AHR: Novus Biologicals

NB100-128

AHR Antibody - Azide and BSA Free

(1 Review)

(19 Publications)

1 ml

NB100-128

Gel Super Shift Assays: AHR Antibody [NB100-128] - EMSA validation of AHR antibody on U937 cells nuclear protein. Image submitted by verified customer - see review for protocol details.

AHR agonist activates the SPHK2/S1P pathway resulting in rapid nuclear retention of the AHR and SPHK2. A) Immunofluorescent images of HeLa cells for p‐SPHK2 levels after TCDF (0.1 uM) incubation for 20 min. Arrowheads indicate the peri‐nuclear region staining of p‐SPHK2 in control cells and arrows indicate the nuclear expression of p‐SPHK2 in TCDF treated cells. Hoechst 33342 was used for the counter staining of nuclei. Bar = 20 um. Data are representative images from three independent experiments. B) Western blot analysis of HeLa cells after TCDF treatment for 15 min. C, Control; T, TCDF (n = 3). C) qRT‐PCR analysis of HeLa cells for AHR and SPHK2 mRNA levels after S1P incubation for 30 min. C: Control. Data are mean +/- S.D. (n = 3). ****p < 0.0001, *p < 0.05. D) Western blotting analysis of HeLa cells after S1P treatment for 5 min. Cytoplasmic and nuclear extracts were obtained and western blotting analysis conducted with the antibodies indicated (n = 3). E) Western blotting analysis of HeLa cell extracts after 5‐ and 10‐min treatment with S1P (10 uM). GAPDH and histone H3 (H3) antibodies were used as internal controls for cytoplasmic and nuclear extracts, respectively. All data are representative images of three independent experiments (n = 3). F) Pulldown assays using S1P immobilized on agarose beads or equivalent control beads. After extensive washing, bound proteins were dissolved in SDS sample buffer and separated by SDS‐PAGE and subjected to western blotting analysis (n = 3). C, Control; T, TCDF treated, respectively. Images are representative of three independent experiments. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/39207053), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Inhibition of serine palmitoyltransferase with myriocin causes decreased AHR expression. A) Western blotting analysis of HeLa cells after myriocin (uM) treatment for 3 h. SPTLC1 and SPTLC2 antibodies were used for the conformation of myriocin effects for the inhibition under this culture condition (10%FBS/DMEM). B) qPCR analysis using AHR‐ and SPTLC1‐specific primers for 2 or 3 h after myriocin treatment. C) Western blotting analysis of HeLa cell whole lysates after myriocin (uM) treatment under serum free conditions for 1 h. D) CCK8 assay results after overnight culture in 10%FBS/DMEM or 2 h in 0%FBS/DMEM with or without myriocin. All data and images are representative from three independent replicates. ****p < 0.0001, ***p < 0.001, **p < 0.01. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/39207053), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Knockdown of SPHK2 downregulates AHR expression. A) qRT‐PCR analysis of CYP1A1 expression in HeLa cells after incubation with or without TCDF (0.1 uM) for 2 h or pretreatment with SPHK2 inhibitor (ABC, ABC294640) for 2 h. Carrier solvent DMSO was used as a control. B) CCK8 assay results after 2 h incubation with different doses of ABC294640 (uM). C) HeLa cells after treatment with 0.1 uM TCDF for 30 min or pretreatment with ABC294640 for 2 h followed by 0.1 uM TCDF for 30 min, cells were fractionated into cytoplasmic and nuclear extracts and subjected to western blotting analysis with the indicated antibodies. Red arrow indicates the reduced level of AHR protein in the ABC294640 + TCDF treated nucleus. LAMIN A/C antibody and GAPDH antibody were used for the loading control of nuclear and cytoplasmic extracts, respectively. n = 3 D) HeLa cells were transfected with SPHK2 shRNA for 48 h and the efficiency of each shRNA plasmid knockdown was assessed by qRT‐PCR (left) and western blotting (right). n = 3 E) Western blot analysis of HeLa cells transfected with SPHK2 shRNA or shRNA‐scramble (scr) for 48 h. Cells were treated 30 min with or without 0.1 uM TCDF, extracted, and proteins detected with the indicated antibodies. n = 3 F) ABC294640 incubation in HeLa cells for 2 h decreased AHR expression as determined by western blotting analysis. n = 3 G) shRNA for SPHK2 for 48 h decreases AHR mRNA in HeLa cells. (H) Transient overexpression of human SPHK2 for 48 h and qRT‐PCR was performed. All data mean +/- S.D. (n = 3). ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/39207053), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Genistein activates the intestinal AhR pathway in broilers challenged with NE. (A) Western blot of AhR and CYP1A1 in the jejunum of broilers. (B) Quantification of AhR and CYP1A1 protein levels, n = 3. Con, basal diet; Gen40, basal diet supplemented with 40 mg/kg genistein; Gen80, Gen80 diet; Cp, basal diet and Cp infection; Cp+Gen40, Gen40 diet and Cp infection; Cp+Gen80, Gen80 diet and Cp infection. The differences among groups were determined by ANOVA using Duncan’s test. The results are presented as mean +/- SEM. Letter a indicates p < 0.05 vs. Con group; letter b indicates p < 0.05 vs. Cp group. Image collected and cropped by CiteAb from the following open publication (//www.mdpi.com/1422-0067/25/12/6656), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Endogenous AHR and SPHK2 interact in HeLa cells. A,B) HeLa cells treated with or without TCDF were subjected to cytoplasmic/nuclear extract fractionation and immunoprecipitated using AHR antibody and western blotting analysis was performed using an (A) AHR antibody or (B) SPHK2 antibody. C, Control; T, TCDF treated samples. C) After IP of SPHK2, western blotting analysis was performed with AHR, ARNT and SPHK2 antibodies. C, Control; T, TCDF treated samples. D) After IP of ARNT, western blotting analysis was performed with AHR, SPHK2 and ARNT antibodies. C, Control; T, TCDF treated samples. E) Immunofluorescent double staining using anti‐AHR and anti‐SPHK2 antibodies. The nucleus was counterstained with Hoechst 33 342. Arrows c indicate the cytoplasm, n indicate the nucleus. Bar is 10 um. Data are representative images from three independent experiments. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/39207053), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

LNVs activate the AhR/Nrf2 signaling pathway(A) Western blot analysis of AhR (n = 3) and Nrf2 (n = 2) levels in HDF alpha cell line pre-treated with LNVs (10 and 25 μg/mL) for 24 h and then stimulated with UVB irradiation (20 mJ/cm2) for 25 s. Data are represented as mean +/- SD.(B and C) Confocal analysis of AhR (B, red signal) and Nrf2 (C, green signal) levels in HDF alpha cell line untreated, or treated with LNVs (10 and 25 μg/mL) for 24h in the presence of UVB; actin was stained with actin green (in green), nuclei with Hoechst (in blue). Scale bar 50 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37426343), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NB100-128SS

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NB100-2289

AHR Antibody - BSA Free

(2 Reviews)

(11 Publications)

0.1 ml

NB100-2289

Immunocytochemistry/Immunofluorescence: AHR Antibody [NB100-2289] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-AHR at 5 ug/mL overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Western Blot: AHR Antibody [NB100-2289] - Detection of AhR in mouse liver cytosol using NB 100-2289.

Immunocytochemistry/Immunofluorescence: AHR Antibody [NB100-2289] - HepG2 cells were fixed for 10 minutes using 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-AHR at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow Cytometry: AHR Antibody [NB100-2289] - An intracellular stain was performed on U937 cells with NB100-2289 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Immunocytochemistry/Immunofluorescence: AHR Antibody [NB100-2289] - Aryl hydrocarbon Receptor antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Flow Cytometry: AHR Antibody [NB100-2289] - An intracellular stain was performed on U-87 cells with AHR Antibody NB100-2289B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).

Simple Western: AHR Antibody [NB100-2289] - Image shows a specific band for Aryl hydrocarbon receptor in 0.5 mg/mL of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Western Blot: AHR Antibody - BSA Free [NB100-2289] - Activation of AHR/Nrf2 pathway by PFT-alpha . (A) qPCR to detect mRNA level of CYP1A1, NQO1, HO1 & TRXR1 upon 20 h PFT-alpha treatment (20 µM) in MCF7 p53KO cells (upper part) & T47D cells (lower part). Relative expression level of CYP1A1 is shown in log2 scale; relative expression level of NQO1, HO1 & TRXR1 are shown as fold change, both normalized with DMSO treatment. All values represent the mean ± SD of two times independent experiments performed in three replicates. (B) Knock-down efficiency of AHR siRNA as detected by qPCR (upper part) & western blot (lower part). Relative expression level is shown as fold change normalized to scramble siRNA. All values represent the mean ± SD of two independent experiments performed in three replicates. (C) DCF-DA staining of ROS levels upon doxorubicin treatment (1 µM, 8 h) with or without PFT-alpha (20 µM, 12 h pre-treatment) in MCF7 p53KO cells transfected with scramble siRNA & siAHR. For quantification of ROS levels (lower panel), the values are reported as percentage relative to DMSO treatment group. (D) qPCR to detect mRNA level of Nrf2 targets NQO1, HO1 & TRXR1 upon 20 h PFT-alpha treatment (20 µM) in MCF7 p53KO cells transfected with scramble siRNA & siAHR. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31974452), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

AHR was detected in immersion fixed A431 human skin carcinoma cell line using Rabbit anti-AHR Affinity Purified Polyclonal Antibody conjugated to FITC (Catalog # NB100-2289F) (green) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Reactivity:

Hu, Mu, Rt, Gp

Species Glossary

Reactivity: Hu, Mu, Rt, Gp

Applications:

, , , , , , ,

Applications: , , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-2289AF350
Alexa Fluor 405	NB100-2289AF405
Alexa Fluor 488	NB100-2289AF488
Alexa Fluor 532	NB100-2289AF532
Alexa Fluor 594	NB100-2289AF594
Alexa Fluor 647	NB100-2289AF647
Alexa Fluor 700	NB100-2289AF700
Alexa Fluor 750	NB100-2289AF750
Alexa Fluor Plus 405	NB100-2289AFP405
Alexa Fluor Plus 488	NB100-2289AFP488
Alexa Fluor Plus 555	NB100-2289AFP555
Alexa Fluor Plus 594	NB100-2289AFP594
Alexa Fluor Plus 647	NB100-2289AFP647
Alexa Fluor Plus 680	NB100-2289AFP680
Allophycocyanin	NB100-2289APC
Biotin	NB100-2289B
CoraFluor 1	NB100-2289CL1
DyLight 350	NB100-2289UV
DyLight 405	NB100-2289V
DyLight 488	NB100-2289G
DyLight 550	NB100-2289R
DyLight 594	NB100-2289DL594
DyLight 650	NB100-2289C
DyLight 680	NB100-2289FR
DyLight 755	NB100-2289IR
FITC	NB100-2289F
HRP	NB100-2289H
Janelia Fluor 525	NB100-2289JF525
Janelia Fluor 549	NB100-2289JF549
Janelia Fluor 646	NB100-2289JF646
Janelia Fluor 669	NB100-2289JF669
PE	NB100-2289PE
PerCP	NB100-2289PCP
mFluor Violet 450 SE	NB100-2289MFV450
mFluor Violet 500 SE	NB100-2289MFV500
mFluor Violet 610 SE	NB100-2289MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-2289SS

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NB300-515

AHR Antibody (RPT9) - BSA Free

(8 Publications)

100 uL

NB300-515

Flow Cytometry: AHR Antibody (RPT9) [NB300-515] - Analysis of Aryl Hydrocarbon Receptor in NIH/3T3 cells compared to an isotype control (blue).

Western Blot: AHR Antibody (RPT9) [NB300-515] - Analysis of 40 ug of HEK293 lysate overexpressing Aryl Hydrocarbon Receptor (right lane) or empty vector control (left lane) and 10ul of PageRuler Prestained Protein Ladder.

Flow Cytometry: AHR Antibody (RPT9) [NB300-515] - Analysis of Aryl Hydrocarbon Receptor in Hela cells compared to an isotype control (blue).

Flow Cytometry: AHR Antibody (RPT9) [NB300-515] - Analysis of Aryl Hydrocarbon Receptor in PC-3 cells compared to an isotype control (blue).

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , , , ,

Applications: , , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB300-515AF350
Alexa Fluor 405	NB300-515AF405
Alexa Fluor 488	NB300-515AF488
Alexa Fluor 532	NB300-515AF532
Alexa Fluor 594	NB300-515AF594
Alexa Fluor 647	NB300-515AF647
Alexa Fluor 700	NB300-515AF700
Alexa Fluor 750	NB300-515AF750
Alexa Fluor Plus 405	NB300-515AFP405
Alexa Fluor Plus 488	NB300-515AFP488
Alexa Fluor Plus 555	NB300-515AFP555
Alexa Fluor Plus 594	NB300-515AFP594
Alexa Fluor Plus 647	NB300-515AFP647
Alexa Fluor Plus 680	NB300-515AFP680
Allophycocyanin	NB300-515APC
Biotin	NB300-515B
CoraFluor 1	NB300-515CL1
DyLight 350	NB300-515UV
DyLight 405	NB300-515V
DyLight 488	NB300-515G
DyLight 550	NB300-515R
DyLight 594	NB300-515DL594
DyLight 650	NB300-515C
DyLight 680	NB300-515FR
DyLight 755	NB300-515IR
FITC	NB300-515F
HRP	NB300-515H
Janelia Fluor 525	NB300-515JF525
Janelia Fluor 549	NB300-515JF549
Janelia Fluor 646	NB300-515JF646
Janelia Fluor 669	NB300-515JF669
PE	NB300-515PE
PerCP	NB300-515PCP
mFluor Violet 450 SE	NB300-515MFV450
mFluor Violet 500 SE	NB300-515MFV500
mFluor Violet 610 SE	NB300-515MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB300-515-0.025ml

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NB300-530

AHR Antibody (RPT1)

(2 Publications)

100uL

NB300-530

Immunohistochemistry-Paraffin: AHR Antibody (RPT1) [NB300-530] - Cancer biopsies of deparaffinized Human breast carcinoma tissue at a dilution of 1:100.

Western Blot: AHR Antibody (RPT1) [NB300-530] - Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an Aryl Hydrocarbon Receptor monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:20,000 for 1 hour.

Immunocytochemistry/Immunofluorescence: AHR Antibody (RPT1) [NB300-530] - Analysis of Aryl Hydrocarbon Receptor in NIH-3T3 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Aryl Hydrocarbon Receptor monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Aryl Hydrocarbon Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.

Immunocytochemistry/Immunofluorescence: AHR Antibody (RPT1) [NB300-530] - Analysis of Aryl Hydrocarbon Receptor in MCF-7 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Aryl Hydrocarbon Receptor monoclonal antibody at a dilution of 1:100 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Aryl Hydrocarbon Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.

Immunohistochemistry-Paraffin: AHR Antibody (RPT1) [NB300-530] - Normal biopsies of deparaffinized Human skeletal muscle tissue at a dilution of 1:20.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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AF6697

AHR Antibody [Unconjugated]

(6 Publications)

100 μg

AF6697

HAF016). A specific band was detected for AHR at approximately 110 kDa (as indicated). GAPDH(Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of C2C12 mouse myoblast cell line, mouse lung tissue, Hepa 1-6 mouse hepatoma cell line, Neuro-2A mouse neuroblastoma cell line (negative control), NIH-3T3 mouse embryonic fibroblast cell line (negative control), and mouse cerebellum tissue (negative control). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse AHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6697) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF016). A specific band was detected for AHR at approximately 110 kDa (as indicated). GAPDH(Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of C2C12 mouse myoblast cell line, mouse lung tissue, Hepa 1-6 mouse hepatoma cell line, Neuro-2A mouse neuroblastoma cell line (negative control), NIH-3T3 mouse embryonic fibroblast cell line (negative control), and mouse cerebellum tissue (negative control). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse AHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6697) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for AHR at approximately 110 kDa (as indicated). GAPDH(Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

AHR was detected in immersion fixed C2C12 mouse myoblast cell line using Sheep Anti-Mouse AHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6697) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # <a class=

NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

<b>Biological Strategies Validation. </b>HIF1 alpha and AhR mediate butyrate induction of IL-22 in CD4+ T cells.a WT CD4+ T cells were activated with anti-CD3/CD28 mAbs under Th1 conditions ± butyrate (0.5 mM) for 2 days (n = 3 biologically independent samples per group). RNA sequencing was performed. Hif1 alpha , Ahr, and Prdm1 were shown in heatmap. b–f CD4+ T cells were activated with anti-CD3/CD28 mAbs ± butyrate (0.5 mM) under Th1 conditions (n = 3/group). Hif1a (b) and Ahr (c) were analyzed by qRT-PCR. HIF1 alpha (d) and AhR (e) protein was analyzed by western blot on day 2. HIF1 alpha activity was measured using HIF1 alpha Transcription Factor Assay Kit (f). g Raw 264.7 cells were transduced with XRE/AhR Luciferase Reporter Gene Lentivirus, and treated ± butyrate (0.5 mM) 3 days post transduction. AhR activity was assessed by luciferase. h–j Cbir1 Tg CD4+ T cells were activated with APCs and Cbir1 peptide under Th1 conditions with butyrate (0.5 mM) ± YC-1 (5 µM) or/and CH-223191 (3 µM) for 60 h (n = 3/group). IL-22 mRNA (h) and protein (i) were measured by qRT-PCR and ELISA. j IL-22 was measured by flow cytometry on day 5. k WT and HIF1 alpha −/− CD4+ T cells were activated with anti-CD3/CD28 mAbs ± butyrate (0.5 mM) for 5 days (n = 3/group). IL-22 was assessed by flow cytometry. l, m CD4+ T cells were activated with anti-CD3/CD28 mAbs under Th1 conditions with or without butyrate (0.5 mM), AR420626 (5 µM), or TSA (10 nM) for 60 h (n = 3/group). Hif1a (l) and Ahr (m) were measured by qRT-PCR. One representative of three independent experiments was shown (b–m). Data were expressed as mean ± SD. Statistical significance was tested by two-tailed unpaired Student t-test (b–g) or two-tailed one-way ANOVA (h–m). b **p = 0.0033 (24 h), ***p = 0.0002 (36 h), **p = 0.0032 (48 h), *p = 0.0310 (60 h); c *p = 0.0338 (24 h), **p = 0.0054 (36 h), ***p = 0.0003 (48 h), ***p = 0.0007 (60 h); d *p = 0.0178; e *p = 0.0325; f *p = 0.0273; g *p = 0.0435; h ****p < 0.0001; i ****p < 0.0001, ***p = 0.0002 (butyrate + YC-1 vs butyrate), ***p = 0.0006; j

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF6697U-100UG
Alexa Fluor 405	AF6697V-100UG
Alexa Fluor 488	IC6697G-100UG
Alexa Fluor 532	AF6697X-100UG
Alexa Fluor 594	AF6697T-100UG
Alexa Fluor 647	AF6697R-100UG
Alexa Fluor 700	AF6697N-100UG
Alexa Fluor 750	AF6697S-100UG
Alexa Fluor Plus 405	AF6697AFP405-100UG
Alexa Fluor Plus 488	AF6697AFP488-100UG
Alexa Fluor Plus 555	AF6697AFP555-100UG
Alexa Fluor Plus 594	AF6697AFP594-100UG
Alexa Fluor Plus 647	AF6697AFP647-100UG
Alexa Fluor Plus 680	AF6697AFP680-100UG
Biotin	AF6697B
CoraFluor 1	AF6697CL1
DyLight 350	AF6697D
DyLight 405	AF6697E
DyLight 488	AF6697K
DyLight 550	AF6697L
DyLight 594	AF6697M
DyLight 650	AF6697W
DyLight 680	AF6697Y
DyLight 755	AF6697Z
FITC	AF6697F
HRP	AF6697H
Janelia Fluor 525	AF6697JF525
Janelia Fluor 549	AF6697I
Janelia Fluor 646	AF6697J
Janelia Fluor 669	AF6697JF669
PerCP	AF6697C
mFluor Violet 450 SE	AF6697MFV450
mFluor Violet 500 SE	AF6697MFV500
mFluor Violet 610 SE	AF6697MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC6697G-100UG
Carrier Free	AF6697-SP

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H00000196-M02

AHR Antibody (3B12) - Azide and BSA Free

(5 Publications)

0.1 mg

H00000196-M02

Western Blot: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR expression in COLO 320 HSR ( Cat # L020V1 ).

Western Blot: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR over-expressed 293 cell line, cotransfected with AHR Validated Chimera RNAi ( Cat # H00000196-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with AHR monoclonal antibody (M02), clone 3B12 (Cat # H00000196-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Immunoprecipitation: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR transfected lysate using anti-AHR monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with AHR MaxPab rabbit polyclonal antibody.

ELISA: AHR Antibody (3B12) [H00000196-M02] - Detection limit for recombinant GST tagged AHR is approximately 0.03ng/ml as a capture antibody.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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AF6185

AHR Antibody [Unconjugated]

(1 Publication)

100 μg

AF6185

HAF016). A specific band was detected for AHR at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and PC-3 human prostate cancer cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human AHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6185) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF016). A specific band was detected for AHR at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and PC-3 human prostate cancer cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human AHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6185) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for AHR at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

AHR was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human AHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6185) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # <a class=

NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_lightbox">

HeLa human cervical epithelial carcinoma cell line was stained with Sheep Anti-Human AHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6185, filled histogram) or isotype control antibody (Catalog # <a class=

5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_thumb" />

HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of PC-3 human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for AHR at approximately 109 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human AHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6185) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

HAF016). A specific band was detected for AHR at approximately 100 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # 2275-PC-100) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Genetic Strategies Validation. </b>Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and AHR knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human AHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6185) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF016). A specific band was detected for AHR at approximately 100 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # 2275-PC-100) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF6185U-100UG
Alexa Fluor 405	AF6185V-100UG
Alexa Fluor 488	AF6185G-100UG
Alexa Fluor 532	AF6185X-100UG
Alexa Fluor 594	AF6185T-100UG
Alexa Fluor 647	AF6185R-100UG
Alexa Fluor 700	AF6185N-100UG
Alexa Fluor 750	AF6185S-100UG
Alexa Fluor Plus 405	AF6185AFP405-100UG
Alexa Fluor Plus 488	AF6185AFP488-100UG
Alexa Fluor Plus 555	AF6185AFP555-100UG
Alexa Fluor Plus 594	AF6185AFP594-100UG
Alexa Fluor Plus 647	AF6185AFP647-100UG
Alexa Fluor Plus 680	AF6185AFP680-100UG
Allophycocyanin	AF6185A
Biotin	AF6185B
CoraFluor 1	AF6185CL1
DyLight 350	AF6185D
DyLight 405	AF6185E
DyLight 488	AF6185K
DyLight 550	AF6185L
DyLight 594	AF6185M
DyLight 650	AF6185W
DyLight 680	AF6185Y
DyLight 755	AF6185Z
FITC	AF6185F
HRP	AF6185H
Janelia Fluor 525	AF6185JF525
Janelia Fluor 549	AF6185I
Janelia Fluor 646	AF6185J
Janelia Fluor 669	AF6185JF669
PE	AF6185P
PerCP	AF6185C
mFluor Violet 450 SE	AF6185MFV450
mFluor Violet 500 SE	AF6185MFV500
mFluor Violet 610 SE	AF6185MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF6185-SP

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MAB6185

AHR Antibody (684217) [Unconjugated]

(2 Publications)

100 μg

MAB6185

CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

AHR was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Mouse Anti-Human AHR Monoclonal Antibody (Catalog # MAB6185) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="AHR was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Mouse Anti-Human AHR Monoclonal Antibody (Catalog # MAB6185) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB6185U-100UG
Alexa Fluor 405	FAB6185V-100UG
Alexa Fluor 488	FAB6185G-100UG
Alexa Fluor 532	FAB6185X-100UG
Alexa Fluor 594	FAB6185T-100UG
Alexa Fluor 647	FAB6185R-100UG
Alexa Fluor 700	FAB6185N-100UG
Alexa Fluor 750	FAB6185S-100UG
Alexa Fluor Plus 405	FAB6185AFP405-100UG
Alexa Fluor Plus 488	FAB6185AFP488-100UG
Alexa Fluor Plus 555	FAB6185AFP555-100UG
Alexa Fluor Plus 594	FAB6185AFP594-100UG
Alexa Fluor Plus 647	FAB6185AFP647-100UG
Alexa Fluor Plus 680	FAB6185AFP680-100UG
Allophycocyanin	FAB6185A
Biotin	FAB6185B
CoraFluor 1	FAB6185CL1
DyLight 350	FAB6185D
DyLight 405	FAB6185E
DyLight 488	FAB6185K
DyLight 550	FAB6185L
DyLight 594	FAB6185M
DyLight 650	FAB6185W
DyLight 680	FAB6185Y
DyLight 755	FAB6185Z
FITC	FAB6185F
HRP	FAB6185H
Janelia Fluor 525	FAB6185JF525
Janelia Fluor 549	FAB6185I
Janelia Fluor 646	FAB6185J
Janelia Fluor 669	FAB6185JF669
PE	FAB6185P
PerCP	FAB6185C
mFluor Violet 450 SE	FAB6185MFV450
mFluor Violet 500 SE	FAB6185MFV500
mFluor Violet 610 SE	FAB6185MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB6185-SP

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NBP1-89975

AHR Antibody - BSA Free

0.1 ml

NBP1-89975

<b>Orthogonal Strategies Validation. </b>Staining of human urinary bladder shows strong nuclear positivity in urothelial cells.

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Staining of human skin shows moderate nuclear positivity in squamous epithelial cells.

Staining of human lung shows moderate nuclear positivity in subset of macrophages.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-89975-25ul

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NBP1-89974

AHR Antibody - BSA Free

0.1 ml

NBP1-89974

Staining of human cerebral cortex shows moderate cytoplasmic and nuclear positivity in neuronal cells.

Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-89974-25ul

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NBP3-19574

AHR Antibody (S07-1H9)

100 ul

NBP3-19574

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP2-66669

Human AHR ELISA Kit (Chemiluminescence)

1 Kit

NBP2-66669

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Standard Curve:

78.13-5000 pg/mL

Sensitivity:

46.88 pg/mL

Sample Type:

Serum, Plasma, Cell Supernatant, Tissue Homogenate

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

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