| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52552-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70692 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38215-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38350-25ul | |||
![Western Blot: eRF1 Antibody [NBP1-31113] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with eRF1 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/eRF1-Antibody-Western-Blot-NBP1-31113-img0010.jpg "Western Blot: eRF1 Antibody [NBP1-31113] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with eRF1 antibody diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: eRF1 Antibody [NBP1-31113] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: eRF1 protein stained by eRF1 antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/eRF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31113-img0008.jpg)
![Immunohistochemistry-Paraffin: eRF1 Antibody [NBP1-31113] - Paraffin-embedded mouse testis. eRF1 antibody diluted at 1:500. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min](http://images.novusbio.com/fullsize/eRF1-Antibody-Immunohistochemistry-Paraffin-NBP1-31113-img0006.jpg)
![Western Blot: eRF1 Antibody [NBP1-31113] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with eRF1 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/eRF1-Antibody-Western-Blot-NBP1-31113-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: eRF1 Antibody [NBP1-31113] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: eRF1 protein stained by eRF1 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/eRF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31113-img0007.jpg)
![Immunohistochemistry-Paraffin: eRF1 Antibody [NBP1-31113] - Paraffin-embedded SW480 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/eRF1-Antibody-Immunohistochemistry-Paraffin-NBP1-31113-img0004.jpg)
![Immunohistochemistry-Paraffin: eRF1 Antibody [NBP1-31113] - Paraffin-embedded mouse prostate. eRF1 antibody diluted at 1:500. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min](http://images.novusbio.com/fullsize/eRF1-Antibody-Immunohistochemistry-Paraffin-NBP1-31113-img0005.jpg)
![Western Blot: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552] - Effect of CC-885 and CC-90009 on COL17A1 PTC readthrough. JEB01 keratinocytes derived from a JEB patient with a COL17A1 nonsense mutation (R688X/R688X) were exposed to the indicated concentrations of CC-90009 for 72 h and eRF3a, eRF3b and eRF1 (NBP2-52552) levels were determined using automated capillary electrophoresis western analysis. GAPDH was used as loading control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33764477/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/eRF1-Antibody-4F9H12-BSA-Free-Western-Blot-NBP2-52552-img0008.jpg "Western Blot: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552] - Effect of CC-885 and CC-90009 on COL17A1 PTC readthrough. JEB01 keratinocytes derived from a JEB patient with a COL17A1 nonsense mutation (R688X/R688X) were exposed to the indicated concentrations of CC-90009 for 72 h and eRF3a, eRF3b and eRF1 (NBP2-52552) levels were determined using automated capillary electrophoresis western analysis. GAPDH was used as loading control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33764477/) licensed under a CC-BY license.")
![Flow Cytometry: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552] - Analysis of HepG2 cells using RF1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/eRF1-Antibody-4F9H12-BSA-Free-Flow-Cytometry-NBP2-52552-img0005.jpg)
![Western Blot: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552] - Analysis using RF1 mAb against HEK293 (1) and RF1 (AA: 288-437)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/eRF1-Antibody-4F9H12-BSA-Free-Western-Blot-NBP2-52552-img0004.jpg)
![Western Blot: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552] - Analysis using RF1 mAb against human RF1 (AA: 288-437) recombinant protein. (Expected MW is 43 kDa)](http://images.novusbio.com/fullsize/eRF1-Antibody-4F9H12-BSA-Free-Western-Blot-NBP2-52552-img0003.jpg)
![Western Blot: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552] - Analysis using eRF1 mouse mAb against MCF-7 (1), T47D (2), MOLT4 (3), and Raji (4) cell lysate.](http://images.novusbio.com/fullsize/eRF1-Antibody-4F9H12-BSA-Free-Western-Blot-NBP2-52552-img0006.jpg)
![Flow Cytometry: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552] - Analysis of Hela cells using RF1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/eRF1-Antibody-4F9H12-BSA-Free-Flow-Cytometry-NBP2-52552-img0001.jpg)
![ELISA: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng).](http://images.novusbio.com/fullsize/eRF1-Antibody-4F9H12-BSA-Free-ELISA-NBP2-52552-img0007.jpg)
![Simple Western: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552] - Effect of CC-885 & CC-90009 on COL17A1 PTC readthrough. (A, B) HaCaT keratinocytes derived from an unaffected individual were exposed to various concentrations of CC-885 (A) or CC-90009 (B) with or without 100 μg/ml gentamicin sulfate for 48 h & cell viability was measured using the MTT assay in triplicate samples (±SD). Concentrations of CC-885 & CC-90009 are in nanomolar. (C, D) JEB01 keratinocytes derived from a JEB patient with a COL17A1 nonsense mutation (R688X/R688X) were exposed to the indicated concentrations of CC-885 (C) or CC-90009 (D) for 72 h & eRF3a, eRF3b & eRF1 levels were determined using automated capillary electrophoresis western analysis. GAPDH was used as loading control. (E, F) JEB01 keratinocytes were exposed to the indicated concentrations of CC-885 (E) or CC-90009 (F) in the presence or absence of 100 or 300 μg/ml gentamicin sulfate for 72 h & Collagen XVII levels were measured by SDS-PAGE followed by traditional western blotting. beta -actin was used as loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33764477), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-52552_mouse-monoclonal-erf1-antibody-4f9h12-310202416121930.jpg)
![Western Blot: eRF1 Antibody (OTI2E3) - Azide and BSA Free [NBP2-70692] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY eRF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-eRF1.](http://images.novusbio.com/fullsize/eRF1-Antibody-OTI2E3-Azide-and-BSA-Free-Western-Blot-NBP2-70692-img0002.jpg "Western Blot: eRF1 Antibody (OTI2E3) - Azide and BSA Free [NBP2-70692] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY eRF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-eRF1.")
![Flow Cytometry: eRF1 Antibody (OTI2E3) - Azide and BSA Free [NBP2-70692] - Analysis of Jurkat cells, using anti-eRF1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/eRF1-Antibody-OTI2E3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70692-img0004.jpg)
![Western Blot: eRF1 Antibody (OTI2E3) - Azide and BSA Free [NBP2-70692] - Analysis of extracts (35ug) from 9 different cell lines by using anti-eRF1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549:human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)](http://images.novusbio.com/fullsize/eRF1-Antibody-OTI2E3-Azide-and-BSA-Free-Western-Blot-NBP2-70692-img0001.jpg)
![Flow Cytometry: eRF1 Antibody (OTI2E3) - Azide and BSA Free [NBP2-70692] - Analysis of Hela cells, using anti-eRF1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/eRF1-Antibody-OTI2E3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70692-img0003.jpg)
![Western Blot: eRF1 Antibody [NBP1-31144] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE eRF1 antibody, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/eRF1-Antibody-Western-Blot-NBP1-31144-img0003.jpg "Western Blot: eRF1 Antibody [NBP1-31144] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE eRF1 antibody, antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: eRF1 Antibody [NBP1-31144] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/eRF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31144-img0002.jpg)
![Immunohistochemistry-Paraffin: eRF1 Antibody [NBP1-31144] - Sample: Paraffin-embedded mouse brain. eRF1 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/eRF1-Antibody-Immunohistochemistry-Paraffin-NBP1-31144-img0004.jpg)
![Immunohistochemistry: eRF1 Antibody [NBP1-31144] - eRF1 antibody detects eRF1 protein on myofiber membranes by immunohistochemical analysis Sample: Agarose-embedded zebrafish embryo eRF1 antibody (NBP1-31144) dilution: 1:200. Image provided with permission courtesy of Dr. T. Schilling at UC, Irvine.](http://images.novusbio.com/fullsize/nbp1-31144_rabbit-polyclonal-erf1-antibody-910202419593592.jpg)
![Western Blot: eRF1 Antibody (2E3) [NBP2-00740] - Analysis of extracts (35ug) from 9 different cell lines by using anti-eRF1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549:human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)](http://images.novusbio.com/fullsize/eRF1-Antibody-2E3-Western-Blot-NBP2-00740-img0011.jpg "Western Blot: eRF1 Antibody (2E3) [NBP2-00740] - Analysis of extracts (35ug) from 9 different cell lines by using anti-eRF1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549:human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)")
![Flow Cytometry: eRF1 Antibody (2E3) [NBP2-00740] - Analysis of Jurkat cells, using anti-eRF1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/eRF1-Antibody-2E3-Flow-Cytometry-NBP2-00740-img0005.jpg)
![Western Blot: eRF1 Antibody (2E3) [NBP2-00740] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY eRF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-eRF1.](http://images.novusbio.com/fullsize/eRF1-Antibody-2E3-Western-Blot-NBP2-00740-img0006.jpg)
![Flow Cytometry: eRF1 Antibody (2E3) [NBP2-00740] - Analysis of Hela cells, using anti-eRF1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/eRF1-Antibody-2E3-Flow-Cytometry-NBP2-00740-img0004.jpg)
![Western Blot: eRF1 Antibody [NBP1-57565] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:62500 Positive Control: THP - 1 cell lysate](http://images.novusbio.com/fullsize/eRF1-Antibody-Western-Blot-NBP1-57565-img0004.jpg "Western Blot: eRF1 Antibody [NBP1-57565] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:62500 Positive Control: THP - 1 cell lysate")
![Immunohistochemistry: eRF1 Antibody [NBP1-57565] - Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue Observed Staining: Cytoplasm in bile ductule Primary Antibody Concentration: 1:100 Other Working Concentrations: 1:600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec](http://images.novusbio.com/fullsize/eRF1-Antibody-Immunohistochemistry-NBP1-57565-img0003.jpg)
![Western Blot: eRF1 Antibody [NBP3-38215] - Western blot analysis of various lysates using eRF1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-38215_rabbit-erf1-pab-10122024126233.jpg "Western Blot: eRF1 Antibody [NBP3-38215] - Western blot analysis of various lysates using eRF1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.")
![Immunocytochemistry/ Immunofluorescence: eRF1 Antibody [NBP3-38215] - Immunofluorescence analysis of HeLa cells using eRF1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38215_rabbit-erf1-pab-1012202411545571.jpg)
![Western Blot: eRF1 Antibody [NBP2-38350] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma](http://images.novusbio.com/fullsize/eRF1-Antibody-Western-Blot-NBP2-38350-img0002.jpg "Western Blot: eRF1 Antibody [NBP2-38350] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma")
![Immunohistochemistry: eRF1 Antibody [NBP2-38350] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/eRF1-Antibody-Immunohistochemistry-NBP2-38350-img0001.jpg)
![Western Blot: eRF1 Antibody (2H4) [H00002107-M02] - Analysis of ETF1 expression in transfected 293T cell line by ETF1 monoclonal antibody (M02), clone 2H4.Lane 1: ETF1 transfected lysate (Predicted MW: 49 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/eRF1-Antibody-2H4-Western-Blot-H00002107-M02-img0005.jpg "Western Blot: eRF1 Antibody (2H4) [H00002107-M02] - Analysis of ETF1 expression in transfected 293T cell line by ETF1 monoclonal antibody (M02), clone 2H4.Lane 1: ETF1 transfected lysate (Predicted MW: 49 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: eRF1 Antibody (2H4) [H00002107-M02] - ETF1 monoclonal antibody (M02), clone 2H4. Analysis of ETF1 expression in HepG2.](http://images.novusbio.com/fullsize/eRF1-Antibody-2H4-Western-Blot-H00002107-M02-img0004.jpg)
![Sandwich ELISA: eRF1 Antibody (2H4) [H00002107-M02] - Detection limit for recombinant GST tagged ETF1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/eRF1-Antibody-2H4-Sandwich-ELISA-H00002107-M02-img0003.jpg)
![SDS-Page: Recombinant Human eRF1 Protein [H00002107-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00002107-P01-1.jpg "SDS-Page: Recombinant Human eRF1 Protein [H00002107-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: eRF1 Overexpression Lysate (Adult Normal) [NBL1-10348] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for eRF1.](http://images.novusbio.com/fullsize/eRF1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10348-img0002.jpg "Western Blot: eRF1 Overexpression Lysate (Adult Normal) [NBL1-10348] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for eRF1.")