. Experiments were repeated at least three times. NG normal glucose (5.5 mM), HG high glucose (30 mM). Anti-IL-1 beta : anti-IL-1 beta antibodies (1000 ng/ml). *P < 0.05 vs. NG or anti-IL-1 beta , #P < 0.05 vs. HG or HG +Vehicle Image collected and cropped by CiteAb from the following publication (//www.cardiab.com/content/15/1/42), licensed under a CC-BY license. Not internally tested by R&D Systems.

Knockdown of IRE1 alpha reduces LPS-induced cytokine production.THP-1 cells transfected with either noncoding (NC) or IRE1 alpha targeting siRNA were treated with either 1 μg/ml LPS alone for 24 h or 1 μg/ml LPS for 24 h followed by addition of 10 μM nigericin (NG) for 45 min. a Cell lysates were analysed via immunoblotting for IRE1 alpha , phospho-p65, total p65, NLRP3, pro-caspase-1 and pro-IL-1 beta . Actin was used as a loading control. b Cell conditioned medium was analysed via immunoblotting for pro-IL-1 beta processing. c–f Levels of IL-1 beta , IL-8, TNF-alpha , and IL-6 were assayed in cell conditioned medium by ELISA (n = 3). *P < 0.05, **P < 0.01, and ***P < 0.001 based on a Student’s t test. Error bars represent SD Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31417078), licensed under a CC-BY license. Not internally tested by R&D Systems.

Oral live L. plantarum GKD7 reduced levels of TNF-alpha and IL-1 beta expression in OA synovium. (A) IHC staining of IL-1 beta and TNF-alpha in representative synovium from controls, ACLT-only rats, and ACLT + L. plantarum GKD7 rats. Quantitative analyses of (B) IL-1 beta and (C) TNF-alpha in synovium. Scale bar = 100 µm. * p < 0.05 vs. controls; # p < 0.05 vs. the ACLT-only group. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35956346), licensed under a CC-BY license. Not internally tested by R&D Systems.

Oral live L. plantarum GKD7 reduced key proinflammatory cytokines in OA cartilage. (A) IHC staining of IL-1 beta and TNF-alpha expression in representative cartilage from controls, ACLT-only rats, and ACLT + L. plantarum GKD7 rats. Quantitative analyses of (B) IL-1 beta and (C) TNF-alpha in cartilage. Scale bar = 100 µm. * p < 0.05 vs. controls; # p < 0.05 vs. the ACLT-only group. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35956346), licensed under a CC-BY license. Not internally tested by R&D Systems.

201-LB) was serially diluted and captured by Mouse Anti-Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB601) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

201-LB) was serially diluted and captured by Mouse Anti-Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB601) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB601U-100UG
Alexa Fluor 405	FAB601V-100UG
Alexa Fluor 488	FAB601G-100UG
Alexa Fluor 532	FAB601X-100UG
Alexa Fluor 594	FAB601T-100UG
Alexa Fluor 647	FAB601R-100UG
Alexa Fluor 700	FAB601N-100UG
Alexa Fluor 750	FAB601S-100UG
Alexa Fluor Plus 405	FAB601AFP405-100UG
Alexa Fluor Plus 488	FAB601AFP488-100UG
Alexa Fluor Plus 555	FAB601AFP555-100UG
Alexa Fluor Plus 594	FAB601AFP594-100UG
Alexa Fluor Plus 647	FAB601AFP647-100UG
Alexa Fluor Plus 680	FAB601AFP680-100UG
Allophycocyanin	FAB601A
Biotin	FAB601B
CoraFluor 1	FAB601CL1
DyLight 350	FAB601D
DyLight 405	FAB601E
DyLight 488	FAB601K
DyLight 550	FAB601L
DyLight 594	FAB601M
DyLight 650	FAB601W
DyLight 680	FAB601Y
DyLight 755	FAB601Z
FITC	FAB601F
HRP	FAB601H
Janelia Fluor 525	FAB601JF525
Janelia Fluor 549	FAB601I
Janelia Fluor 646	FAB601J
Janelia Fluor 669	FAB601JF669
PE	FAB601P
PerCP	FAB601C
mFluor Violet 450 SE	FAB601MFV450
mFluor Violet 500 SE	FAB601MFV500
mFluor Violet 610 SE	FAB601MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB601-SP
Carrier Free	MAB601-500

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(select up to 3 products from this page to compare)

AF-501-NA

IL-1 beta/IL-1F2 Antibody [Unconjugated]

(67 Publications)

100 μg

AF-501-NA

501-RL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-1 beta /IL-1F2 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-501-NA). The ND₅₀ is typically 2-10 µg/mL." class="big_lightbox">

Recombinant Rat IL-1 beta /IL-1F2 (Catalog # <a class=

501-RL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-1 beta /IL-1F2 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-501-NA). The ND₅₀ is typically 2-10 µg/mL." alt="Recombinant Rat IL-1 beta /IL-1F2 (Catalog # 501-RL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-1 beta /IL-1F2 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-501-NA). The ND₅₀ is typically 2-10 µg/mL." class="big_thumb" />

NL001) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

IL‑1 beta /IL‑1F2 was detected in immersion fixed LPS-stimulated rat splenocytes using 5 µg/mL Rat IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑501‑NA) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

NL001) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

Spatiotemporal analysis of retinal Il-1 beta protein levels following LD.A-G: Immunohistochemical assessment of Il-1 beta expression (green) in retinal cryosections over the course of LD. A: Immunoreactivity (IR) for Il-1 beta protein was not observed in dim-reared animals. B-D: Il-1 beta -IR was present among ramified nuclei situated within the ONL and OS (arrowheads) immediately following exposure to 24hrs LD. E-F: Il-1 beta -IR co-localised with IBA1+ cells (red) situated in the ONL/OS, though was not apparent in IBA1+ cells outside the vicinity of the ONL (asterisks). G-H: Il-1 beta -expressing cells (H, arrowheads) did not show any discernible IR for the M2 marker CD206 (red). I: Negative control sections, in which the primary antibody was omitted, did not show any resemblance to the IR for Il-1 beta evidenced in C-D at 24hrs LD. J: ELISA for Il-1 beta protein indicated an increased abundance of the protein immediately after 24hrs LD (P<0.05), and which was virtually undetectable at all other time points. C, choroid; GCL, ganglion cell layer; INL, inner nuclear layer; IHC, immunohistochemistry; ONL, outer nuclear layer; OS, outer segments; RPE, retinal pigment epithelium. The trend in ELISA protein levels was significant by ANOVA (P < 0.05); N = 3 for each timepoint. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26630454), licensed under a CC-BY license. Not internally tested by R&D Systems.

Spatiotemporal analysis of retinal CD206 protein levels following LD.A-L: investigation of CD206 immunoreactivity (IR, green) in retinal cryosections over the course of LD. A-B: In dim-reared animals, immunoreactivity (IR) for Il-1 beta protein was occasionally observed within nuclei (arrowheads) amongst the choroid (A) and ciliary body (B). C-E: Following 24hrs LD, CD206+ nuclei appeared from the ciliary body (C-D, arrowheads) and among the superficial retinal vasculature (E, arrowhead) F: At 24hrs LD, CD206+ cells were also more abundant within the ciliary body (arrowheads). G-I: There was increased abundance of CD206+ nuclei among optic nerve head (G-H) and superficial retinal vasculature (I) after 3 days post-exposure (arrowheads), compared to 24hrs LD. J: CD206+ cells were occasionally found accumulating within the choroid at 7 days post-exposure (arrowheads). K-L: All IR for CD206 was found to correlate with circular IBA1+ cells (red). M-N: CD206-expressing cells (N, arrowhead) did not show any detectable IR for the M1 marker Il-1 beta (red). O: Quantification of CD206 protein levels in retinas via ELISA. At 3 and 7 days post-exposure, the levels of CD206 protein were significantly higher compared to dim-reared controls (P<0.05). Progressive increases were observed during the post-exposure period, though this was not significant between 3 and 7 days (P>0.05). C, choroid; GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; IHC, immunohistochemistry; ONL, outer nuclear layer; OS, outer segments; RPE, retinal pigment epithelium. The trend in ELISA protein levels was significant by ANOVA (P < 0.05); N = 3 for each timepoint. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26630454), licensed under a CC-BY license. Not internally tested by R&D Systems.

Correlation of CD206 and Il-1 beta immunolabelling within the CD11b+ macrophage population following LD.A: Representative flow cytometry plots examine CD206+ and Il1b+ cell counts within the CD11b population following light damage. For the most part, Il-1 beta and CD206 cells occupied mutually distinct subsets within the population CD11b cells. B: Quantification of Il-1 beta +/CD206- and CD206+/Il-1 beta - cells as percentage of the CD11b+ population following LD. There was a sharp increase in the proportion of Il-1 beta +/CD206- cells immediately following 24hrs LD (P<0.05), though this then decreased dramatically afterward and was similar to control samples by 7 days (P>0.05). For CD206+/Il-1 beta - cells, there was no change in their proportion at 24hrs LD (P>0.05). At 3 days post-exposure however the proportion of CD206+/Il-1 beta - cells had tripled (P<0.05), though this was then reduced to near control proportions by 7 days post-exposure (P<0.05). The trend of both Il-1 beta +/CD206- and CD206+/Il-1 beta - cells across the time course were significant by ANOVA (P < 0.05); N = 5 for each timepoint. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26630454), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>The effect of chronic clomipramine treatment on the hippocampal NLRP3 inflammasome level in the CMS-treated rats.The protein levels of A IL-1 beta , B cleaved caspase-1, C NLRP3, D ASC, and E pro-caspase-1 (n = 4/group) were analyzed by western blot. All data are expressed as the mean ± SD. ##p < 0.01, ###p < 0.001 compared to saline-treated rats. *p < 0.05, **p <0.01, ***p < 0.001 compared to non-stressed control rats. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35688836), licensed under a CC-BY license. Not internally tested by R&D Systems.

Sustained IL-1 beta up-regulation in hippocampal astrocytes.IL-1 beta immunoreactivity was measured in GFAP-positive cells after LPS exposure or saline. Levels of IL-1 beta were found up-regulated from day 1 up to day 30. Higher magnification insets highlight the co-localization of IL-1 beta with GFAP (A). The ratio of IL-1 beta positive astrocytes in total astrocytes (GFAP positive cells) was quantified (B). Pictures show DG area, data are expressed as mean ± standard error of the mean (n = 4) and compared by 1-way analysis of variance followed with Boferroni post hoc analysis, **p<0.01, ***p<0.001 vs Control. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0106331), licensed under a CC-BY license. Not internally tested by R&D Systems.

501-RL) was serially diluted and captured by Goat Anti-Rat IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-501-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-501-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

501-RL) was serially diluted and captured by Goat Anti-Rat IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-501-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-501-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

501-RL) was serially diluted and captured by Goat Anti-Rat IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-501-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-501-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

501-RL) was serially diluted and captured by Goat Anti-Rat IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-501-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-501-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF-501-NAU-100UG
Alexa Fluor 405	AF-501-NAV-100UG
Alexa Fluor 488	AF-501-NAG-100UG
Alexa Fluor 532	AF-501-NAX-100UG
Alexa Fluor 594	AF-501-NAT-100UG
Alexa Fluor 647	AF-501-NAR-100UG
Alexa Fluor 700	AF-501-NAN-100UG
Alexa Fluor 750	AF-501-NAS-100UG
Alexa Fluor Plus 405	AF-501-NAAFP405
Alexa Fluor Plus 488	AF-501-NAAFP488
Alexa Fluor Plus 555	AF-501-NAAFP555
Alexa Fluor Plus 594	AF-501-NAAFP594
Alexa Fluor Plus 647	AF-501-NAAFP647
Alexa Fluor Plus 680	AF-501-NAAFP680
Allophycocyanin	AF-501-NAA
Biotin	BAF501
CoraFluor 1	AF-501-NACL1
DyLight 350	AF-501-NAD
DyLight 405	AF-501-NAE
DyLight 488	AF-501-NAK
DyLight 550	AF-501-NAL
DyLight 594	AF-501-NAM
DyLight 650	AF-501-NAW
DyLight 680	AF-501-NAY
DyLight 755	AF-501-NAZ
FITC	AF-501-NAF
HRP	AF-501-NAH
Janelia Fluor 525	AF-501-NAJF525
Janelia Fluor 549	AF-501-NAI
Janelia Fluor 646	AF-501-NAJ
Janelia Fluor 669	AF-501-NAJF669
PE	AF-501-NAP
PerCP	AF-501-NAC
mFluor Violet 450 SE	AF-501-NAMFV450
mFluor Violet 500 SE	AF-501-NAMFV500
mFluor Violet 610 SE	AF-501-NAMFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF-501-SP

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501-RL

Recombinant Rat IL-1 beta/IL-1F2 Protein

(65 Publications)

10 μg

501-RL

1 μg/lane of Recombinant Rat IL-1 beta /IL-1F2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 18 kDa.

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

Applications:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	501-RL-010/CF
Carrier Free	501-RL-050/CF

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MAB401

IL-1 beta/IL-1F2 Antibody (30311) [Unconjugated]

(57 Publications)

100 μg

MAB401

401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1 beta /IL-1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB401). The ND₅₀ is typically 1-5 µg/mL." class="big_lightbox">

Recombinant Mouse IL-1 beta /IL-1F2 (Catalog # <a class=

401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1 beta /IL-1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB401). The ND₅₀ is typically 1-5 µg/mL." alt="Recombinant Mouse IL-1 beta /IL-1F2 (Catalog # 401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1 beta /IL-1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB401). The ND₅₀ is typically 1-5 µg/mL." class="big_thumb" />

401-ML) was serially diluted and captured by Rat Anti-Mouse IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB401) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

401-ML) was serially diluted and captured by Rat Anti-Mouse IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB401) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

401-ML) was serially diluted and captured by Rat Anti-Mouse IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB401) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

401-ML) was serially diluted and captured by Rat Anti-Mouse IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB401) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

401-ML) was serially diluted and captured by Rat Anti-Mouse IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB401) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

401-ML) was serially diluted and captured by Rat Anti-Mouse IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB401) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

401-ML) was serially diluted and captured by Rat Anti-Mouse IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB401) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

401-ML) was serially diluted and captured by Rat Anti-Mouse IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB401) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

401-ML) was serially diluted and captured by Rat Anti-Mouse IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB401) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

401-ML) was serially diluted and captured by Rat Anti-Mouse IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB401) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB401U-100UG
Alexa Fluor 405	FAB401V-100UG
Alexa Fluor 488	FAB401G-100UG
Alexa Fluor 532	FAB401X-100UG
Alexa Fluor 594	FAB401T-100UG
Alexa Fluor 647	FAB401R-100UG
Alexa Fluor 700	FAB401N-100UG
Alexa Fluor 750	FAB401S-100UG
Alexa Fluor Plus 405	FAB401AFP405-100UG
Alexa Fluor Plus 488	FAB401AFP488-100UG
Alexa Fluor Plus 555	FAB401AFP555-100UG
Alexa Fluor Plus 594	FAB401AFP594-100UG
Alexa Fluor Plus 647	FAB401AFP647-100UG
Alexa Fluor Plus 680	FAB401AFP680-100UG
Allophycocyanin	FAB401A
CoraFluor 1	FAB401CL1
DyLight 350	FAB401D
DyLight 405	FAB401E
DyLight 488	FAB401K
DyLight 550	FAB401L
DyLight 594	FAB401M
DyLight 650	FAB401W
DyLight 680	FAB401Y
DyLight 755	FAB401Z
FITC	FAB401F
HRP	FAB401H
Janelia Fluor 525	FAB401JF525
Janelia Fluor 549	FAB401I
Janelia Fluor 646	FAB401J
Janelia Fluor 669	FAB401JF669
PE	FAB401P
PerCP	FAB401C
mFluor Violet 450 SE	FAB401MFV450
mFluor Violet 500 SE	FAB401MFV500
mFluor Violet 610 SE	FAB401MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB401-SP
Carrier Free	MAB401-500

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AB-401-NA

IL-1 beta/IL-1F2 Antibody [Unconjugated]

(33 Publications)

1 mg

AB-401-NA

401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1 beta /IL-1F2 (10 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1 beta /IL-1F2 Polyclonal Antibody (Catalog # AB-401-NA). The ND₅₀ is typically 2-12 µg/mL." class="big_lightbox">

Recombinant Mouse IL-1 beta /IL-1F2 (<a class=

401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1 beta /IL-1F2 (10 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1 beta /IL-1F2 Polyclonal Antibody (Catalog # AB-401-NA). The ND₅₀ is typically 2-12 µg/mL." alt="Recombinant Mouse IL-1 beta /IL-1F2 (401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1 beta /IL-1F2 (10 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1 beta /IL-1F2 Polyclonal Antibody (Catalog # AB-401-NA). The ND₅₀ is typically 2-12 µg/mL." class="big_thumb" />

HAF017). A specific band was detected for IL-1 beta /IL-1F2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

HAF017). A specific band was detected for IL-1 beta /IL-1F2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

<b>Biological Strategies Validation. </b>Simple Western lane view shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 24 hours, loaded at 0.5 mg/mL. A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 40 kDa (as indicated) using 50 µg/mL of Goat Anti-Mouse IL‑1 beta /IL‑1F2 Polyclonal Antibody (Catalog # AB-401-NA) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

GlcN inhibits caspase-1 activation and the release of IL-1 beta , IL-18 and ASC. (A–D) J774A.1 macrophages were incubated for 4 h with LPS (1 µg/ml) followed by incubation for 2 h with GlcN. Cells were then incubated with ATP (5 mM, 0.5 h) or infected with E. coli (30 MOI, 1 h). The expression levels of IL-1 beta and IL-18 (A), caspase-1 (C), ASC (D) in the supernatants and IL-1 beta in the cell lysates (B) were analysed by Western blotting. (E) J774A.1 macrophages were incubated for 4 h with LPS (1 µg/ml) followed by incubation for 2 h with GlcN, D-(+)-glucose (Glu), D-glucosamine 3-sulphate (GlcN-3S), D-(+)-galactosamine hydrochloride (GalN) and N-acetyl-D-glucosamine (GlcNAc) for 2 h, followed by incubation with ATP (5 mM) for 0.5 h. The IL-1 beta expression levels in the supernatants were measured by ELISA. (F) J774A.1 macrophages were incubated for 4 h with LPS (1 µg/ml) or PamsCSK4 (1 µg/ml; for non-canonical inflammasome) followed by incubation for 2 h with GlcN, followed by transfection with poly(dA/dT) (2 µg/ml) or LPS (2 µg/ml) for 6 h or by Salmonella infection (30 MOI) for 2 h. The IL-1 beta expression levels in the supernatants were measured by ELISA. The ELISA data are expressed as the mean ± SD of separate experiments as indicated. The Western blotting results are representative of three different experiments and the histogram shows the quantification expressed as the mean ± SD for these three experiments. *, *** and **** indicate a significant difference at the level of p < 0.05, p < 0.001 and p < 0.0001, respectively, compared to NLRP3 activator-treated cells. (One-way ANOVA with Dunnett’s multiple comparisons test). The blots in (A–D) were cropped from different gels; full-length blots are included in the “Supplementary Information”. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30944389), licensed under a CC-BY license. Not internally tested by R&D Systems.

CVL reduced NLRP3 inflammasome activation. (A) J774A.1 macrophages were incubated for 5 h with LPS (1 μg/ml) (LPS priming) followed by incubation for 0.5 h with CVL. Cells were then incubated with CC (100 μg/ml, 24 h), MSU (100 μg/ml, 24 h), ATP (5 mM, 0.5 h), nigericin (10 μM, 0.5 h), and nano-SiO2 (100 μg/ml, 24 h). (B) LPS-primed J774A.1 macrophages were incubated for 0.5 h with CVL followed by incubation with CC (100 μg/ml, 24 h), MSU (100 μg/ml, 24 h), and ATP (5 mM, 0.5 h). (C) LPS-primed BMDM were incubated for 0.5 h with CVL followed by incubation with CC (100 μg/ml) for an additional 24 h. (D) LPS-primed or Pam3CSK4-primed (for LPS transfection only) cells were incubated for 0.5 h with CVL followed by transfection with poly(dA/dT) (2 μg/ml, 6 h), FLA-ST (1 μg/ml, 6 h), MDP (10 μg/ml, 6 h), or LPS (2 μg/ml, 6 h). The levels of IL-1 beta , IL-18, NLRP3, ASC, and caspase-1 in the culture medium were measured by Western blot. The IL-1 beta levels in the supernatants were measured by ELISA. The Western blot results are representative of three different experiments. The ELISA data are expressed as the mean ± SD of three separate experiments. *, **, and *** indicate a significant difference at the level of p < 0.05, p < 0.01, and p < 0.001, respectively, compared to activator-treated cells. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30186288), licensed under a CC-BY license. Not internally tested by R&D Systems.

F240B inhibits the NLRP3 inflammasome through autophagy induction. (A, B) J774A.1 macrophages were incubated with 1 µg/ml LPS for 5 h followed by incubated with F240B for 3 h. Cells then incubated with 5 mM ATP for 0.5 h. The levels of IL-1 beta and caspase-1 (A) or NLRP3 and ASC (B) in the supernatants were measured by Western blotting. (C) J774A.1 macrophages were incubated with 1 µg/ml LPS for 5 h followed by incubated with 1 µM F240B in the presence or absence of 5 mM 3-MA for 3 h. Cells then were incubated with 5 mM ATP or 10 μM nigericin for 0.5 h. The levels of IL-1 beta in the supernatants were measured by ELISA. (D) Wild-type and LC3-knockout J774A.1 macrophages were incubated with 1 µg/ml LPS for 5 h followed by incubated with 1 µM F240B for 3 h. Cells then incubated with 5 mM ATP or 10 μM nigericin for 0.5 h. The levels of IL-1 beta in the supernatants were measured by ELISA. The data are expressed as the mean ± SD of three separate experiments. * and *** indicate a significant difference at the level of p < 0.05 and p < 0.001, respectively. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33424855), licensed under a CC-BY license. Not internally tested by R&D Systems.

ALKBH5 regulates the expression of innate immune response genes during bacterial and viral infections in RAW264.7 cells. (A) ALKBH5 knockdown enhanced the expression levels of the IL-1 beta , CSF3, TGM2, and SRC transcripts during P. aeruginosa, C. diphtheriae, HSV-1, or HSV-1 ICP34.5 mutant infection. (B to D) ALKBH5 knockdown enhanced the protein level of IL-1 beta during infection by C. diphtheriae (B), P. aeruginosa (C), or the HSV-1 ICP34.5 mutant (D). (E) ALKBH5 overexpression inhibited the expression of the IL-1 beta , CSF3, TGM2, and SRC genes during P. aeruginosa infection as measured by RT-qPCR. (F) ALKBH5 overexpression inhibited the protein level of IL-1 beta during P. aeruginosa infection as measured by Western blotting. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36625590), licensed under a CC-BY license. Not internally tested by R&D Systems.

DUSP1 regulates p38 and JNK phosphorylation and the expression of innate immune response genes during bacterial and viral infections in RAW264.7 cells. (A) DUSP1 knockdown enhanced p38 and JNK phosphorylation during infection by P. aeruginosa, C. diphtheriae, HSV-1, or the HSV-1 ICP34.5 mutant. (B) DUSP1 knockdown enhanced the expression of the IL-1 beta , CSF3, TGM2, and SRC genes during infection by P. aeruginosa, C. diphtheriae, HSV-1, or the HSV-1 ICP34.5 mutant. (C) DUSP1 knockdown enhanced the protein level of IL-1 beta during infection by P. aeruginosa, C. diphtheriae, or the HSV-1 ICP34.5 mutant. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36625590), licensed under a CC-BY license. Not internally tested by R&D Systems.

CS inhibits NLRP3 inflammasome activation. (A–D) J774A.1 macrophages were primed with LPS for 5 h and incubated with CS for 0.5 h Cells were stimulated with 10 μM nigericin for 0.5 h The levels of IL-1 beta in the supernatants were measured by ELISA (A) and Western blot (B). The levels of IL-18 (C) and caspase-1 (D) in the supernatants were measured by Western blot. (E, F) Human THP-1 macrophages or PBMC were primed with LPS for 5 h and incubated with CS for 0.5 h Cells were stimulated with 10 μM nigericin for 0.5 h The levels of IL-1 beta in the supernatants were measured by ELISA. (G, H) J774A.1 macrophages were primed with LPS for 5 h and incubated with CS for 0.5 h Cells were stimulated with 5 mM ATP for 0.5 h The levels of IL-1 beta in the supernatants were measured by ELISA (G) and the levels of caspase-1 in the supernatants were measured by Western blot (H). (I) J774A.1 macrophages were primed with LPS or Pam3CSK4 (for non-canonical inflammasome) for 5 h and incubated with CS for 0.5 h Cells were transfected with poly(dA/dT), LPS, MDP or FLA-ST for 6 h or infected with Salmonella for 2 h The levels of IL-1 beta in the supernatants were measured by ELISA. (J) J774A.1 macrophages were primed with LPS for 5 h and incubated with Irbesartan (IS) for 0.5 h Cells were stimulated with 10 μM nigericin for 0.5 h The levels of IL-1 beta in the supernatants were measured by ELISA. The ELISA data are expressed as the means ± SD of the three separate experiments. The Western blot images are representative results, and the histogram shows the band intensity. *, ** and *** indicate a significant difference at the level of p<0.05, p<0.01 and p<0.001, respectively, compared to nigericin- or ATP-activated cells or as indicated. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35669789), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-19775

IL-1 beta/IL-1F2 Antibody - BSA Free

(21 Publications)

0.1 mg

NBP1-19775

Immunohistochemistry: IL-1 beta/IL-1F2 Antibody [NBP1-19775] - Staining in the cytoplasm of villi and in the crypt cells of human lung carcinoma tissue.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-19775AF350
Alexa Fluor 405	NBP1-19775AF405
Alexa Fluor 488	NBP1-19775AF488
Alexa Fluor 532	NBP1-19775AF532
Alexa Fluor 594	NBP1-19775AF594
Alexa Fluor 647	NBP1-19775AF647
Alexa Fluor 700	NBP1-19775AF700
Alexa Fluor 750	NBP1-19775AF750
Alexa Fluor Plus 405	NBP1-19775AFP405
Alexa Fluor Plus 488	NBP1-19775AFP488
Alexa Fluor Plus 555	NBP1-19775AFP555
Alexa Fluor Plus 594	NBP1-19775AFP594
Alexa Fluor Plus 647	NBP1-19775AFP647
Alexa Fluor Plus 680	NBP1-19775AFP680
Allophycocyanin	NBP1-19775APC
Biotin	NBP1-19775B
CoraFluor 1	NBP1-19775CL1
DyLight 350	NBP1-19775UV
DyLight 405	NBP1-19775V
DyLight 488	NBP1-19775G
DyLight 550	NBP1-19775R
DyLight 594	NBP1-19775DL594
DyLight 650	NBP1-19775C
DyLight 680	NBP1-19775FR
DyLight 755	NBP1-19775IR
FITC	NBP1-19775F
HRP	NBP1-19775H
Janelia Fluor 525	NBP1-19775JF525
Janelia Fluor 549	NBP1-19775JF549
Janelia Fluor 646	NBP1-19775JF646
Janelia Fluor 669	NBP1-19775JF669
PE	NBP1-19775PE
PerCP	NBP1-19775PCP
mFluor Violet 450 SE	NBP1-19775MFV450
mFluor Violet 500 SE	NBP1-19775MFV500
mFluor Violet 610 SE	NBP1-19775MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-19775SS

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NBP1-42767

IL-1 beta/IL-1F2 Antibody - Azide and BSA Free

(1 Review)

(12 Publications)

0.1 mg

NBP1-42767

Immunohistochemistry: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Analysis of Mouse Embryonic Kidney Tissue: Mouse Embryonic Kidney Fixation: FFPE buffered formalin 10% conc Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker Primary antibody: 2ug/ml 1.5 hour @ room T Secondary Ab: MACH 1 HRP POLYMER 1:50 45C RT

Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Analysis using the Biotin conjugate of NBP1-42767. Detection of Lane 1: Mouse IL-1beta Recombinant Protein. Load: 50 ng per lane. Used at a dilution of 1:1,000 overnight at 4C.

Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Detects 10% of the non-denatured (native) precursor 31,000 MW mouse IL-1Beta containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in western blot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1Beta precursor molecule. Denatured IL-1Beta will have a 18 kDa band.

Immunohistochemistry of Rabbit anti-IL-1 beta/IL-1F2 Antibody in Mouse Embryonic Kidney Tissue: Mouse Embryonic Kidney Fixation: FFPE buffered formalin 10% conc Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker Primary antibody: 2ug/ml 1.5 hour @ room T Secondary Ab: MACH 1 HRP POLYMER 1:50 45

This antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW mouse IL-1beta containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in western blot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1beta precursor molecule. Denatured IL-1beta will have a 18 kDa band.

HIV Vpu and gp41 play a role in inflammasome activation.Monocyte derived macrophages (MDMs) (1 x 106) were either left untreated or transfected with 2μg of pVpu plasmid expressing Vpu or pgp41 or gp120 plasmid expressing gp120. Supernatants were collected and tested for IL-1 beta and IL-18 secretion at 12 hr (A) and 24 hr (B). Cells extracts at 12 hr from mock or transfected cells were analysed for the presence of caspase-1 p20 by western blotting, followed by quantification using Image Studio Lite (Licor) and normalized to internal control ( beta -actin) from 3 western blots(C). MDMs expressing gp41 (D) or Vpu (E) monocytes were silenced for TLR2, TLR3, TLR4 and TLR8 by siRNA. Supernatant was collected at 12 hr and analysed for IL-1 beta and IL-18 using ELISA. Cell extracts of MDMs stimulated with either Vpu (F) or gp41 (G) were analysed for the presence of pro-IL-1 beta , pro-IL-18, cleaved IL-1 beta or cleave IL-18 by western blotting, followed by quantification using Image Studio Lite (Licor) and normalized to internal control ( beta -actin) from 3 western blots. MDMs expressing Vpu (H-J), gp41 (K-M) or pgmock (N-P) were silenced for NLRP3, NLRP1, NLRC4 or NLRP3 and NLRC4 by siRNA. Supernatant was collected at 12 hr and analysed for IL-1 beta (Η, Κ, Ν) and IL-18 (I,L,O) using ELISA. All graphs were normalized to the level of siRNA knockdown. Cells extracts were analysed for the presence of caspase-1 p20 at 12 hr by western blotting, followed by quantification using Image Studio Lite (Licor) and normalized to internal control ( beta -actin) from 3 western blots (J,M,P). The data represent the mean of three independent experiments +/- SD (n = 3 sets of macrophages) yielding consistent results. **, p < 0.005 and ***, p < 0.001 indicate statistically significant differences. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33861800), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Mu, Rt

Species Glossary

Reactivity: Mu, Rt

Applications:

, , , , , , ,

Applications: , , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-42767AF350
Alexa Fluor 405	NBP1-42767AF405
Alexa Fluor 488	NBP1-42767AF488
Alexa Fluor 532	NBP1-42767AF532
Alexa Fluor 594	NBP1-42767AF594
Alexa Fluor 647	NBP1-42767AF647
Alexa Fluor 700	NBP1-42767AF700
Alexa Fluor 750	NBP1-42767AF750
Alexa Fluor Plus 405	NBP1-42767AFP405
Alexa Fluor Plus 488	NBP1-42767AFP488
Alexa Fluor Plus 555	NBP1-42767AFP555
Alexa Fluor Plus 594	NBP1-42767AFP594
Alexa Fluor Plus 647	NBP1-42767AFP647
Alexa Fluor Plus 680	NBP1-42767AFP680
Allophycocyanin	NBP1-42767APC
Biotin	NBP1-42768
Biotin	NBP1-42767B
CoraFluor 1	NBP1-42767CL1
DyLight 350	NBP1-42767UV
DyLight 405	NBP1-42767V
DyLight 488	NBP1-42767G
DyLight 550	NBP1-42767R
DyLight 594	NBP1-42767DL594
DyLight 650	NBP1-42767C
DyLight 680	NBP1-42767FR
DyLight 755	NBP1-42767IR
FITC	NBP1-42767F
HRP	NBP1-42769
HRP	NBP1-42767H
Janelia Fluor 525	NBP1-42767JF525
Janelia Fluor 549	NBP1-42767JF549
Janelia Fluor 646	NBP1-42767JF646
Janelia Fluor 669	NBP1-42767JF669
PE	NBP1-42767PE
PerCP	NBP1-42767PCP
mFluor Violet 450 SE	NBP1-42767MFV450
mFluor Violet 500 SE	NBP1-42767MFV500
mFluor Violet 610 SE	NBP1-42767MFV610

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DY681

Porcine IL-1 beta/IL-1F2 DuoSet ELISA, 15 Plate

(18 Publications)

1 Kit (for 15 Plates)

DY681

Reactivity:

Po

Species Glossary

Reactivity: Po

Applications:

Applications:

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MAB4012

IL-1 beta/IL-1F2 Antibody (B122) [Unconjugated]

(14 Publications)

100 μg

MAB4012

401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1 beta /IL-1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Mouse IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB4012). The ND₅₀ is typically 0.3-1.5 µg/mL." class="big_lightbox">

401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1 beta /IL-1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Mouse IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB4012). The ND₅₀ is typically 0.3-1.5 µg/mL." alt="Recombinant Mouse IL-1 beta /IL-1F2 (Catalog # 401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1 beta /IL-1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Mouse IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB4012). The ND₅₀ is typically 0.3-1.5 µg/mL." class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Hamster Monoclonal

Host: Hamster Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB4012U-100UG
Alexa Fluor 405	FAB4012V-100UG
Alexa Fluor 488	FAB4012G-100UG
Alexa Fluor 532	FAB4012X-100UG
Alexa Fluor 594	FAB4012T-100UG
Alexa Fluor 647	FAB4012R-100UG
Alexa Fluor 700	FAB4012N-100UG
Alexa Fluor 750	FAB4012S-100UG
Alexa Fluor Plus 405	FAB4012AFP405-100UG
Alexa Fluor Plus 488	FAB4012AFP488-100UG
Alexa Fluor Plus 555	FAB4012AFP555-100UG
Alexa Fluor Plus 594	FAB4012AFP594-100UG
Alexa Fluor Plus 647	FAB4012AFP647-100UG
Alexa Fluor Plus 680	FAB4012AFP680-100UG
Allophycocyanin	FAB4012A
Biotin	FAB4012B
CoraFluor 1	FAB4012CL1
DyLight 350	FAB4012D
DyLight 405	FAB4012E
DyLight 488	FAB4012K
DyLight 550	FAB4012L
DyLight 594	FAB4012M
DyLight 650	FAB4012W
DyLight 680	FAB4012Y
DyLight 755	FAB4012Z
FITC	FAB4012F
HRP	FAB4012H
Janelia Fluor 525	FAB4012JF525
Janelia Fluor 549	FAB4012I
Janelia Fluor 646	FAB4012J
Janelia Fluor 669	FAB4012JF669
PE	FAB4012P
PerCP	FAB4012C
mFluor Violet 450 SE	FAB4012MFV450
mFluor Violet 500 SE	FAB4012MFV500
mFluor Violet 610 SE	FAB4012MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB4012-SP

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MAB4011

IL-1 beta/IL-1F2 Antibody (166926) [Unconjugated]

(12 Publications)

100 μg

MAB4011

HAF005). A specific band was detected for IL-1 beta /IL-1F2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with LPS. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB4011) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # <a class=

HAF005). A specific band was detected for IL-1 beta /IL-1F2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with LPS. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB4011) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-1 beta /IL-1F2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

<b>Biological Strategies Validation. </b>Pleural cells are involved in bleomycin-induced inflammation. a Cell recruitment in PLF was assessed by total cell count at D3, D14 and D21. Results are expressed as mean ± SEM. b Inflammation was estimated by differential count in PLF after May-Grünwald Giemsa staining. Results are expressed as percentages of total cells. n = 4 for NaCl groups, n = 6 for BLM groups at D3 and D21, n = 6 for NaCl and n = 8 for BLM at D14. c, d Caspase-1 activation was assessed at D14 by c western blot in whole lung extracts (n = 3 mice/group) or by d specific caspase-1 FLICA analysis on lung section from NaCl or BLM injected mice (Representative images are shown. Active caspase-1: green, DAPI: blue, dotted lines represent the pleura, scale bars: 50 μm). e, f Met5A cells were treated with BLM (100 μM) and e activation of caspase-1 was followed (western blot; left, densitometric analysis; right). f active IL-1 beta was assessed in the supernatant of Met5A cells after 24 h of culture. Representative results from 3 independent experiments. HSC70 serves as a loading control. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 Image collected and cropped by CiteAb from the following publication (//respiratory-research.biomedcentral.com/articles/10.1186/s12931-016-0475-8), licensed under a CC-BY license. Not internally tested by R&D Systems.

Pleural cells are involved in bleomycin-induced inflammation. a Cell recruitment in PLF was assessed by total cell count at D3, D14 and D21. Results are expressed as mean ± SEM. b Inflammation was estimated by differential count in PLF after May-Grünwald Giemsa staining. Results are expressed as percentages of total cells. n = 4 for NaCl groups, n = 6 for BLM groups at D3 and D21, n = 6 for NaCl and n = 8 for BLM at D14. c, d Caspase-1 activation was assessed at D14 by c western blot in whole lung extracts (n = 3 mice/group) or by d specific caspase-1 FLICA analysis on lung section from NaCl or BLM injected mice (Representative images are shown. Active caspase-1: green, DAPI: blue, dotted lines represent the pleura, scale bars: 50 μm). e, f Met5A cells were treated with BLM (100 μM) and e activation of caspase-1 was followed (western blot; left, densitometric analysis; right). f active IL-1 beta was assessed in the supernatant of Met5A cells after 24 h of culture. Representative results from 3 independent experiments. HSC70 serves as a loading control. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/27894300), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB4011U-100UG
Alexa Fluor 405	FAB4011V-100UG
Alexa Fluor 488	FAB4011G-100UG
Alexa Fluor 532	FAB4011X-100UG
Alexa Fluor 594	FAB4011T-100UG
Alexa Fluor 647	FAB4011R-100UG
Alexa Fluor 700	FAB4011N-100UG
Alexa Fluor 750	FAB4011S-100UG
Alexa Fluor Plus 405	FAB4011AFP405-100UG
Alexa Fluor Plus 488	FAB4011AFP488-100UG
Alexa Fluor Plus 555	FAB4011AFP555-100UG
Alexa Fluor Plus 594	FAB4011AFP594-100UG
Alexa Fluor Plus 647	FAB4011AFP647-100UG
Alexa Fluor Plus 680	FAB4011AFP680-100UG
Allophycocyanin	FAB4011A
Biotin	FAB4011B
CoraFluor 1	FAB4011CL1
DyLight 350	FAB4011D
DyLight 405	FAB4011E
DyLight 488	FAB4011K
DyLight 550	FAB4011L
DyLight 594	FAB4011M
DyLight 650	FAB4011W
DyLight 680	FAB4011Y
DyLight 755	FAB4011Z
FITC	FAB4011F
HRP	FAB4011H
Janelia Fluor 525	FAB4011JF525
Janelia Fluor 549	FAB4011I
Janelia Fluor 646	FAB4011J
Janelia Fluor 669	FAB4011JF669
PE	FAB4011P
PerCP	FAB4011C
mFluor Violet 450 SE	FAB4011MFV450
mFluor Violet 500 SE	FAB4011MFV500
mFluor Violet 610 SE	FAB4011MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB4011-SP
Carrier Free	MAB4011-500

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MAB501

IL-1 beta/IL-1F2 Antibody (38139) [Unconjugated]

(8 Publications)

500 μg

MAB501

501-RL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-1 beta /IL-1F2 (4 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB501). The ND₅₀ is typically 10-30 µg/mL." class="big_lightbox">

501-RL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-1 beta /IL-1F2 (4 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB501). The ND₅₀ is typically 10-30 µg/mL." alt="Recombinant Rat IL-1 beta /IL-1F2 (Catalog # 501-RL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-1 beta /IL-1F2 (4 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB501). The ND₅₀ is typically 10-30 µg/mL." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

IL‑1 beta /IL‑1F2 was detected in Immersion fixed Rat Splenocytes cells using Mouse Anti-Rat IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB501) at 10 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

IL‑1 beta /IL‑1F2 was detected in perfusion fixed paraffin-embedded sections of rat intestine using Mouse Anti-Rat IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB501) at 15 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # <a class=

VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

IL‑1 beta /IL‑1F2 was detected in perfusion fixed paraffin-embedded sections of rat liver using Mouse Anti-Rat IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB501) at 15 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # <a class=

VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

IL‑1 beta /IL‑1F2 was detected in perfusion fixed paraffin-embedded sections of rat stomach using Mouse Anti-Rat IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB501) at 15 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # <a class=

VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB501U-100UG
Alexa Fluor 405	FAB501V-100UG
Alexa Fluor 488	FAB501G-100UG
Alexa Fluor 532	FAB501X-100UG
Alexa Fluor 594	FAB501T-100UG
Alexa Fluor 647	FAB501R-100UG
Alexa Fluor 700	FAB501N-100UG
Alexa Fluor 750	FAB501S-100UG
Alexa Fluor Plus 405	FAB501AFP405-100UG
Alexa Fluor Plus 488	FAB501AFP488-100UG
Alexa Fluor Plus 555	FAB501AFP555-100UG
Alexa Fluor Plus 594	FAB501AFP594-100UG
Alexa Fluor Plus 647	FAB501AFP647-100UG
Alexa Fluor Plus 680	FAB501AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB501-SP

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

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