NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="Fc gamma RII/CD32 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human Fc gamma RII/CD32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1330) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1330-SP | |||
F0107). View our protocol for Staining Membrane-associated Proteins. " alt="Whole blood granulocytes were stained with Goat Anti-Human Fc gamma RII/CD32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1875, filled histogram) or isotype control antibody (Catalog # 4-001-A, open histogram) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1875-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-65338-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB14601-SP | |||
| Carrier Free | MAB14601-500 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB18751-SP | |||
| Carrier Free | MAB18751-500 | |||
![Flow Cytometry: Fc gamma RII/CD32 Antibody (AT10) [NB100-65338] - Analysis using the FITC conjugate of NB100-65338. Staining of human peripheral blood granulocytes.](http://images.novusbio.com/fullsize/Fc-gamma-RII-CD32-Antibody-AT10-Flow-Cytometry-NB100-65338-img0005.jpg "Flow Cytometry: Fc gamma RII/CD32 Antibody (AT10) [NB100-65338] - Analysis using the FITC conjugate of NB100-65338. Staining of human peripheral blood granulocytes.")
![Flow Cytometry: Fc gamma RII/CD32 Antibody (AT10) [NB100-65338] - Analysis using the Biotin conjugate of NB100-65338. Staining of human peripheral blood monocytes with mouse anti human CD32: BIOTIN.](http://images.novusbio.com/fullsize/Fc-gamma-RII-CD32-Antibody-AT10-Flow-Cytometry-NB100-65338-img0004.jpg)
![Western Blot: Fc gamma RIIB/CD32b Antibody [NBP2-14905] - Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Fc-gamma-RIIB-CD32b-Antibody-Western-Blot-NBP2-14905-img0005.jpg "Western Blot: Fc gamma RIIB/CD32b Antibody [NBP2-14905] - Image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: Fc gamma RIIB/CD32b Antibody [NBP2-14905] - Analysis of methanol-fixed A431, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Fc-gamma-RIIB-CD32b-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-14905-img0003.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Fc gamma RIIB/CD32b Antibody [NBP2-14905] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CD32B antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-14905_rabbit-polyclonal-fc-gamma-riib-cd32b-antibody-1610202419405774.jpg)
![Western Blot: Fc gamma RII/CD32 Antibody [NB100-1532] - (1ug/ml) staining of Daudi cell lysate (RIPA buffer, (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Fc-gamma-RII-CD32-Antibody-Western-Blot-NB100-1532-img0006.jpg "Western Blot: Fc gamma RII/CD32 Antibody [NB100-1532] - (1ug/ml) staining of Daudi cell lysate (RIPA buffer, (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Fc gamma RII/CD32 Antibody [NB100-1532] - Staining (8ug/ml) of paraffin embedded Human Placenta. Heat induced antigen retrieval with citrate buffer pH 6, HRP-Staining.](http://images.novusbio.com/fullsize/Fc-gamma-RII-CD32-Antibody-Immunohistochemistry-Paraffin-NB100-1532-img0007.jpg)
![Immunohistochemistry-Paraffin: Fc gamma RII/CD32 Antibody [NB100-1532] - Negative Control showing staining of paraffin embedded Human Placenta, with no primary antibody.](http://images.novusbio.com/fullsize/Fc-gamma-RII-CD32-Antibody-Immunohistochemistry-Paraffin-NB100-1532-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Goat Polyclonal Fc gamma RII/CD32 Antibody [NB100-1532] - Immunofluorescence analysis of paraformaldehyde fixed THP-1 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/antibody/nb100-1532_goat-polyclonal-fc-gamma-rii-cd32-antibody-immunocytochemistry-immunofluorescence-2832024113032..jpg)
![Immunocytochemistry/Immunofluorescence: Fc gamma RIIB/CD32b Antibody (HL1948) - Azide and BSA Free [NBP3-25404] - CD32B antibody [HL1948] detects CD32B protein at cell membrane by immunofluorescent analysis. Sample: U937 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD32B stained by CD32B antibody [HL1948] (NBP3-25404) diluted at 1:1000. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25404_rabbit-fc-gamma-riib-cd32b-mab-hl1948-azide-and-bsa-free-2120249355521.jpg "Immunocytochemistry/Immunofluorescence: Fc gamma RIIB/CD32b Antibody (HL1948) - Azide and BSA Free [NBP3-25404] - CD32B antibody [HL1948] detects CD32B protein at cell membrane by immunofluorescent analysis. Sample: U937 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD32B stained by CD32B antibody [HL1948] (NBP3-25404) diluted at 1:1000. Blue: Fluoroshield with DAPI .")
![Immunocytochemistry/Immunofluorescence: Fc gamma RIIB/CD32b Antibody (HL1948) - Azide and BSA Free [NBP3-25404] - CD32B antibody [HL1948] detects CD32B protein by immunofluorescent analysis. Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD32B stained by CD32B antibody [HL1948] (NBP3-25404) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25404_rabbit-fc-gamma-riib-cd32b-mab-hl1948-azide-and-bsa-free-2120249351351.jpg)
![Immunohistochemistry-Paraffin: Fc gamma RIIB/CD32b Antibody (HL1948) - Azide and BSA Free [NBP3-25404] - CD32B antibody [HL1948] detects CD32B protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. CD32B stained by CD32B antibody [HL1948] (NBP3-25404) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25404_rabbit-fc-gamma-riib-cd32b-mab-hl1948-azide-and-bsa-free-2120249355554.jpg)
![Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CD32B antibody [HL1948] (NBP3-25404) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25404_rabbit-fc-gamma-riib-cd32b-mab-hl1948-azide-and-bsa-free-western-blot-2611202518213323.jpg)
