291-G1), Recombinant Mouse IGF-I/IGF-1 (Catalog #
791-MG), Recombinant Rat IGF-I/IGF-1 (Catalog #
4326-RG), and Recombinant Human IGF-II (Catalog #
292-G2). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB291) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #
HAF018). A specific band was detected for IGF-I/IGF-1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 3." class="big_lightbox">

291-G1), Recombinant Mouse IGF-I/IGF-1 (Catalog #
791-MG), Recombinant Rat IGF-I/IGF-1 (Catalog #
4326-RG), and Recombinant Human IGF-II (Catalog #
292-G2). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB291) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #
HAF018). A specific band was detected for IGF-I/IGF-1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 3." alt="Western blot shows 25 ng of Recombinant Human IGF-I/IGF-1 (Catalog #
291-G1), Recombinant Mouse IGF-I/IGF-1 (Catalog #
791-MG), Recombinant Rat IGF-I/IGF-1 (Catalog #
4326-RG), and Recombinant Human IGF-II (Catalog #
292-G2). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB291) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #
HAF018). A specific band was detected for IGF-I/IGF-1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 3." class="big_thumb" />
291-G1) was serially diluted and captured by Mouse Anti-Human IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB291) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog #
AF-291-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_lightbox">

291-G1) was serially diluted and captured by Mouse Anti-Human IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB291) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog #
AF-291-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_thumb" />