VEGFR1/Flt-1: Novus Biologicals

AF321

VEGFR1/Flt-1 Antibody [Unconjugated]

(2 Reviews)

100 μg

AF321

Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

VEGFR1/Flt‑1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Human VEGFR1/Flt‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF321) overnight at 4 °C. Tissue was stained (red) and counterstained with hematoxylin (blue). View our protocol for <a class=

Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="VEGFR1/Flt‑1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Human VEGFR1/Flt‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF321) overnight at 4 °C. Tissue was stained (red) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

VEGFR1/Flt-1 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Human VEGFR1/Flt-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF321) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Exogenous VEGF165 suppresses RASSF1A expression in normal epithelial and endothelial cells. Metastatic colon cancer cells (mCRC, (A) T84 and (B) Colo 205) were stimulated with VEGF165 as indicated before and cell metabolism was performed by MTT assay for 6 h (black dash), 24 h (green) and 48 h (pink). Immortalized benign protate ((C) RWPE1), human bronchial epithelial cells ((D) HBEpc), immortalized human breast epithelial cells ((E) MCF-10A), endothelial cells (F, EC) were stimulated with or without VEGF165 (12.5 and 25 ng/mL) for 24 h. Cell lysates were monitored by Western blot for p-VEGFR1, p-VEGFR2, RASSF1A, and GAPDH as indicated. Immunoblot was quantified by scanning densitometry and normalized against GAPDH expression for RWPE1 (G), HBEpc (H), MCF 10A (I) and EC (lower panel of (F)). Results are from three independent experiments and statistical significance was determined using one way-ANOVA followed Bonferroni test. (* p < 0.05, ** p < 0.01, *** p < 0.001). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30744076), licensed under a CC-BY license. Not internally tested by R&D Systems.

AB-108-C, open histogram) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox">

HUVEC cells were stained with Goat Anti-Human VEGFR1/Flt-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF321, filled histogram) or isotype control antibody (Catalog # <a class=

AB-108-C, open histogram) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

VEGFR1 isoforms in human degenerated and bovine healthy discs. A representative immunoblotting assay showed the expression of the full-length membrane form (~ 200 kDa), the soluble form (~ 130 kDa) and a cytoplasmic fragment (~ 60 kDa) in human degenerated (a) and healthy bovine (c) disc cells. beta -Actin was used as control. Western blot quantification of membrane and soluble VEGFR1 was performed on human (b; n = 3) and bovine (d; n = 2) disc cells. Results represent an average of area (square pixels) ± SD normalized on beta Actin. White bars represent AF, grey bars NP. No statistical significance with non-parametric Mann–Whitney–Wilcoxon U test for independent variables Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29784013), licensed under a CC-BY license. Not internally tested by R&D Systems.

Decreased levels of VEGFR-2 in hypoxia and in early first trimester placentae.A: Representative VEGFR-2 and sFlt-1 immunoblots of placental villous explants cultured at 3% and 20% O2. Densitometry analysis comparing normalized VEGFR-2 immunoreactivity between explants cultured at 3% (n = 7) and 20% O2 (n = 7). VEGFR-2 transcript levels were assessed by qPCR analysis comparing 3% (n = 4) and 20% O2 (n = 4) samples. B: Representative VEGFR-2 immunoblots of early (EFT) and late (LFT) first trimester placentae. Densitometry analysis comparing normalized VEGFR-2 immunoreactivity between samples were not significantly different. Transcript levels were assessed by qPCR analysis comparing VEGFR-2 levels in EFT and LFT placentae. (EFT, n = 12; LFT, n = 14; *P<0.05). All values are represented as the means±SEM. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24260556), licensed under a CC-BY license. Not internally tested by R&D Systems.

sFlt-1 directly attenuates VEGFR-2 expression and downstream signalling in placental villous explants.A: Representative VEGFR-2, sFlt-1, pAkt and pERK immunoblots of first trimester placental villous explants treated with sFlt-1 or a blocking antibody to sFlt-1. Densitometry analyses comparing normalized VEGFR-2, sFlt-1, pAkt and pERK in treated samples (Con, control; sFlt-1, sFlt-1 protein; alpha -Flt, Flt-1 neutralizing antibody; n = 3, *P<0.05). All values are represented as the means±SEM. B: Representative VEGFR-2 immunoblot of first trimester placental villous explants treated with VEGF. Densitometry analyses comparing normalized VEGFR-2 between control and VEGF-treated samples (Con, control; VEGF, VEGF-treated; n = 3, *P<0.05). All values are represented as the means±SEM. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24260556), licensed under a CC-BY license. Not internally tested by R&D Systems.

An inverse correlation between VEGFR-2 and sFlt-1 levels in preeclamptic placentae.A: Representative VEGFR-2 and sFlt-1 immunoblots of PE and PTC placentae. Densitometry analysis comparing normalized VEGFR-2 immunoreactivity between PE (n = 13) and PTC (n = 8) samples. VEGFR-2 transcript levels were assessed by qPCR analysis comparing PE (n = 12) and PTC (n = 7) samples. B: Representative VEGFR-2 and sFlt-1 immunoblots of placental tissues from PE and control (Con) dichorionic twins. (PE, preeclampsia; PTC, age-matched preterm control; *P<0.05). All values are represented as the means±SEM. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24260556), licensed under a CC-BY license. Not internally tested by R&D Systems.

Effect of the metalloprotease inhibitors, GM6001 and TAPI-1 on Flt1 N-terminal cleavage. Panel A: HUVECs were incubated with GM6001 (10 µg/ml) and PMA (30 nM) for the indicated times. GM6001 significantly reduces the PMA-induced soluble Flt1 levels measured by ELISA in conditioned media (CM). **p<0.001 and *p<0.05, n = 3. Panel B and C: HEK293 cells transiently expressing HA and Flag-tagged Flt1 were treated with metalloproteases inhibitors, GM6001 (10 µg/ml) and TAPI-1 (20 µM) and conditioned media was immunoblotted with HA, the epitope tag at the N-terminal end of Flt1 or with AF321, an antibody that recognizes the N-terninus of Flt1 and/or sFlt1. Both GM6001 and TAPI-1 significantly reduces the abundance of the cleaved N-terminal fragment. Representative immunoblot in B and pooled data quantified by densitometry shown in C. **p<0.001 by Kruskal Wallis ANOVAR, Mean ± SE, n = 3–7. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/25387128), licensed under a CC0-1.0 license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF321U-100UG
Alexa Fluor 405	AF321V-100UG
Alexa Fluor 488	AF321G-100UG
Alexa Fluor 532	AF321X-100UG
Alexa Fluor 594	AF321T-100UG
Alexa Fluor 647	AF321R-100UG
Alexa Fluor 700	AF321N-100UG
Alexa Fluor 750	AF321S-100UG
Alexa Fluor Plus 405	AF321AFP405-100UG
Alexa Fluor Plus 488	AF321AFP488-100UG
Alexa Fluor Plus 555	AF321AFP555-100UG
Alexa Fluor Plus 594	AF321AFP594-100UG
Alexa Fluor Plus 647	AF321AFP647-100UG
Alexa Fluor Plus 680	AF321AFP680-100UG
Allophycocyanin	AF321A
Biotin	BAF321
CoraFluor 1	AF321CL1
DyLight 350	AF321D
DyLight 405	AF321E
DyLight 488	AF321K
DyLight 550	AF321L
DyLight 594	AF321M
DyLight 650	AF321W
DyLight 680	AF321Y
DyLight 755	AF321Z
FITC	AF321F
HRP	AF321H
Janelia Fluor 525	AF321JF525
Janelia Fluor 549	AF321I
Janelia Fluor 646	AF321J
Janelia Fluor 669	AF321JF669
PE	AF321P
PerCP	AF321C
mFluor Violet 450 SE	AF321MFV450
mFluor Violet 500 SE	AF321MFV500
mFluor Violet 610 SE	AF321MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF321-SP

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DVR100C

Human VEGFR1/Flt-1 Quantikine ELISA Kit, NEW

(90 Publications)

1 Kit

DVR100C

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Conjugate	Catalog #	Availability	Size	Price
HRP	PDVR100C
HRP	SVR100C

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	PDVR100C
Carrier Free	SVR100C

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AF471

VEGFR1/Flt-1 Antibody [Unconjugated]

(37 Publications)

100 μg

AF471

493-MV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse VEGF₁₆₄(5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse VEGFR1/Flt-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF471). The ND₅₀ is typically 2-8 µg/mL." class="big_lightbox">

Recombinant Mouse VEGF<sub>164</sub> <a class=

493-MV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse VEGF₁₆₄(5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse VEGFR1/Flt-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF471). The ND₅₀ is typically 2-8 µg/mL." alt="Recombinant Mouse VEGF₁₆₄ 493-MV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse VEGF₁₆₄(5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse VEGFR1/Flt-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF471). The ND₅₀ is typically 2-8 µg/mL." class="big_thumb" />

Western blot analysis shows lower VEGFR1 and VEGFR2 expression in emphysematous tissues compared to controls. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/19930612), licensed under a CC-BY license. Not internally tested by R&D Systems.

VEGFR-1, CD31, F4/80, and CD11b expression in the pre-implantation mouse uterus. IHC and double staining IF were performed on E3.5 uterine cross-sections. (A) Schematic representation of an E3.5 mouse uterus showing lumen (arrowheads), glands, stroma (s), and myometrium (myo). (B) ECs, detected by CD31 staining (brown), are observed throughout the stroma and myometrium, similar to the non-pregnant state. (C) Macrophages, detected by F4/80 staining (brown), are observed throughout the stroma and are abundant adjacent to the lumen and glands at E3.5. (D) VEGFR-1+ cells (brown), are distributed throughout the stroma and cell associated VEGFR-1 expression highlighted in the inset. (E) Double staining for VEGFR-1 (red) and CD31 (green) demonstrates expression of VEGFR-1 on CD31+ ECs throughout the stroma. (F) VEGFR-1+ cells (red) and F4/80+ macrophages (green) are distributed throughout the stroma. VEGFR-1 and F4/80 co-expression is not observed. (G) VEGFR-1+ cells (red) and CD11b+ monocytes (green) are distributed throughout the stroma. VEGFR-1 and CD11b co-expression is not observed. L, lumen. Scale bars B, C = 100 μm. Scale bars D – F = 50 μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25101167), licensed under a CC-BY license. Not internally tested by R&D Systems.

VEGFR-1 expression in endothelial cells and macrophages in the post-implantation uterus. H&E and double staining IF were performed on E6.5 frontal uterine sections. (A) H&E of a post-implantation mouse uterus showing the embryo (e), anti-mesometrial (am) and mesometrial (m) areas. (B-F) VEGFR-1+ cells (red) are observed in the decidua, with abundant expression in the primary decidual zone surrounding the implanted embryo. (B) Double-staining for VEGFR-1+ (red) and CD31+ (green) cells demonstrates expression of VEGFR-1 in a subset of CD31+ ECs. (C) Double-staining for VEGFR-1+ (red) and endomucin+ (green) cells demonstrates expression of VEGFR-1 in a subset of endomucin+ ECs. (D) Double-staining for VEGFR-1+ (red) and VE-cadherin+ (green) cells demonstrates expression of VEGFR-1 in VE-cadherin+ ECs. (E) Double-staining for VEGFR-1+ (red) and CD11b+ (green) cells demonstrates that VEGFR-1 is not expressed in CD11b+ monocytes. (F) Double-staining for VEGFR-1+ (red) and F4/80+ (green) cells demonstrates that VEGFR-1 is not expressed in F4/80+ macrophages. VEGFR-1+ cells are adjacent to CD11b+ monocytes and F4/80+ macrophages. White boxes in (B-F) indicate areas of the uteri magnified below (B1-F1). (A-F) Scale bar = 500 μm. (B1-F1) Scale bar = 20 μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25101167), licensed under a CC-BY license. Not internally tested by R&D Systems.

Tumor vascularization and metastasis are enhanced in beta IV‐ECKO mice. (A, B) Immunoblots show the level of endogenous beta IV‐spectrin along with the indicated receptor levels and activation of VEGF signaling effectors in freshly isolated primary ECs from control versus beta IV‐ECKO tumors. Graphs represent densitometry quantifications based on three biological repeats normalized to beta ‐actin loading control. p values are indicated in each graph. (C) Shown are representative IHC images of tumor sections stained with CD31 (indicated by black arrows). (D, E) Graphs show the number of vessels counted per mouse tumor section and the cross‐section area of individual blood vessels (n = 6 mice for control and beta IV‐ECKO). * p = 0.003 relative to control group. (F) Shown are representative images of lung and brain isolated from control and beta IV‐ECKO mice at necropsy. (G) Graph represents the average number of metastatic tumor nodules on the surface of different tissues per mouse (n = 7 mice for control and n = 8 for beta IV‐ECKO mice). Error bars represented by SEM. *p < 0.04 relative to control. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37680049), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF471U-100UG
Alexa Fluor 405	AF471V-100UG
Alexa Fluor 488	AF471G-100UG
Alexa Fluor 532	AF471X-100UG
Alexa Fluor 594	AF471T-100UG
Alexa Fluor 647	AF471R-100UG
Alexa Fluor 700	AF471N-100UG
Alexa Fluor 750	AF471S-100UG
Alexa Fluor Plus 405	AF471AFP405-100UG
Alexa Fluor Plus 488	AF471AFP488-100UG
Alexa Fluor Plus 555	AF471AFP555-100UG
Alexa Fluor Plus 594	AF471AFP594-100UG
Alexa Fluor Plus 647	AF471AFP647-100UG
Alexa Fluor Plus 680	AF471AFP680-100UG
Allophycocyanin	AF471A
Biotin	BAF471
CoraFluor 1	AF471CL1
DyLight 350	AF471D
DyLight 405	AF471E
DyLight 488	AF471K
DyLight 550	AF471L
DyLight 594	AF471M
DyLight 650	AF471W
DyLight 680	AF471Y
DyLight 755	AF471Z
FITC	AF471F
HRP	AF471H
Janelia Fluor 525	AF471JF525
Janelia Fluor 549	AF471I
Janelia Fluor 646	AF471J
Janelia Fluor 669	AF471JF669
PE	AF471P
PerCP	AF471C
mFluor Violet 450 SE	AF471MFV450
mFluor Violet 500 SE	AF471MFV500
mFluor Violet 610 SE	AF471MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF471-SP

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321-FL

Recombinant Human VEGFR1/Flt-1 Fc Chimera Protein

(42 Publications)

50 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	321-FL-050/CF

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MVR100

Mouse VEGFR1/Flt-1 Quantikine ELISA Kit

(39 Publications)

1 Kit

MVR100

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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471-F1

Recombinant Mouse VEGFR1/Flt-1 Fc Chimera Protein, CF

(27 Publications)

100 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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7756-FL

Recombinant Mouse VEGFR1/Flt-1 Fc Chimera Protein, CF

(4 Publications)

50 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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NB100-527

VEGFR1/Flt-1 Antibody - BSA Free

(5 Publications)

0.1 ml

NB100-527

Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody [NB100-527] - Analysis of FFPE human breast carcinoma tissue section using 1:500 dilution of VEGFR1/Flt-1 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This VEGFR1/Flt-1 antibody generated an expected membrane cytoplasmic staining of VEGFR1 protein in the cancer cells (punctate appearance typical of receptors). The tumor stroma/stromal cells did not show VEGFR1/Flt-1immunopositivity.

Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody [NB100-527] - Analysis of a FFPE human breast carcinoma tissue section using 1:500 dilution of VEGFR1/Flt-1 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This VEGFR1/Flt-1 antibody generated an expected membrane cytoplasmic staining of VEGFR1 protein in the cancer cells. The tumor stroma/stromal cells did not show VEGFR1/Flt-1immunopositivity. Staining was performed by Histowiz.

VEGFR1 overexpression in K562 cells. Image from a verified customer review.

Reactivity:

Hu, Mu, Rt, Ch

Species Glossary

Reactivity: Hu, Mu, Rt, Ch

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-527AF350
Alexa Fluor 405	NB100-527AF405
Alexa Fluor 488	NB100-527AF488
Alexa Fluor 532	NB100-527AF532
Alexa Fluor 594	NB100-527AF594
Alexa Fluor 647	NB100-527AF647
Alexa Fluor 700	NB100-527AF700
Alexa Fluor 750	NB100-527AF750
Alexa Fluor Plus 405	NB100-527AFP405
Alexa Fluor Plus 488	NB100-527AFP488
Alexa Fluor Plus 555	NB100-527AFP555
Alexa Fluor Plus 594	NB100-527AFP594
Alexa Fluor Plus 647	NB100-527AFP647
Alexa Fluor Plus 680	NB100-527AFP680
Allophycocyanin	NB100-527APC
Biotin	NB100-527B
CoraFluor 1	NB100-527CL1
DyLight 350	NB100-527UV
DyLight 405	NB100-527V
DyLight 488	NB100-527G
DyLight 550	NB100-527R
DyLight 594	NB100-527DL594
DyLight 650	NB100-527C
DyLight 680	NB100-527FR
DyLight 755	NB100-527IR
FITC	NB100-527F
HRP	NB100-527H
Janelia Fluor 525	NB100-527JF525
Janelia Fluor 549	NB100-527JF549
Janelia Fluor 646	NB100-527JF646
Janelia Fluor 669	NB100-527JF669
PE	NB100-527PE
PerCP	NB100-527PCP
mFluor Violet 450 SE	NB100-527MFV450
mFluor Violet 500 SE	NB100-527MFV500
mFluor Violet 610 SE	NB100-527MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-527SS

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MAB321

VEGFR1/Flt-1 Antibody (49560) [Unconjugated]

(14 Publications)

100 μg

MAB321

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." class="big_lightbox">

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." alt="HUVEC human umbilical vein endothelial cells was stained with Mouse Anti-Human VEGFR1/Flt-1 Monoclonal Antibody (Catalog # MAB321, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB321U-100UG
Alexa Fluor 405	FAB321V-100UG
Alexa Fluor 488	FAB321G-100UG
Alexa Fluor 532	FAB321X
Alexa Fluor 594	FAB321T-100UG
Alexa Fluor 647	FAB321R-100UG
Alexa Fluor 700	FAB321N-100UG
Alexa Fluor 750	FAB321S-100UG
Alexa Fluor Plus 405	FAB321AFP405-100UG
Alexa Fluor Plus 488	FAB321AFP488-100UG
Alexa Fluor Plus 555	FAB321AFP555-100UG
Alexa Fluor Plus 594	FAB321AFP594-100UG
Alexa Fluor Plus 647	FAB321AFP647-100UG
Alexa Fluor Plus 680	FAB321AFP680-100UG
Allophycocyanin	FAB321A
Allophycocyanin/Cy7	FAB321APCCY7
Biotin	FAB321B
CoraFluor 1	FAB321CL1
DyLight 350	FAB321D
DyLight 405	FAB321E
DyLight 488	FAB321K
DyLight 550	FAB321L
DyLight 594	FAB321M
DyLight 650	FAB321W
DyLight 680	FAB321Y
DyLight 755	FAB321Z
FITC	FAB321F
HRP	FAB321H
Janelia Fluor 525	FAB321JF525
Janelia Fluor 549	FAB321I
Janelia Fluor 646	FAB321J
Janelia Fluor 669	FAB321JF669
PE/Atto594	FAB321PEATT594
PE/Cy5.5	FAB321PECY55
PE/Cy7	FAB321PECY7
PerCP	FAB321C
Phycoerythrin	FAB321P
mFluor Violet 450 SE	FAB321MFV450
mFluor Violet 500 SE	FAB321MFV500
mFluor Violet 610 SE	FAB321MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB321P
Carrier Free	FAB321A
Carrier Free	MAB321-SP
Carrier Free	FAB321V-100UG
Carrier Free	FAB321U-100UG
Carrier Free	FAB321T-100UG
Carrier Free	FAB321S-100UG
Carrier Free	FAB321R-100UG
Carrier Free	FAB321N-100UG
Carrier Free	FAB321G-100UG

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AF4170

VEGFR1/Flt-1 [p Tyr1213] Antibody [Unconjugated]

(14 Publications)

100 μg

AF4170

HAF008). A specific band was detected for Phospho-VEGFR1/Flt-1 (Y1213) at approximately 130-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates of HUVEC human umbilical vein endothelial cell untreated (-) or treated (+) with 100 µM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Phospho-VEGFR1/Flt-1 (Y1213) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4170), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # <a class=

HAF008). A specific band was detected for Phospho-VEGFR1/Flt-1 (Y1213) at approximately 130-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HUVEC human umbilical vein endothelial cell untreated (-) or treated (+) with 100 µM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Phospho-VEGFR1/Flt-1 (Y1213) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4170), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-VEGFR1/Flt-1 (Y1213) at approximately 130-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF4170U-100UG
Alexa Fluor 405	AF4170V-100UG
Alexa Fluor 488	AF4170G-100UG
Alexa Fluor 532	AF4170X-100UG
Alexa Fluor 594	AF4170T-100UG
Alexa Fluor 647	AF4170R-100UG
Alexa Fluor 700	AF4170N-100UG
Alexa Fluor 750	AF4170S-100UG
Alexa Fluor Plus 405	AF4170AFP405-100UG
Alexa Fluor Plus 488	AF4170AFP488-100UG
Alexa Fluor Plus 555	AF4170AFP555-100UG
Alexa Fluor Plus 594	AF4170AFP594-100UG
Alexa Fluor Plus 647	AF4170AFP647-100UG
Alexa Fluor Plus 680	AF4170AFP680-100UG
Allophycocyanin	AF4170A
Biotin	AF4170B
CoraFluor 1	AF4170CL1
DyLight 350	AF4170D
DyLight 405	AF4170E
DyLight 488	AF4170K
DyLight 550	AF4170L
DyLight 594	AF4170M
DyLight 650	AF4170W
DyLight 680	AF4170Y
DyLight 755	AF4170Z
FITC	AF4170F
HRP	AF4170H
Janelia Fluor 525	AF4170JF525
Janelia Fluor 549	AF4170I
Janelia Fluor 646	AF4170J
Janelia Fluor 669	AF4170JF669
PE	AF4170P
PerCP	AF4170C
mFluor Violet 450 SE	AF4170MFV450
mFluor Violet 500 SE	AF4170MFV500
mFluor Violet 610 SE	AF4170MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF4170-SP

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NB600-1004

VEGFR1/Flt-1 Antibody (EWC) - Azide and BSA Free

(3 Reviews)

(3 Publications)

0.1 mg

NB600-1004

Western Blot: VEGFR1/Flt-1 Antibody (Flt-1/EWC) [NB600-1004] - Analysis of recombinant mouse soluble VEGFR-1 conditioned supernatant using a monoclonal antibody directed against human recombinant sFlt-1(D5). There is a strong cross reactivity with the mouse sFlt-1 visible.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB600-1004AF350
Alexa Fluor 405	NB600-1004AF405
Alexa Fluor 488	NB600-1004AF488
Alexa Fluor 532	NB600-1004AF532
Alexa Fluor 594	NB600-1004AF594
Alexa Fluor 647	NB600-1004AF647
Alexa Fluor 700	NB600-1004AF700
Alexa Fluor 750	NB600-1004AF750
Alexa Fluor Plus 405	NB600-1004AFP405
Alexa Fluor Plus 488	NB600-1004AFP488
Alexa Fluor Plus 555	NB600-1004AFP555
Alexa Fluor Plus 594	NB600-1004AFP594
Alexa Fluor Plus 647	NB600-1004AFP647
Alexa Fluor Plus 680	NB600-1004AFP680
Allophycocyanin	NB600-1004APC
Biotin	NBP1-18636
CoraFluor 1	NB600-1004CL1
DyLight 350	NB600-1004UV
DyLight 405	NB600-1004V
DyLight 488	NB600-1004G
DyLight 550	NB600-1004R
DyLight 594	NB600-1004DL594
DyLight 650	NB600-1004C
DyLight 680	NB600-1004FR
DyLight 755	NB600-1004IR
FITC	NB600-1004F
HRP	NB600-1004H
Janelia Fluor 525	NB600-1004JF525
Janelia Fluor 549	NB600-1004JF549
Janelia Fluor 646	NB600-1004JF646
Janelia Fluor 669	NB600-1004JF669
PE	NB600-1004PE
PerCP	NB600-1004PCP
mFluor Violet 450 SE	NB600-1004MFV450
mFluor Violet 500 SE	NB600-1004MFV500
mFluor Violet 610 SE	NB600-1004MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NB600-1004-20ug

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NBP3-11864

VEGFR1/Flt-1 Antibody (SY09-09)

100 ul

NBP3-11864

Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using NBP3-11864. Counter stained with hematoxylin.

Flow Cytometry: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Flow cytometric analysis of A431 cells with NBP3-11864 at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Immunocytochemistry staining VEGFR1/Flt-1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Immunocytochemistry staining VEGFR1/Flt-1 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using NBP3-11864. Counter stained with hematoxylin.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

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